Highly sensitive turn-on biosensors by regulating fluorescent dye assembly on liposome surfaces

2015 ◽  
Vol 51 (50) ◽  
pp. 10229-10232 ◽  
Author(s):  
Sungbaek Seo ◽  
Min Sang Kwon ◽  
Andrew W. Phillips ◽  
Deokwon Seo ◽  
Jinsang Kim
Keyword(s):  
Fluorescent Dye ◽  
Specific Interactions ◽  
Turn On ◽  
Sensing Platform ◽  
Highly Sensitive ◽  
Emission Quenching ◽  
Phospholipid Liposome ◽  
Fluorescence Dyes ◽  
Target Analyte

Herein, we developed a turn-on type liposome-based sensing platform by assembling fluorescence dyes to form H-type aggregation with emission quenching on phospholipid-liposome surfaces. Rationally devised specific interactions between the phospholipid and a target analyte effectively release the fluorescence dyes from H-aggregate, producing a turn-on signal.

A phenanthrene[9,10-d]imidazole-phenol-based fluorescent probe combined ESIPT and AIE for “turn-on” detection of Cu2+ with a green-emitting and improved stokes shift and its application

2021 ◽  
Author(s):  
Jiahui Du ◽  
Bing Zhao ◽  
Wei Kan ◽  
Haochun Yin ◽  
Tianshu Song ◽  
...  
Keyword(s):  
Fluorescent Probe ◽  
Stokes Shift ◽  
Fluorescent Sensors ◽  
Turn On ◽  
Highly Sensitive

Development of highly sensitive and selective fluorescent sensors toward Cu2+ has gained considerable attention in view of its application of environmental and biological fields. However, the strategy of sensing by...

A highly-sensitive “turn on” probe based on coumarin β-diketone for hydrazine detection in PBS and living cells

2021 ◽  
Vol 252 ◽  
pp. 119510
Author(s):  
Rui Chen ◽  
Guang-Jin Shi ◽  
Jia-Jia Wang ◽  
Hai-Feng Qin ◽  
Qi Zhang ◽  
...  
Keyword(s):  
Living Cells ◽  
Turn On ◽  
Highly Sensitive

A Highly Sensitive and Label-Free Microbead-Based ‘Turn-On’ Colorimetric Sensor for the Detection of Mercury(II) in Urine Using a Peroxidase-Like Split G-Quadruplex–Hemin DNAzyme

2018 ◽  
Vol 71 (12) ◽  
pp. 945
Author(s):  
Xin Fu ◽  
He Zhang ◽  
Jie Zhang ◽  
Shi-Tong Wen ◽  
Xing-Cheng Deng
Keyword(s):  
Standard Deviation ◽  
Base Pair ◽  
Limit Of Detection ◽  
Label Free ◽  
Turn On ◽  
Highly Sensitive ◽  
Relative Standard ◽  
G Quadruplex ◽  
Catalytically Active ◽  
Partial Cdna

A highly sensitive and label-free microbead-based ‘turn-on’ assay was developed for the detection of Hg2+ in urine based on the Hg2+-mediated formation of intermolecular split G-quadruplex–hemin DNAzymes. In the presence of Hg2+, T–T mismatches between the two partial cDNA strands were stabilized by a T–Hg2+–T base pair, and can cause the G-rich sequences of the two oligonucleotides to associate to form a split G-quadruplex which is able to bind hemin to form the catalytically active G-quadruplex–hemin DNAzyme. This microbead-based ‘turn-on’ process allows the detection of Hg2+ in urine samples at concentrations as low as 0.5 pM. The relative standard deviation and recovery are 1.2–3.9 and 98.7–103.2%, respectively. The remarkable sensitivity for Hg2+ is mainly attributed to the enhanced mass transport ability that is inherent in homogeneous microbead-based assays. Compared with previous developments of intermolecular split G-quardruplex–hemin DNAzymes for the homogeneous detection of Hg2+ (the limit of detection was 19nM), a signal enhancement of ~1000 times is obtained when such an assay is performed on the surface of microbeads.

A highly sensitive turn-on ratiometric luminescent probe based on postsynthetic modification of Tb3+@Cu-MOF for H2S detection

2017 ◽  
Vol 5 (38) ◽  
pp. 9943-9951 ◽  
Author(s):  
Xubin Zheng ◽  
Ruiqing Fan ◽  
Yang Song ◽  
Ani Wang ◽  
Kai Xing ◽  
...  
Keyword(s):  
High Sensitivity ◽  
Luminescent Probe ◽  
Turn On ◽  
Postsynthetic Modification ◽  
Highly Sensitive ◽  
Sensitivity And Selectivity ◽  
H2s Detection

A novel probe, designed and prepared through PSM of Cu-MOFs and Tb3+ions, shows high sensitivity and selectivity for H2S.

Ti mesh loaded with Ag “nanobosk”: A highly sensitive Raman sensing platform for trace norfloxacin in water

2019 ◽  
Vol 283 ◽  
pp. 163-171 ◽  
Author(s):  
Xiaomeng Dou ◽  
Weiming Hao ◽  
Xiangqing Li ◽  
Lixia Qin ◽  
Shi-Zhao Kang
Keyword(s):  
Sensing Platform ◽  
Highly Sensitive ◽  
Ti Mesh

Integrating dye-intercalated DNA dendrimers with electrospun nanofibers: a new fluorescent sensing platform for nucleic acids, proteins, and cells

2015 ◽  
Vol 3 (17) ◽  
pp. 3541-3547 ◽  
Author(s):  
Huaming Wang ◽  
Wei Tang ◽  
Hejia Wei ◽  
Yan Zhao ◽  
Shichao Hu ◽  
...  
Keyword(s):  
Nucleic Acids ◽  
Related Species ◽  
Electrospun Nanofibers ◽  
Fluorescent Dye ◽  
Fluorescent Sensing ◽  
Sensing Platform

A fluorescent dye-intercalated DNA dendrimer probe was integrated with electrospun nanofibers to create an amplified sensing platform for disease-related species.

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