Barley malt increases hindgut and portal butyric acid, modulates gene expression of gut tight junction proteins and Toll-like receptors in rats fed high-fat diets, but high advanced glycation end-products partially attenuate the effects

2015 ◽  
Vol 6 (9) ◽  
pp. 3165-3176 ◽  
Author(s):  
Yadong Zhong ◽  
Cristina Teixeira ◽  
Nittaya Marungruang ◽  
Watina Sae-Lim ◽  
Eden Tareke ◽  
...  
Keyword(s):  
Butyric Acid ◽  
Tight Junction Proteins ◽  
High Fat ◽  
Advanced Glycation ◽  
Barley Malt ◽  
Glycation End Products ◽  
High Fat Diets ◽  
End Products ◽  
Fat Diets ◽  
Junction Proteins

Barley malt has been shown to give high levels of butyric acid in the cecum and serum of rats.

scholarly journals Monobutyrin and Monovalerin Affect Brain Short-Chain Fatty Acid Profiles and Tight-Junction Protein Expression in ApoE-Knockout Rats Fed High-Fat Diets

Nutrients ◽  
2020 ◽  
Vol 12 (4) ◽  
pp. 1202 ◽  
Author(s):  
Thao Duy Nguyen ◽  
Frida Fåk Hållenius ◽  
Xue Lin ◽  
Margareta Nyman ◽  
Olena Prykhodko
Keyword(s):  
Tight Junction ◽  
Hdl Cholesterol ◽  
Short Chain ◽  
Liver Cholesterol ◽  
Tight Junction Proteins ◽  
High Fat ◽  
High Fat Diets ◽  
Fat Diets ◽  
The Brain ◽  
Junction Proteins

Monobutyrin (MB) and monovalerin (MV), esters of short-chain fatty acids (SCFAs), have previously been shown to reduce liver cholesterol and inflammation in conventional rats fed high-fat diets. This study explored the potential effects of MB and MV in hypercholesterolemic apolipoprotein E-knockout (ApoE-/-) rats. ApoE-/- rats were fed three high-fat (HF) diets, pure or supplemented with MB or MV (1%), for 5 weeks. One group of conventional rats (C) was also fed the pure high-fat diet and another group of ApoE-/- rats a low-fat (LF) diet. Blood and liver lipids, urinary lactulose/mannitol, SCFAs (blood and brain), tight junction proteins (small intestine and brain), and inflammation-related markers (blood, brain, and liver) were analyzed. MV supplementation elevated serum high-density lipoprotein (HDL) cholesterol and valeric acid concentration (p < 0.05), while the amounts of isovaleric acid in the brain were reduced (p < 0.05). MB increased butyric acid amounts in the brain, while the plasma concentration of interleukin 10 (IL-10) was lowered (p < 0.05). Both MV and MB upregulated the expression of occludin and zonula occludens-1 (ZO-1) in the brain (p < 0.05). Supplementation of MB or MV affected HDL cholesterol, the expression of tight junction proteins, and SCFA profiles. MB and MV may therefore be promising supplements to attenuate lipid metabolic disorders caused by high-fat intake and genetic deficiency.

scholarly journals Black soybean tempeh and purple sweet potato improve sperm quality in streptozotocin-induced diabetic rats

2020 ◽  
Vol 13 (11) ◽  
pp. 2534-2540
Author(s):  
Abdul Gofur ◽  
Agung Witjoro ◽  
Siti Nur Arifah ◽  
Mochammad Fitri Atho'illah ◽  
Yuslinda Annisa ◽  
...  
Keyword(s):  
Sweet Potato ◽  
Sperm Quality ◽  
Diabetic Rats ◽  
High Fat ◽  
Advanced Glycation ◽  
Black Soybean ◽  
High Fat Diets ◽  
Purple Sweet Potato ◽  
Fat Diets ◽  
Rage Expression

Background and Aim: Hyperglycemia increases advanced glycation end-product (AGE) production, and the activity of receptor for AGE (RAGE) in testis, which leads to testicular histopathological damage and infertility. This research investigated the effect of black soybean tempeh (BST), purple sweet potato (PSP), and its combination on AGE and RAGE expression and spermatozoa quality in streptozotocin (STZ)-induced diabetic rats. Materials and Methods: The rats were given high-fat diets for 5 weeks, then were injected intraperitoneally with multiple low doses of STZ (30 mg/kg body weight). Diabetes mellitus (DM) rats were divided into seven groups: DM, DM+glibenclamide, DM+BST, DM+PSP, and DM+combination of BST and PSP in ratio 1:3, 2:2, and 3:1 as C1, C2, and C3, respectively. The rats were treated for 30 days. Testicular AGE and RAGE expression and spermatozoa quality were measured. Results: The combination of BST and PSP significantly decreased AGE and RAGE expression in testicular organs and improved spermatozoa quality compared to the normal group. Conclusion: The combination of BST and PSP can be used as future alternatives to improve spermatozoa quality in DM patients.

