A thiol–thiosulfonate reaction providing a novel strategy for turn-on thiol sensing

2015 ◽  
Vol 51 (80) ◽  
pp. 14913-14916 ◽  
Author(s):  
Chunpo Ge ◽  
Hao Wang ◽  
Baoxin Zhang ◽  
Juan Yao ◽  
Xinming Li ◽  
...  
Keyword(s):  
Turn On ◽  
Novel Strategy ◽  
First Time

A thiosulfonate scaffold was applied to design selective and turn-on thiol probes for the first time.

Naked-Eye Detection of Hg(II) Ions by Visible Light-Induced Polymerization Initiated by a Hg(II)-Selective Photoredox Catalyst

2021 ◽  
Author(s):  
Hyungwook Kim ◽  
Young Jae Jung ◽  
Jungkyu K. Lee
Keyword(s):  
Visible Light ◽  
Signal Amplification ◽  
Eye Detection ◽  
Naked Eye ◽  
Turn On ◽  
Novel Strategy ◽  
Amplification Strategy ◽  
Naked Eye Detection

We developed a novel strategy for signal amplification strategy using a visible light-induced photopolymerization, initiated by a selective turn-on photoredox catalyst. As photoredox catalysts, fluorescein derivatives are able to initiate...

Engineering of a bioluminescent probe for imaging nitroxyl in live cells and mice

2019 ◽  
Vol 55 (12) ◽  
pp. 1758-1761 ◽  
Author(s):  
Jun-Bin Li ◽  
Qianqian Wang ◽  
Hong-Wen Liu ◽  
Xia Yin ◽  
Xiao-Xiao Hu ◽  
...  
Keyword(s):  
Live Cells ◽  
Turn On ◽  
First Time

A turn-on bioluminescent probe (BP-HNO) that is free of autofluorescence for bioimaging nitroxyl in live cells and mice is reported for the first time.

Antiapoptotic function of Bcl-2 in mast cells is dependent on its association with heat shock protein 90β

Blood ◽  
2006 ◽  
Vol 107 (4) ◽  
pp. 1413-1420 ◽  
Author(s):  
Cellina Cohen-Saidon ◽  
Irit Carmi ◽  
Avishai Keren ◽  
Ehud Razin
Keyword(s):  
Mast Cell ◽  
Rna Interference ◽  
Mast Cells ◽  
Heat Shock ◽  
Heat Shock Protein ◽  
Caspase 3 ◽  
Caspase 7 ◽  
Antiapoptotic Activity ◽  
Novel Strategy ◽  
First Time

In the present study, we demonstrated that the antiapoptotic function of Bcl-2 in mast cells is significantly dependent on its association with the heat shock protein 90β (Hsp90β). Dissociation of these 2 proteins inhibits the antiapoptotic activity of Bcl-2 by initiating the release of cytochrome c from mitochondria into cytosol and increasing the activity of caspase 3 and caspase 7, resulting in mast-cell apoptosis. The antiapoptotic activity of Bcl-2 was greatly affected by knocking-out specifically Hsp90β using the RNA interference approach. Thus, for the first time, it has been shown that Hsp90β might modulate the antiapoptotic activity of Bcl-2 at least in mast cells. These findings could have implications for a novel strategy of regulating apoptosis in patients with mastocytosis and other mast cell–associated diseases.

scholarly journals Aldo-Keto Reductase 1C3 Mediates Chemotherapy Resistance in Esophageal Adenocarcinoma via ROS Detoxification

Cancers ◽  
2021 ◽  
Vol 13 (10) ◽  
pp. 2403
Author(s):  
Chenghui Zhou ◽  
Zhefang Wang ◽  
Jiahui Li ◽  
Xiaolin Wu ◽  
Ningbo Fan ◽  
...  
Keyword(s):  
Esophageal Adenocarcinoma ◽  
Mrna Level ◽  
Chemotherapy Resistance ◽  
Poor Overall Survival ◽  
Akt Phosphorylation ◽  
Tumor Tissues ◽  
Novel Strategy ◽  
Induced Apoptosis ◽  
First Time ◽  
Eac Cells

