Visual and high-throughput detection of cancer cells using a graphene oxide-based FRET aptasensing microfluidic chip

Lab on a Chip ◽  
2012 ◽  
Vol 12 (22) ◽  
pp. 4864 ◽  
Author(s):  
Lili Cao ◽  
Liwei Cheng ◽  
Zhengyong Zhang ◽  
Yi Wang ◽  
Xianxia Zhang ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Cancer Cells ◽  
High Throughput ◽  
Microfluidic Chip ◽  
High Throughput Detection

Antibody-free isolation of rare cancer cells from blood based on 3D lateral dielectrophoresis

Lab on a Chip ◽  
2015 ◽  
Vol 15 (14) ◽  
pp. 2950-2959 ◽  
Author(s):  
I-Fang Cheng ◽  
Wei-Lun Huang ◽  
Tzu-Ying Chen ◽  
Chien-Wei Liu ◽  
Yu-De Lin ◽  
...  
Keyword(s):  
Cancer Cells ◽  
High Throughput ◽  
Microfluidic Chip ◽  
Rare Cancer

We present an antibody-free approach for high throughput and purity dielectrophoretic isolation of CTCs from blood in a microfluidic chip.

scholarly journals Multiplex and On-site PCR detection of swine diseases based on the microfluidic chip system

2020 ◽  
Author(s):  
Yan Jiang ◽  
Shan Jiang ◽  
Yue Wu ◽  
Bin Zhou ◽  
Kaiming Wang ◽  
...  
Keyword(s):  
High Throughput ◽  
Microfluidic Chip ◽  
Detection System ◽  
Pcr Detection ◽  
Clinical Samples ◽  
Disease Detection ◽  
Pathogenic Microorganisms ◽  
Inspection And Quarantine ◽  
High Throughput Detection ◽  
Swine Diseases

Abstract Background: At present, the process of inspection and quarantine starts with sampling at the customs port, continues with transporting the samples to the central laboratory for inspection experiments, and ends with the inspected results being fed back to the port. This process takes a rather long time, has the risks of degradation of biological samples and generation of pathogenic microorganisms, and does not meet the rapid on-site detection demand. Therefore, development of a technology for rapid and high-throughput detection of pathogenic microorganisms at the customs port is of great significance. This study was to develop a microfluidic chip to be applied to rapid high-throughput detection for swine disease with higher accuracy and lower risk of spreading pathogenic microorganisms during transportation. Results: PCR technology has the advantages of high accurate and sensitivity in disease detection, clinical testing and food quarantine, so it plays an important role in customs inspection. However, the traditional PCR detection instrument has a large size, is time-consuming and has strict requirements on the experimental environment, which greatly limit its application in on-site testing. In this paper, the positive nucleic acid of four swine diseases were detected by a portable and rapid microfluidic PCR system, which could achieve a on-site real-time quantitative PCR detection. Eight clinical samples were detected together on the microfluidic chip in the system, and the detection results were obtained in about an hour. The detection limit of this microfluidic PCR detection system was as low as 1 copies/μL. The results show that the high sensitivity and specificity of the microfluidic PCR detection system in disease detection will play an important role in customs inspection and quarantine during customs clearance. Conclusion: The microfluidic PCR detection system established in this study could meet the requirements for rapid detection of samples at the customs port The new method can avoid the risky process of transporting the samples from the sampling site to the testing lab, and drastically reducing the inspection cycle, and would enable parallel inspections on one chip which greatly raising the efficiency of inspection.

scholarly journals Rapid, amplification-free and high-throughput SARS-CoV-2 RNA detection via reduced-graphene-oxide based fluorescence assay

2022 ◽  
Author(s):  
Yu Zhang ◽  
Min Wang ◽  
Yujin Chu ◽  
Yingkuan Han ◽  
Le Qiang ◽  
...  
Keyword(s):  
Public Health ◽  
Graphene Oxide ◽  
Infectious Diseases ◽  
High Throughput ◽  
Low Cost ◽  
Global Public Health ◽  
Rna Detection ◽  
Reduced Graphene ◽  
High Throughput Detection ◽  
Rapid Amplification

The infectious diseases caused by the SARS-CoV-2 virus have been global public health threats and caught worldwide concern. Until now, rapid, low-cost and high-throughput detection of the COVID-19 virus is...

High-throughput Detection of Protein Kinase Activities in Cell Lysate Based on the Aggregation of Gold Nanoparticles with Peptides

2009 ◽  
Author(s):  
Yoshiki Katayama ◽  
Hirotaro Kitazaki ◽  
Jeong-Hun Kang ◽  
Xiaoming Han ◽  
Takeshi Mori ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Protein Kinase ◽  
High Throughput ◽  
Cell Lysate ◽  
High Throughput Detection

scholarly journals High-throughput single-cell chromatin accessibility CRISPR screens enable unbiased identification of regulatory networks in cancer

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Sarah E. Pierce ◽  
Jeffrey M. Granja ◽  
William J. Greenleaf
Keyword(s):  
Transcription Factor ◽  
Single Cell ◽  
Cancer Cells ◽  
High Throughput ◽  
Regulatory Networks ◽  
Temporal Dynamics ◽  
Chromatin Accessibility ◽  
Regulatory Regions ◽  
Factor Binding ◽  
Genome Wide

AbstractChromatin accessibility profiling can identify putative regulatory regions genome wide; however, pooled single-cell methods for assessing the effects of regulatory perturbations on accessibility are limited. Here, we report a modified droplet-based single-cell ATAC-seq protocol for perturbing and evaluating dynamic single-cell epigenetic states. This method (Spear-ATAC) enables simultaneous read-out of chromatin accessibility profiles and integrated sgRNA spacer sequences from thousands of individual cells at once. Spear-ATAC profiling of 104,592 cells representing 414 sgRNA knock-down populations reveals the temporal dynamics of epigenetic responses to regulatory perturbations in cancer cells and the associations between transcription factor binding profiles.

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