High-purity Isolation for Genotyping Rare Cancer Cells from Blood Using a Microfluidic Chip Cell Sorter

2021 ◽  
Vol 42 (1) ◽  
pp. 407-417
Author(s):  
MIO IKEDA ◽  
YASUHIRO KOH ◽  
JUN OYANAGI ◽  
SHUNSUKE TERAOKA ◽  
MASAYUKI ISHIGE ◽  
...  
Keyword(s):  
Cancer Cells ◽  
Microfluidic Chip ◽  
High Purity ◽  
Rare Cancer ◽  
Cell Sorter

Antibody-free isolation of rare cancer cells from blood based on 3D lateral dielectrophoresis

Lab on a Chip ◽  
2015 ◽  
Vol 15 (14) ◽  
pp. 2950-2959 ◽  
Author(s):  
I-Fang Cheng ◽  
Wei-Lun Huang ◽  
Tzu-Ying Chen ◽  
Chien-Wei Liu ◽  
Yu-De Lin ◽  
...  
Keyword(s):  
Cancer Cells ◽  
High Throughput ◽  
Microfluidic Chip ◽  
Rare Cancer

We present an antibody-free approach for high throughput and purity dielectrophoretic isolation of CTCs from blood in a microfluidic chip.

Rhipsalis (Cactaceae)-like Hierarchical Structure Based Microfluidic Chip for Highly Efficient Isolation of Rare Cancer Cells

2016 ◽  
Vol 8 (49) ◽  
pp. 33457-33463 ◽  
Author(s):  
Shuangqian Yan ◽  
Xian Zhang ◽  
Xiaofang Dai ◽  
Xiaojun Feng ◽  
Wei Du ◽  
...  
Keyword(s):  
Cancer Cells ◽  
Hierarchical Structure ◽  
Microfluidic Chip ◽  
Rare Cancer ◽  
Highly Efficient

Effective reduction of non-specific binding of blood cells in a microfluidic chip for isolation of rare cancer cells

2018 ◽  
Vol 6 (11) ◽  
pp. 2871-2880 ◽  
Author(s):  
Dan Yu ◽  
Ling Tang ◽  
Ziye Dong ◽  
Kevin A. Loftis ◽  
Zhenya Ding ◽  
...  
Keyword(s):  
Cancer Cells ◽  
Microfluidic Chip ◽  
Blood Cells ◽  
Specific Binding ◽  
Rare Cancer ◽  
Effective Reduction

Effective reducing non-specific binding of blood cells in microchips by sheathing the surface with a biodegradable multilayer nanofilm.

Abstract 2223: High-purity isolation method of rare cells for molecular analyses using microfluidic chip type cell sorter

2019 ◽  
Author(s):  
Yasuhiro Koh ◽  
Mio Ikeda ◽  
Shunsuke Teraoka ◽  
Masayuki Ishige ◽  
Yuu Fujimura ◽  
...  
Keyword(s):  
Microfluidic Chip ◽  
High Purity ◽  
Isolation Method ◽  
Cell Sorter ◽  
Molecular Analyses ◽  
Chip Type ◽  
Rare Cells

Abstract 2223: High-purity isolation method of rare cells for molecular analyses using microfluidic chip type cell sorter

2019 ◽  
Author(s):  
Yasuhiro Koh ◽  
Mio Ikeda ◽  
Shunsuke Teraoka ◽  
Masayuki Ishige ◽  
Yuu Fujimura ◽  
...  
Keyword(s):  
Microfluidic Chip ◽  
High Purity ◽  
Isolation Method ◽  
Cell Sorter ◽  
Molecular Analyses ◽  
Chip Type ◽  
Rare Cells

scholarly journals Temperature-controlled MPa-pressure ultrasonic cell manipulation in a microfluidic chip

Lab on a Chip ◽  
2015 ◽  
Vol 15 (16) ◽  
pp. 3341-3349 ◽  
Author(s):  
Mathias Ohlin ◽  
Ida Iranmanesh ◽  
Athanasia E. Christakou ◽  
Martin Wiklund
Keyword(s):  
Lung Cancer ◽  
High Pressure ◽  
Cancer Cells ◽  
Microfluidic Chip ◽  
Acoustic Streaming ◽  
Cell Manipulation ◽  
Wave Trapping ◽  
Ultrasonic Standing Wave ◽  
Temperature Controlled

We study the effect of 1 MPa-pressure ultrasonic-standing-wave trapping of cells during one hour in a fully temperature- and acoustic streaming-controlled microfluidic chip, and conclude that the viability of lung cancer cells are not affected by this high-pressure, long-term acoustophoresis treatment.

Abstract 188: Deep learning enables label-free profiling of the tumor microenvironment and enrichment of rare cancer cells

2021 ◽  
Author(s):  
Mahyar Salek ◽  
Hou-pu Chou ◽  
Prashast Khandelwal ◽  
Krishna P. Pant ◽  
Thomas J. Musci ◽  
...  
Keyword(s):  
Deep Learning ◽  
Tumor Microenvironment ◽  
Cancer Cells ◽  
Rare Cancer ◽  
Label Free

A Microfluidic Platform for On-Chip Analysis of Circulating Tumor Cells

2021 ◽  
Author(s):  
Jeff Darabi ◽  
Joseph Schober
Keyword(s):  
Cancer Cells ◽  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Whole Blood ◽  
Peripheral Blood ◽  
Automated Image Analysis ◽  
Rare Cancer ◽  
On Chip ◽  
Chip Analysis ◽  
Selection Of

Abstract Studies have shown that primary tumor sites begin shedding cancerous cells into peripheral blood at early stages of cancer, and the presence and frequency of circulating tumor cells (CTCs) in blood is directly proportional to disease progression. The challenge is that the concentration of the CTCs in peripheral blood may be extremely low. In the past few years, several microfluidic-based concepts have been investigated to isolate CTCs from whole blood. However, these devices are generally hampered by complex fabrication processes and very low volumetric throughputs, which may not be practical for rapid clinical applications. This paper presents a high-performance yet simple magnetophoretic microfluidic chip for the enrichment and on-chip analysis of rare CTCs from blood. Microscopic and flow cytometric assays developed for selection of cancer cell lines, selection of monoclonal antibodies, and optimization of bead coupling are discussed. Additionally, on-chip characterization of rare cancer cells using high resolution immunofluorescence microscopy and modeling results for prediction of CTC capture length are presented. The device has the ability to interface directly with on-chip pre and post processing modules such as mixing, incubation, and automated image analysis systems. These features will enable us to isolate rare cancer cells from whole blood and detect them on the chip with subcellular resolution.

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