Label-free and amplified electrogenerated chemiluminescence biosensing method for the determination of DNA methyltransferase activity using signal reagent-assembled graphene oxide

2014 ◽  
Vol 137 ◽  
pp. 454-461 ◽  
Author(s):  
Yan Li ◽  
Zhao Yan ◽  
Jianbin Zheng ◽  
Honglan Qi
Keyword(s):  
Graphene Oxide ◽  
Dna Methyltransferase ◽  
Electrogenerated Chemiluminescence ◽  
Label Free ◽  
Methyltransferase Activity

A sensitive cyclic signal amplification fluorescence strategy for determination of methyltransferase activity based on graphene oxide and RNase H

2019 ◽  
Vol 7 (29) ◽  
pp. 4520-4527 ◽  
Author(s):  
Han Zhao ◽  
Changbei Ma ◽  
Ying Yan ◽  
Mingjian Chen
Keyword(s):  
Graphene Oxide ◽  
Signal Amplification ◽  
Dna Methyltransferase ◽  
Rnase H ◽  
Activity Detection ◽  
Methyltransferase Activity ◽  
Fluorometric Method

A sensitive fluorometric method for DNA methyltransferase activity detection based on graphene oxide and RNase H-assisted signal amplification.

The determination of DNA methyltransferase activity by quenching of tris(2,2′-bipyridine)ruthenium electrogenerated chemiluminescence with ferrocene

2015 ◽  
Vol 51 (46) ◽  
pp. 9487-9490 ◽  
Author(s):  
Xiaoe Luo ◽  
Yan Li ◽  
Jianbin Zheng ◽  
Honglan Qi ◽  
Zhenxing Liang ◽  
...  
Keyword(s):  
Dna Methyltransferase ◽  
Electrogenerated Chemiluminescence ◽  
Methyltransferase Activity

An electrogenerated chemiluminescence biosensing method for the determination of DNA methyltransferase activity is developed by the quenching of tris(2,2′-bipyridine)ruthenium ECL by ferrocene.

scholarly journals A label-free biosensor based on graphene and reduced graphene oxide dual-layer for electrochemical determination of beta-amyloid biomarkers

2020 ◽  
Vol 187 (5) ◽  
Author(s):  
Jagriti Sethi ◽  
Michiel Van Bulck ◽  
Ahmed Suhail ◽  
Mina Safarzadeh ◽  
Ana Perez-Castillo ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Reduced Graphene Oxide ◽  
Limit Of Detection ◽  
Electrochemical Techniques ◽  
Differential Pulse ◽  
Electrochemical Determination ◽  
Label Free ◽  
Reduced Graphene ◽  
Electrochemically Reduced Graphene Oxide

AbstractA label-free biosensor is developed for the determination of plasma-based Aβ1–42 biomarker in Alzheimer’s disease (AD). The platform is based on highly conductive dual-layer of graphene and electrochemically reduced graphene oxide (rGO). The modification of dual-layer with 1-pyrenebutyric acid N-hydroxysuccinimide ester (Pyr-NHS) is achieved to facilitate immobilization of H31L21 antibody. The effect of these modifications were studied with morphological, spectral and electrochemical techniques. The response of the biosensor was evaluated using differential pulse voltammetry (DPV). The data was acquired at a working potential of ~ 180 mV and a scan rate of 50 mV s−1. A low limit of detection (LOD) of 2.398 pM is achieved over a wide linear range from 11 pM to 55 nM. The biosensor exhibits excellent specificity over Aβ1–40 and ApoE ε4 interfering species. Thus, it provides a viable tool for electrochemical determination of Aβ1–42. Spiked human and mice plasmas were used for the successful validation of the sensing platform in bio-fluidic samples. The results obtained from mice plasma analysis concurred with the immunohistochemistry (IHC) and magnetic resonance imaging (MRI) data obtained from brain analysis.

Nanopore-Based, Label-Free, and Real-Time Monitoring Assay for DNA Methyltransferase Activity and Inhibition

2017 ◽  
Vol 89 (24) ◽  
pp. 13252-13260 ◽  
Author(s):  
Sana Rauf ◽  
Ling Zhang ◽  
Asghar Ali ◽  
Jalal Ahmad ◽  
Yang Liu ◽  
...  
Keyword(s):  
Real Time ◽  
Dna Methyltransferase ◽  
Label Free ◽  
Real Time Monitoring ◽  
Methyltransferase Activity
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