Mangiferin suppressed advanced glycation end products (AGEs) through NF-κB deactivation and displayed anti-inflammatory effects in streptozotocin and high fat diet-diabetic cardiomyopathy rats

2016 ◽  
Vol 94 (3) ◽  
pp. 332-340 ◽  
Author(s):  
Jun Hou ◽  
Dezhi Zheng ◽  
Gabriel Fung ◽  
Haoyu Deng ◽  
Lin Chen ◽  
...  
Keyword(s):  
Inflammatory Cytokines ◽  
Diabetic Cardiomyopathy ◽  
Advanced Glycation End Products ◽  
High Fat Diet ◽  
Nuclear Translocation ◽  
High Fat ◽  
Advanced Glycation ◽  
Glycation End Products ◽  
End Products ◽  
Mrna And Protein Expression

Given the importance of the aggregation of advanced glycation end products (AGEs) and cardiac inflammation in the onset and progression of diabetic cardiomyopathy (DCM), our objective in this study was to demonstrate the cardioprotective effect of mangiferin, an antidiabetic and anti-inflammatory agent, on diabetic rat model. The DCM model was established by a high-fat diet and a low dose of streptozotocin. DCM rats were treated orally with mangiferin (20 mg/kg) for 16 weeks. Serum and left ventricular myocardium were collected for determination of inflammatory cytokines. AGEs mRNA and protein expression of nuclear factor kappa B (NF-κB) and receptor for AGEs (RAGE) in myocardium were assayed by real-time PCR and Western blot. ROS levels were measured by dihydroethidium fluorescence staining. NF-κB binding activity was assayed by TransAM NF-κB p65 ELISA kit. Chronic treatment with mangiferin decreased the levels of myocardial enzymes (CK-MB, LDH) and inflammatory mediators (TNF-α, IL-1β). Meanwhile, NF-κB is inhibited by the reduction of nuclear translocation of p65 subunit, and mangiferin reduced AGE production and decreased the mRNA and protein expression of RAGE in DCM rats. Our data indicated that mangiferin could significantly ameliorate DCM by preventing the release of inflammatory cytokines, and inhibiting ROS accumulation, AGE/RAGE production, and NF-κB nuclear translocation, suggesting that mangiferin treatment might be beneficial in DCM.

scholarly journals Contribution of dietary advanced glycation end products (AGE) to circulating AGE: role of dietary fat

2015 ◽  
Vol 114 (11) ◽  
pp. 1797-1806 ◽  
Author(s):  
Kathleen E. Davis ◽  
Chandan Prasad ◽  
Parakat Vijayagopal ◽  
Shanil Juma ◽  
Beverley Adams-Huet ◽  
...  
Keyword(s):  
Advanced Glycation End Products ◽  
Test Meal ◽  
Dietary Factors ◽  
High Fat ◽  
Advanced Glycation ◽  
Low Fat ◽  
High Molecular Weight Adiponectin ◽  
Glycation End Products ◽  
End Products ◽  
High Age

AbstractThe purpose of this pilot study was to determine whether macronutrient content (low-fat v. high-fat diet) influences an indicator of advanced glycation end products (AGE), Nε carboxymethyl-lysine (CML), in the context of a 1-d, high-AGE diet. The effect of the diets on inflammatory markers was also assessed. A total of nineteen overweight and obese adults (nine men and ten women) without known disease were recruited to participate in a crossover challenge of a high-fat, high-AGE (HFHA) and low-fat, high-AGE (LFHA) diet. In each phase patients had fasting blood drawn, followed by consumption of a high-fat or low-fat breakfast test meal, then three postprandial blood draws at 1, 2 and 3 h after consuming the test meal. After consuming high-AGE meals for the remainder of the day, participants returned the next day for a follow-up analysis. A different pattern in the 3-h post-meal CML and soluble receptor for AGE response to the two diets was observed (P=0·01 and 0·05, respectively). No change in serum CML was observed following consumption of a LFHA breakfast (535 (25th–75th percentile 451–790) to 495 (25th–75th percentile 391–682) ng/ml; P=0·36), whereas a rise in CML occurred after the HFHA breakfast (463 (25th–75th percentile 428–664) to 578 (25th–75th percentile 474–865) ng/ml; P=0·05). High sensitivity C-reactive protein and high molecular weight adiponectin were not affected by either diet. These findings suggest that dietary CML may not be as important in influencing serum CML as other dietary factors. In addition, acute exposure to dietary CML may not influence inflammation in adults without diabetes or kidney disease. This is contrary to previous findings.

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