Engineering of a bioluminescent probe for imaging nitroxyl in live cells and mice

2019 ◽  
Vol 55 (12) ◽  
pp. 1758-1761 ◽  
Author(s):  
Jun-Bin Li ◽  
Qianqian Wang ◽  
Hong-Wen Liu ◽  
Xia Yin ◽  
Xiao-Xiao Hu ◽  
...  
Keyword(s):  
Live Cells ◽  
Turn On ◽  
First Time

A turn-on bioluminescent probe (BP-HNO) that is free of autofluorescence for bioimaging nitroxyl in live cells and mice is reported for the first time.

Pyrene appended thymine derivative for selective turn-on fluorescence sensing of uric acid in live cells

RSC Advances ◽  
2016 ◽  
Vol 6 (71) ◽  
pp. 66774-66778 ◽  
Author(s):  
Prithidipa Sahoo ◽  
Himadri Sekhar Sarkar ◽  
Sujoy Das ◽  
Kalipada Maiti ◽  
Md Raihan Uddin ◽  
...  
Keyword(s):  
Uric Acid ◽  
Fluorescent Probe ◽  
High Sensitivity ◽  
Living Cells ◽  
Live Cells ◽  
Fluorescence Sensing ◽  
Turn On ◽  
Sensitivity And Selectivity ◽  
First Time

A new ‘turn-ON’ fluorescent probe, pyrene appended thymine acetamide (PTA), with high sensitivity and selectivity for the detection of uric acid (UA) was developed and first time imaging of uric acid in living cells in water was achieved.

A bioluminescent probe for imaging endogenous hydrogen polysulfides in live cells and a murine model of bacterial infection

2019 ◽  
Vol 55 (31) ◽  
pp. 4487-4490 ◽  
Author(s):  
Jun-Bin Li ◽  
Qianqian Wang ◽  
Hong-Wen Liu ◽  
Lin Yuan ◽  
Xiao-Bing Zhang
Keyword(s):  
Bacterial Infection ◽  
Murine Model ◽  
Live Cells ◽  
Turn On ◽  
First Time

A turn-on bioluminescent probe (BP-PS) for bioimaging endogenous H2Sn in live cells and a murine model of bacterial infection was, for the first time, reported.

Turn-on fluorescent probe for dopamine detection in solutions and live cells based on in situ formation of aminosilane-functionalized carbon dots

2021 ◽  
Vol 1157 ◽  
pp. 338394
Author(s):  
Xiao-Yue Tang ◽  
Yi-Ming Liu ◽  
Xiao-Lin Bai ◽  
Hao Yuan ◽  
Yi-Kao Hu ◽  
...  
Keyword(s):  
Fluorescent Probe ◽  
Carbon Dots ◽  
Live Cells ◽  
Turn On ◽  
Dopamine Detection ◽  
In Situ Formation

A single probe to sense Al(iii) colorimetrically and Cd(ii) by turn-on fluorescence in physiological conditions and live cells, corroborated by X-ray crystallographic and theoretical studies

2015 ◽  
Vol 44 (9) ◽  
pp. 4123-4132 ◽  
Author(s):  
Chirantan Kar ◽  
Soham Samanta ◽  
Sudeep Goswami ◽  
Aiyagari Ramesh ◽  
Gopal Das
Keyword(s):  
Live Cell Imaging ◽  
Cell Imaging ◽  
Selective Recognition ◽  
Live Cells ◽  
Paper Strip ◽  
Theoretical Studies ◽  
X Ray ◽  
Physiological Conditions ◽  
Turn On ◽  
Single Probe

Selective recognition of Al3+and Cd2+by UV-Vis and fluorescence based techniques using a cinnamaldehyde functionalized conjugated ligand, and its applications in paper strip and live cell imaging.

scholarly journals Live-Cell Imaging of Caspase Activation for High-Content Screening

2009 ◽  
Vol 14 (8) ◽  
pp. 956-969 ◽  
Author(s):  
Christophe Antczak ◽  
Toshimitsu Takagi ◽  
Christina N. Ramirez ◽  
Constantin Radu ◽  
Hakim Djaballah
Keyword(s):  
Cell Death ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Exposure Period ◽  
Live Cells ◽  
Assay Method ◽  
Caspase Activation ◽  
Fluorogenic Substrate ◽  
Automated Imaging ◽  
First Time

Caspases are central to the execution of programmed cell death, and their activation constitutes the biochemical hallmark of apoptosis. In this article, the authors report the successful adaptation of a high-content assay method using the DEVDNucView488™ fluorogenic substrate, and for the first time, they show caspase activation in live cells induced by either drugs or siRNA. The fluorogenic substrate was found to be nontoxic over an exposure period of several days, during which the authors demonstrate automated imaging and quantification of caspase activation of the same cell population as a function of time. Overexpression of the antiapoptotic protein Bcl-XL, alone or in combination with the inhibitor Z-VAD-FMK, attenuated caspase activation in HeLa cells exposed to doxorubicin, etoposide, or cell death siRNA. This method was further validated against 2 well-characterized NSCLC cell lines reported to be sensitive (H3255) or refractory (H2030) to erlotinib, where the authors show a differential time-dependent activation was observed for H3255 and no significant changes in H2030, consistent with their respective chemosensitivity profile. In summary, the results demonstrate the feasibility of using this newly adapted and validated high-content assay to screen chemical or RNAi libraries for the identification of previously uncovered enhancers and suppressors of the apoptotic machinery in live cells. ( Journal of Biomolecular Screening 2009:956-969)

