Label-free molecular beacon-based quadratic isothermal exponential amplification: a simple and sensitive one-pot method to detect DNA methyltransferase activity

2015 ◽  
Vol 51 (70) ◽  
pp. 13538-13541 ◽  
Author(s):  
Qingwang Xue ◽  
Lei Wang ◽  
Wei Jiang
Keyword(s):  
Dna Methyltransferase ◽  
Molecular Beacon ◽  
Label Free ◽  
Methyltransferase Activity ◽  
One Pot ◽  
Amplification Strategy

An illustration of the label-free molecular beacon-mediated quadratic isothermal exponential amplification strategy (LFMB-QIEA) for target Dam MTase detection.

scholarly journals An integrated-molecular-beacon based multiple exponential strand displacement amplification strategy for ultrasensitive detection of DNA methyltransferase activity

2019 ◽  
Vol 10 (8) ◽  
pp. 2290-2297 ◽  
Author(s):  
Yun-Xi Cui ◽  
Xue-Nan Feng ◽  
Ya-Xin Wang ◽  
Hui-Yu Pan ◽  
Hua Pan ◽  
...  
Keyword(s):  
Signal Amplification ◽  
Dna Methyltransferase ◽  
Molecular Beacon ◽  
Ultrasensitive Detection ◽  
Strand Displacement ◽  
Methyltransferase Activity ◽  
Strand Displacement Amplification ◽  
Multiple Signal ◽  
Amplification Strategy

An ultra-sensitive biosensor using only two DNA oligos to initiate multiple signal amplification cycles.

Detection of DNA methyltransferase activity using allosteric molecular beacons

The Analyst ◽  
2016 ◽  
Vol 141 (2) ◽  
pp. 579-584 ◽  
Author(s):  
Weiting Zhang ◽  
Xiaolong Zu ◽  
Yanling Song ◽  
Zhi Zhu ◽  
Chaoyong James Yang
Keyword(s):  
Dna Methylation ◽  
Dna Methyltransferase ◽  
Molecular Beacon ◽  
Molecular Beacons ◽  
Sensitive Detection ◽  
Methyltransferase Activity ◽  
Mtase Activity ◽  
Adenine Methylation ◽  
Methylation Patterns

Abnormal DNA methylation patterns caused by altered DNA methyltransferase (MTase) activity are closely associated with cancer. Herein, using DNA adenine methylation methyltransferase (Dam MTase) as a model analyte, we designed an allosteric molecular beacon (aMB) for sensitive detection of Dam MTase activity.

Nanopore-Based, Label-Free, and Real-Time Monitoring Assay for DNA Methyltransferase Activity and Inhibition

2017 ◽  
Vol 89 (24) ◽  
pp. 13252-13260 ◽  
Author(s):  
Sana Rauf ◽  
Ling Zhang ◽  
Asghar Ali ◽  
Jalal Ahmad ◽  
Yang Liu ◽  
...  
Keyword(s):  
Real Time ◽  
Dna Methyltransferase ◽  
Label Free ◽  
Real Time Monitoring ◽  
Methyltransferase Activity

Target-protected dumbbell molecular probe mediated cascade rolling circle amplification strategy for the sensitive assay of DNA methyltransferase activity

2016 ◽  
Vol 52 (12) ◽  
pp. 2517-2520 ◽  
Author(s):  
Haiyan Zhao ◽  
Lei Wang ◽  
Wei Jiang
Keyword(s):  
Dna Methyltransferase ◽  
Detection System ◽  
Rolling Circle Amplification ◽  
Molecular Probe ◽  
Sensitive Assay ◽  
Activity Assay ◽  
Methyltransferase Activity ◽  
Rolling Circle ◽  
Amplification Strategy ◽  
Fluorescence Detection System

A novel fluorescence detection system is developed for DNA methyltransferase activity assay based on target-protected dumbbell molecular probe mediated cascade rolling circle amplification strategy.

Sign in / Sign up

Export Citation Format

Share Document