Utilizing stem cells for three-dimensional neural tissue engineering

2016 ◽  
Vol 4 (5) ◽  
pp. 768-784 ◽  
Author(s):  
Stephanie Knowlton ◽  
Yongku Cho ◽  
Xue-Jun Li ◽  
Ali Khademhosseini ◽  
Savas Tasoglu
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Three Dimensional ◽  
Neural Tissue ◽  
Disease Models ◽  
Neural Tissue Engineering

Three-dimensional neural tissue engineering has significantly advanced the development of neural disease models and replacement tissues for patients by leveraging the unique capabilities of stem cells.

scholarly journals Advancing fabrication and properties of three-dimensional graphene–alginate scaffolds for application in neural tissue engineering

RSC Advances ◽  
2019 ◽  
Vol 9 (63) ◽  
pp. 36838-36848
Author(s):  
Negar Mansouri ◽  
Said F. Al-Sarawi ◽  
Jagan Mazumdar ◽  
Dusan Losic
Keyword(s):  
Tissue Engineering ◽  
Three Dimensional ◽  
Neural Tissue ◽  
Fabrication Method ◽  
Neural Tissue Engineering ◽  
Composite Scaffolds ◽  
3D Graphene

In this study, a bio-fabrication method has been developed for the preparation of 3D graphene–alginate composite scaffolds with great potential for neural tissue engineering.

Stem cells and biomaterial interactions for neural tissue engineering models: A matter of mechanotransduction

2010 ◽  
Vol 150 ◽  
pp. 469-470
Author(s):  
D. D’Angelo ◽  
I. Armentano ◽  
R. Tiribuzi ◽  
S. Mattioli ◽  
U. Reale ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Neural Tissue ◽  
Neural Tissue Engineering ◽  
Engineering Models

scholarly journals Effects of Graphene-Based Materials on the Behavior of Neural Stem Cells

2020 ◽  
Vol 2020 ◽  
pp. 1-16 ◽  
Author(s):  
Yan Zhang ◽  
Shu Wang ◽  
Ping Yang
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Neural Stem Cells ◽  
Transverse Myelitis ◽  
Neural Tissue ◽  
Research Field ◽  
Benign Tumors ◽  
Neural Tissue Engineering ◽  
Coating Materials ◽  
Differentiation And Proliferation

Neural tissue engineering is a research field aimed at rebuilding neurological defects resulting from severe trauma, vascular impairment, syringomyelia, spinal stenosis, malignant and benign tumors, or transverse myelitis. Of particular interest, neural stem cells (NSCs) and the effective differentiation and proliferation thereof are attractive research areas that have yielded widespread utility for implants or neural scaffold materials. Graphene and its derivatives have more effective and efficient physical, chemical, and biological abilities than other nanomaterials, and may act as new coating materials to promote neuronal proliferation and differentiation. Therefore, here, we review the recent progress of studies that examine the effect of graphene-based materials on NSCs. We specifically review how graphene and its derivatives influence NSC adhesion, differentiation, and proliferation. We also discuss the risks of graphene-based materials, including their anti-inflammatory effects, in the realm of neural tissue engineering as well as current challenges facing the field today.

In vivo neural tissue engineering using adipose-derived mesenchymal stem cells and fibrin matrix

2021 ◽  
pp. 1-15
Author(s):  
Krishnapriya Chandrababu ◽  
Harikrishnan Vijayakumar Sreelatha ◽  
Tara Sudhadevi ◽  
Arya Anil ◽  
Sabareeswaran Arumugam ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Neural Tissue ◽  
Neural Tissue Engineering ◽  
Fibrin Matrix

Three-Dimensional Collagen Gel Networks for Neural Stem Cell-Based Neural Tissue Engineering