A naphthalimide based PET probe with Fe3+ selective detection ability: theoretical and experimental study

The Analyst ◽  
2014 ◽  
Vol 139 (24) ◽  
pp. 6352-6356 ◽  
Author(s):  
Narendra Reddy Chereddy ◽  
M. V. Niladri Raju ◽  
Peethani Nagaraju ◽  
Venkat Raghavan Krishnaswamy ◽  
Purna Sai Korrapati ◽  
...  
Keyword(s):  
Experimental Study ◽  
Live Cells ◽  
Selective Detection ◽  
Aqueous Samples ◽  
Detection Ability ◽  
Turn On ◽  
Fluorescence Turn On

A naphthalimide based Fe3+ selective fluorescence ‘turn-on’ probe that operates based on a PET mechanism has been synthesized, and its application in the detection of Fe3+ ions in aqueous samples and in live cells is explored.

A highly selective and sensitive fluorescence “turn-on” probe for Ag+ in aqueous solution and live cells

2012 ◽  
Vol 41 (24) ◽  
pp. 7212 ◽  
Author(s):  
Lin Xu ◽  
Yufang Xu ◽  
Weiping Zhu ◽  
Chunmei Yang ◽  
Le Han ◽  
...  
Keyword(s):  
Aqueous Solution ◽  
Live Cells ◽  
Turn On ◽  
Fluorescence Turn On

scholarly journals A red-emissive antibody–AIEgen conjugate for turn-on and wash-free imaging of specific cancer cells

2017 ◽  
Vol 8 (10) ◽  
pp. 7014-7024 ◽  
Author(s):  
Xiujuan Shi ◽  
Chris Y. Y. Yu ◽  
Huifang Su ◽  
Ryan T. K. Kwok ◽  
Meijuan Jiang ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Cancer Cells ◽  
Turn On ◽  
Specific Cancer ◽  
First Time

For the first time, an AIEgen-conjugated monoclonal antibody is designed for “turn-on” and “wash-free” imaging of EGFR-overexpressed cancer cells.

scholarly journals Interaction between Acetylated MyoD and the Bromodomain of CBP and/or p300

2001 ◽  
Vol 21 (16) ◽  
pp. 5312-5320 ◽  
Author(s):  
Anna Polesskaya ◽  
Irina Naguibneva ◽  
Arnaud Duquet ◽  
Eyal Bengal ◽  
Philippe Robin ◽  
...  
Keyword(s):  
Transcriptional Activation ◽  
Protein Function ◽  
Live Cells ◽  
Regulation Of Transcription ◽  
Muscle Creatine Kinase ◽  
Nonhistone Protein ◽  
Myogenic Factor ◽  
First Time

ABSTRACT Acetylation is emerging as a posttranslational modification of nuclear proteins that is essential to the regulation of transcription and that modifies transcription factor affinity for binding sites on DNA, stability, and/or nuclear localization. Here, we present both in vitro and in vivo evidence that acetylation increases the affinity of myogenic factor MyoD for acetyltransferases CBP and p300. In myogenic cells, the fraction of endogenous MyoD that is acetylated was found associated with CBP or p300. In vitro, the interaction between MyoD and CBP was more resistant to high salt concentrations and was detected with lower doses of MyoD when MyoD was acetylated. Interestingly, an analysis of CBP mutants revealed that the interaction with acetylated MyoD involves the bromodomain of CBP. In live cells, MyoD mutants that cannot be acetylated did not associate with CBP or p300 and were strongly impaired in their ability to cooperate with CBP for transcriptional activation of a muscle creatine kinase-luciferase construct. Taken together, our data suggest a new mechanism for activation of protein function by acetylation and demonstrate for the first time an acetylation-dependent interaction between the bromodomain of CBP and a nonhistone protein.

Tracking the Formation and Degradation of Fatty-Acid-Accumulated Mitochondria Using Label-Free Chemical Imaging

2020 ◽  
Author(s):  
Chi Zhang ◽  
Stephen Boppart
Keyword(s):  
Fatty Acid ◽  
Structural Features ◽  
Chemical Imaging ◽  
Live Cells ◽  
Label Free ◽  
Dynamic Changes ◽  
Regulatory Processes ◽  
Transport Chain ◽  
Anti Stokes ◽  
First Time

Abstract The mitochondrion is one of the key organelles for maintaining cellular homeostasis. External environmental stimuli and internal regulatory processes alter the metabolism and functions of mitochondria. To understand these activities of mitochondria, it is critical to probe the key metabolic molecules inside these organelles. In this study, we used label-free chemical imaging modalities including coherent anti-Stokes Raman scattering and multiphoton-excited autofluorescence to study the mitochondrial activities in living cancer cells. We found that hypothermia exposure tends to induce fatty-acid (FA) accumulation in some mitochondria of MIAPaCa-2 cells. Autofluorescence images show that the FA-accumulated mitochondria also have abnormal NADH and FAD metabolism, likely induced by the dysfunction of the electron transport chain. We also found that when the cells were re-warmed to physiological temperature after a period of hypothermia, the FA-accumulated mitochondria changed their structural features, likely caused by the mitophagy process. To the best of our knowledge, this is the first time that FA accumulation in mitochondria was observed in live cells. Our research also demonstrates that multimodal label-free chemical imaging is an attractive tool to discover abnormal functions of mitochondria at the single-organelle level and can be used to quantify the dynamic changes of this organelle under perturbative conditions.

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