Esophageal adenocarcinoma (EAC) is one of the most lethal malignancies, and limits promising treatments. AKR1C3 represents a therapeutic target to combat the resistance in many cancers. However, the molecular mechanism of AKR1C3 in the chemotherapy resistance of EAC is still unclear. We found that the mRNA level of AKR1C3 was higher in EAC tumor tissues, and that high AKR1C3 expression might be associated with poor overall survival of EAC patients. AKR1C3 overexpression decreased cell death induced by chemotherapeutics, while knockdown of AKR1C3 attenuated the effect. Furthermore, we found AKR1C3 was inversely correlated with ROS production. Antioxidant NAC rescued chemotherapy-induced apoptosis in AKR1C3 knockdown cells, while the GSH biosynthesis inhibitor BSO reversed a protective effect of AKR1C3 against chemotherapy. AKT phosphorylation was regulated by AKR1C3 and might be responsible for eliminating over-produced ROS in EAC cells. Intracellular GSH levels were modulated by AKR1C3 and the inhibition of AKT could reduce GSH level in EAC cells. Here, we reported for the first time that AKR1C3 renders chemotherapy resistance through controlling ROS levels via AKT signaling in EAC cells. Targeting AKR1C3 may represent a novel strategy to sensitize EAC cells to conventional chemotherapy.

scholarly journals A red-emissive antibody–AIEgen conjugate for turn-on and wash-free imaging of specific cancer cells

2017 ◽  
Vol 8 (10) ◽  
pp. 7014-7024 ◽  
Author(s):  
Xiujuan Shi ◽  
Chris Y. Y. Yu ◽  
Huifang Su ◽  
Ryan T. K. Kwok ◽  
Meijuan Jiang ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Cancer Cells ◽  
Turn On ◽  
Specific Cancer ◽  
First Time

For the first time, an AIEgen-conjugated monoclonal antibody is designed for “turn-on” and “wash-free” imaging of EGFR-overexpressed cancer cells.

scholarly journals Acylation derivatization based LC-MS analysis of 25-hydroxyvitamin D from finger-prick blood

2019 ◽  
Vol 60 (5) ◽  
pp. 1058-1064 ◽  
Author(s):  
Juan Le ◽  
Teng-Fei Yuan ◽  
Jia-Qing Geng ◽  
Shao-Ting Wang ◽  
Yan Li ◽  
...  
Keyword(s):  
Vitamin D ◽  
Vitamin D Supplementation ◽  
Diels Alder ◽  
Clinical Value ◽  
Hydroxyvitamin D ◽  
Optimal Outcomes ◽  
Finger Prick ◽  
25 Hydroxyvitamin D ◽  
Novel Strategy ◽  
First Time

Vitamin D metabolite analysis possessed significant clinical value for the pediatric department. However, invasive venipuncture sampling and high blood consumption inflicted much suffering on patients. For alleviation, we carried out a LC-MS method for 25-hydroxyvitamin D quantification in only 3 μl of plasma from the considerably less invasive finger-prick blood samples. To improve sensitivity, acylation on C3-hydroxyl (by isonicotinoyl chloride) rather than Diels-Alder adduction on s-cis-diene structure was for the very first time introduced into vitamin D metabolite derivatization. Compared with the existing derivatization approaches, this novel strategy not only prevented isomer interference, but also exhibited higher reacting throughput. For certification, the methodology was systematically validated and showed satisfying consistency with SRM927a. During clinical application, we found a convincing correlation between 25-hydroxyvitamin D and indirect/total bilirubin in jaundiced newborns. Such an observation indicated that vitamin D supplementation may help to achieve optimal outcomes in neonatal jaundice.

scholarly journals Tetrazine as a general phototrigger to turn on fluorophores

2020 ◽  
Vol 11 (17) ◽  
pp. 4410-4415 ◽  
Author(s):  
Axel Loredo ◽  
Juan Tang ◽  
Lushun Wang ◽  
Kuan-Lin Wu ◽  
Zane Peng ◽  
...  
Keyword(s):  
Turn On ◽  
First Time

Tetrazine was demonstrated for the first time as a general phototrigger to design photoactivatable fluorophore probes.

