Antibody-free isolation of rare cancer cells from blood based on 3D lateral dielectrophoresis

Lab on a Chip ◽  
2015 ◽  
Vol 15 (14) ◽  
pp. 2950-2959 ◽  
Author(s):  
I-Fang Cheng ◽  
Wei-Lun Huang ◽  
Tzu-Ying Chen ◽  
Chien-Wei Liu ◽  
Yu-De Lin ◽  
...  
Keyword(s):  
Cancer Cells ◽  
High Throughput ◽  
Microfluidic Chip ◽  
Rare Cancer

We present an antibody-free approach for high throughput and purity dielectrophoretic isolation of CTCs from blood in a microfluidic chip.

High-purity Isolation for Genotyping Rare Cancer Cells from Blood Using a Microfluidic Chip Cell Sorter

2021 ◽  
Vol 42 (1) ◽  
pp. 407-417
Author(s):  
MIO IKEDA ◽  
YASUHIRO KOH ◽  
JUN OYANAGI ◽  
SHUNSUKE TERAOKA ◽  
MASAYUKI ISHIGE ◽  
...  
Keyword(s):  
Cancer Cells ◽  
Microfluidic Chip ◽  
High Purity ◽  
Rare Cancer ◽  
Cell Sorter

Rhipsalis (Cactaceae)-like Hierarchical Structure Based Microfluidic Chip for Highly Efficient Isolation of Rare Cancer Cells

2016 ◽  
Vol 8 (49) ◽  
pp. 33457-33463 ◽  
Author(s):  
Shuangqian Yan ◽  
Xian Zhang ◽  
Xiaofang Dai ◽  
Xiaojun Feng ◽  
Wei Du ◽  
...  
Keyword(s):  
Cancer Cells ◽  
Hierarchical Structure ◽  
Microfluidic Chip ◽  
Rare Cancer ◽  
Highly Efficient

Effective reduction of non-specific binding of blood cells in a microfluidic chip for isolation of rare cancer cells

2018 ◽  
Vol 6 (11) ◽  
pp. 2871-2880 ◽  
Author(s):  
Dan Yu ◽  
Ling Tang ◽  
Ziye Dong ◽  
Kevin A. Loftis ◽  
Zhenya Ding ◽  
...  
Keyword(s):  
Cancer Cells ◽  
Microfluidic Chip ◽  
Blood Cells ◽  
Specific Binding ◽  
Rare Cancer ◽  
Effective Reduction

Effective reducing non-specific binding of blood cells in microchips by sheathing the surface with a biodegradable multilayer nanofilm.

Visual and high-throughput detection of cancer cells using a graphene oxide-based FRET aptasensing microfluidic chip

Lab on a Chip ◽  
2012 ◽  
Vol 12 (22) ◽  
pp. 4864 ◽  
Author(s):  
Lili Cao ◽  
Liwei Cheng ◽  
Zhengyong Zhang ◽  
Yi Wang ◽  
Xianxia Zhang ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Cancer Cells ◽  
High Throughput ◽  
Microfluidic Chip ◽  
High Throughput Detection

scholarly journals High-throughput single-cell chromatin accessibility CRISPR screens enable unbiased identification of regulatory networks in cancer

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Sarah E. Pierce ◽  
Jeffrey M. Granja ◽  
William J. Greenleaf
Keyword(s):  
Transcription Factor ◽  
Single Cell ◽  
Cancer Cells ◽  
High Throughput ◽  
Regulatory Networks ◽  
Temporal Dynamics ◽  
Chromatin Accessibility ◽  
Regulatory Regions ◽  
Factor Binding ◽  
Genome Wide

AbstractChromatin accessibility profiling can identify putative regulatory regions genome wide; however, pooled single-cell methods for assessing the effects of regulatory perturbations on accessibility are limited. Here, we report a modified droplet-based single-cell ATAC-seq protocol for perturbing and evaluating dynamic single-cell epigenetic states. This method (Spear-ATAC) enables simultaneous read-out of chromatin accessibility profiles and integrated sgRNA spacer sequences from thousands of individual cells at once. Spear-ATAC profiling of 104,592 cells representing 414 sgRNA knock-down populations reveals the temporal dynamics of epigenetic responses to regulatory perturbations in cancer cells and the associations between transcription factor binding profiles.

Live cell, image-based high-throughput screen to quantitate p53 stabilization and viability in human papillomavirus positive cancer cells

Virology ◽  
2021 ◽  
Author(s):  
Gustavo Martínez-Noël ◽  
Valdimara Corrêa Vieira ◽  
Patricia Szajner ◽  
Erin M. Lilienthal ◽  
Rebecca E. Kramer ◽  
...  
Keyword(s):  
Human Papillomavirus ◽  
Cancer Cells ◽  
High Throughput ◽  
Live Cell ◽  
High Throughput Screen ◽  
Cell Image ◽  
Live Cell Image

scholarly journals Temperature-controlled MPa-pressure ultrasonic cell manipulation in a microfluidic chip

Lab on a Chip ◽  
2015 ◽  
Vol 15 (16) ◽  
pp. 3341-3349 ◽  
Author(s):  
Mathias Ohlin ◽  
Ida Iranmanesh ◽  
Athanasia E. Christakou ◽  
Martin Wiklund
Keyword(s):  
Lung Cancer ◽  
High Pressure ◽  
Cancer Cells ◽  
Microfluidic Chip ◽  
Acoustic Streaming ◽  
Cell Manipulation ◽  
Wave Trapping ◽  
Ultrasonic Standing Wave ◽  
Temperature Controlled

We study the effect of 1 MPa-pressure ultrasonic-standing-wave trapping of cells during one hour in a fully temperature- and acoustic streaming-controlled microfluidic chip, and conclude that the viability of lung cancer cells are not affected by this high-pressure, long-term acoustophoresis treatment.

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