2005 ◽  
Vol 227 (1) ◽  
pp. 327-334 ◽  
Author(s):  
Wu Ma ◽  
Silvia Chen ◽  
Wendy Fitzgerald ◽  
Dragan Maric ◽  
Hsingch J. Lin ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cell ◽  
Neural Stem Cell ◽  
Three Dimensional ◽  
Collagen Gel ◽  
Neural Tissue ◽  
Neural Tissue Engineering

scholarly journals Pyrrole Plasma Polymer-Coated Electrospun Scaffolds for Neural Tissue Engineering

Polymers ◽  
2021 ◽  
Vol 13 (22) ◽  
pp. 3876
Author(s):  
Diana María Osorio-Londoño ◽  
José Rafael Godínez-Fernández ◽  
Ma. Cristina Acosta-García ◽  
Juan Morales-Corona ◽  
Roberto Olayo-González ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Colorimetric Assay ◽  
Three Dimensional ◽  
Neural Tissue ◽  
Plasma Polymer ◽  
Neural Cells ◽  
Neural Tissue Engineering ◽  
Microscopy Imaging ◽  
Mtt Colorimetric Assay ◽  
Cell Structures

Promising strategies for neural tissue engineering are based on the use of three-dimensional substrates for cell anchorage and tissue development. In this work, fibrillar scaffolds composed of electrospun randomly- and aligned-oriented fibers coated with plasma synthesized pyrrole polymer, doped and undoped with iodine, were fabricated and characterized. Infrared spectroscopy, thermogravimetric analysis, and X-ray diffraction analysis revealed the functional groups and molecular integration of each scaffold, as well as the effect of plasma polymer synthesis on crystallinity. Scanning microscopy imaging demonstrated the porous fibrillar micrometric structure of the scaffolds, which afforded adhesion, infiltration, and survival for the neural cells. Orientation analysis of electron microscope images confirmed the elongation of neurite-like cell structures elicited by undoped plasma pyrrole polymer-coated aligned scaffolds, without any biochemical stimuli. The MTT colorimetric assay validated the biocompatibility of the fabricated composite materials, and further evidenced plasma pyrrole polymer-coated aligned scaffolds as permissive substrates for the support of neural cells. These results suggest plasma synthesized pyrrole polymer-coated aligned scaffolds are promising materials for tissue engineering applications.

scholarly journals Electrical Stimulation Promotes Stem Cell Neural Differentiation in Tissue Engineering

2021 ◽  
Vol 2021 ◽  
pp. 1-14
Author(s):  
Hong Cheng ◽  
Yan Huang ◽  
Hangqi Yue ◽  
Yubo Fan
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Stem Cell ◽  
Electrical Stimulation ◽  
Neural Differentiation ◽  
Biological Effects ◽  
Neural Tissue ◽  
Neural Regeneration ◽  
Neural Tissue Engineering ◽  
Conductive Materials

Nerve injuries and neurodegenerative disorders remain serious challenges, owing to the poor treatment outcomes of in situ neural stem cell regeneration. The most promising treatment for such injuries and disorders is stem cell-based therapies, but there remain obstacles in controlling the differentiation of stem cells into fully functional neuronal cells. Various biochemical and physical approaches have been explored to improve stem cell-based neural tissue engineering, among which electrical stimulation has been validated as a promising one both in vitro and in vivo. Here, we summarize the most basic waveforms of electrical stimulation and the conductive materials used for the fabrication of electroactive substrates or scaffolds in neural tissue engineering. Various intensities and patterns of electrical current result in different biological effects, such as enhancing the proliferation, migration, and differentiation of stem cells into neural cells. Moreover, conductive materials can be used in delivering electrical stimulation to manipulate the migration and differentiation of stem cells and the outgrowth of neurites on two- and three-dimensional scaffolds. Finally, we also discuss the possible mechanisms in enhancing stem cell neural differentiation using electrical stimulation. We believe that stem cell-based therapies using biocompatible conductive scaffolds under electrical stimulation and biochemical induction are promising for neural regeneration.

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