Pyrene appended thymine derivative for selective turn-on fluorescence sensing of uric acid in live cells

RSC Advances ◽  
2016 ◽  
Vol 6 (71) ◽  
pp. 66774-66778 ◽  
Author(s):  
Prithidipa Sahoo ◽  
Himadri Sekhar Sarkar ◽  
Sujoy Das ◽  
Kalipada Maiti ◽  
Md Raihan Uddin ◽  
...  
Keyword(s):  
Uric Acid ◽  
Fluorescent Probe ◽  
High Sensitivity ◽  
Living Cells ◽  
Live Cells ◽  
Fluorescence Sensing ◽  
Turn On ◽  
Sensitivity And Selectivity ◽  
First Time

A new ‘turn-ON’ fluorescent probe, pyrene appended thymine acetamide (PTA), with high sensitivity and selectivity for the detection of uric acid (UA) was developed and first time imaging of uric acid in living cells in water was achieved.

scholarly journals Synergistic Effects of Adding a Substrate and an Organic Reductant for Stimulating the Bioremediation of 4-chloronitrobenzene-contaminated Soil

2020 ◽  
Author(s):  
Tian Li ◽  
Ping Zhang ◽  
Kun Qian ◽  
Tian.C Zhang
Keyword(s):  
Contaminated Soil ◽  
Organic Nitrogen ◽  
Synergistic Effects ◽  
Sufficient Information ◽  
Batch Reactors ◽  
Future Studies ◽  
Organic Nitrogen Source ◽  
Novel Strategy ◽  
First Time ◽  
Soil Batch

Abstract Background:Soil contaminated with 4-chloronitrobenzene (4NCB) is resistant to microbial degradation due to the electron-withdrawing properties of the nitro and chloro groups in 4NCB. Currently, sufficient information is not available on how to use biostimulation strategies to enhance the bioremediation of 4NCB-contaminated soil.Results:In the present study, a novel strategy was developed by utilizing the synergistic effects of adding an organic reductant (ascorbic acid, VC) and an organic nitrogen source (peptone) to stimulate the biodegradation of 4NCB-contaminated soil. Using this strategy, the bioremediation of 1 g-4NCB/ kg-1 soil could be completed within 8 days in soil batch reactors. Furthermore, the study discovered two 4NCB cometabolic intermediates in the soil reactors added with peptone and VC, and for the first time, 4NCB was transformed to 4-chlorofromanilide.Conclusion:The proposed strategy is promising because it is highly efficient, easy to control and involves a non-toxic, environmentally friendly substrate/reductant.). Finally, this approach warrants future studies to extend its applications to soils contaminated with other nitroaromatic compounds.

Proof of critical speed of high-speed rail underlain by stratified media

2020 ◽  
Vol 476 (2240) ◽  
pp. 20200083
Author(s):  
Eduardo Kausel ◽  
José Estaire ◽  
Inés Crespo-Chacón
Keyword(s):  
Twentieth Century ◽  
Upper Bound ◽  
High Speed ◽  
Critical Speed ◽  
Complete System ◽  
Stratified Media ◽  
High Speed Rail ◽  
Turn On ◽  
Mathematical Explanations ◽  
First Time

As is well known, the safe travel velocity of high-speed rail must remain below a well-defined limit commonly referred to as the critical speed . This upper bound depends, in turn, on the speed at which waves propagate in the ground. But as models for trains in motion have increased in complexity, over the years the concept of critical speed has grown in obscurity. It was not until late in the twentieth century that it was realized that the critical speed was somehow connected to the so-called dispersion spectrum for the complete system, but until now, the justification for its application in practice has remained empirical and lacking in rigorous mathematical explanations. This task is taken up in this article, where for the first time a most general proof is provided for the problem at hand that is applicable to any layered soil configuration when one such system is subjected to one or more loads in motion.

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