scholarly journals Electrical Stimulation Promotes Stem Cell Neural Differentiation in Tissue Engineering

2021 ◽  
Vol 2021 ◽  
pp. 1-14
Author(s):  
Hong Cheng ◽  
Yan Huang ◽  
Hangqi Yue ◽  
Yubo Fan
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Stem Cell ◽  
Electrical Stimulation ◽  
Neural Differentiation ◽  
Biological Effects ◽  
Neural Tissue ◽  
Neural Regeneration ◽  
Neural Tissue Engineering ◽  
Conductive Materials

Nerve injuries and neurodegenerative disorders remain serious challenges, owing to the poor treatment outcomes of in situ neural stem cell regeneration. The most promising treatment for such injuries and disorders is stem cell-based therapies, but there remain obstacles in controlling the differentiation of stem cells into fully functional neuronal cells. Various biochemical and physical approaches have been explored to improve stem cell-based neural tissue engineering, among which electrical stimulation has been validated as a promising one both in vitro and in vivo. Here, we summarize the most basic waveforms of electrical stimulation and the conductive materials used for the fabrication of electroactive substrates or scaffolds in neural tissue engineering. Various intensities and patterns of electrical current result in different biological effects, such as enhancing the proliferation, migration, and differentiation of stem cells into neural cells. Moreover, conductive materials can be used in delivering electrical stimulation to manipulate the migration and differentiation of stem cells and the outgrowth of neurites on two- and three-dimensional scaffolds. Finally, we also discuss the possible mechanisms in enhancing stem cell neural differentiation using electrical stimulation. We believe that stem cell-based therapies using biocompatible conductive scaffolds under electrical stimulation and biochemical induction are promising for neural regeneration.

scholarly journals Biodegradable and Biocompatible Graphene-based Scaffolds for Functional Neural Tissue Engineering: A Strategy Approach Using Dental Pulp Stem Cells and Biomaterials

2021 ◽  
Author(s):  
Negar Mansouri ◽  
Said Al-Sarawi ◽  
Dusan Losic ◽  
Jagan Mazumdar ◽  
Jillian Clark ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Cell Viability ◽  
Dental Pulp ◽  
Biological Effects ◽  
Neural Tissue ◽  
Dental Pulp Stem Cells ◽  
Neural Tissue Engineering ◽  
3D Scaffolds ◽  
Serum Free

AbstractNeural tissue engineering aims to restore function of nervous system tissues using biocompatible cell-seeded scaffolds. Graphene-based scaffolds combined with stem cells deserve special attention to enhance tissue regeneration in a controlled manner. However, it is believed that minor changes in scaffold biomaterial com-position, internal porous structure, and physicochemical properties can impact cellular growth and adhesion. The current work aims to investigate in vitro biological effects of 3D graphene oxide (GO)/sodium alginate (GOSA) and reduced GOSA (RGOSA) scaffolds on dental pulp stem cells (DPSCs) in terms of cell viability and cytotoxicity. Herein, the effects of the 3D scaffolds, coating conditions, and serum supplementation on DPSCs functions are explored extensively. Biodegradation analysis revealed that addition of GO enhanced the degradation rate of composite scaffolds. Compared to the 2D surface, the cell viability of 3D scaffolds was higher (p <0.0001), highlighting the optimal initial cell adhesion to the scaffold surface and cell migration through pores. Moreover, the cytotoxicity study indicated that the incorporation of graphene supported higher DPSCs viability. It is also shown that when the mean pore size of scaffold increases, DPSCs activity decreases. In terms of coating conditions, poly-l-lysine (PLL) was the most robust coating reagent that improved cell-scaffold adherence and DPSCs metabolism activity. The cytotoxicity of GO-based scaffolds showed that DPSCs can be seeded in serum-free media without cytotoxic effects. This is critical for human translation as cellular transplants are typically serum-free. These findings suggest that proposed 3D GO-based scaffolds have favourable effects on the biological responses of DPSCs.

scholarly journals Biodegradable and Biocompatible Graphene-based Scaffolds for Functional Neural Tissue Engineering: A Strategy Approach Using Dental Pulp Stem Cells and Biomaterials

2021 ◽  
Author(s):  
Negar Mansouri ◽  
Said Al-Sarawi ◽  
Dusan Losic ◽  
Jagan Mazumdar ◽  
Jillian Clark ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Cell Viability ◽  
Dental Pulp ◽  
Biological Effects ◽  
Neural Tissue ◽  
Dental Pulp Stem Cells ◽  
Neural Tissue Engineering ◽  
3D Scaffolds ◽  
Serum Free

Neural tissue engineering aims to restore function of nervous system tissues using biocompatible cell-seeded scaffolds. Graphene-based scaffolds combined with stem cells deserve special attention to enhance tissue regeneration in a controlled manner. However, it is believed that minor changes in scaffold biomaterial composition, internal porous structure, and physicochemical properties can impact cellular growth and adhesion. The current work aims to investigate in vitro biological effects of 3D graphene oxide (GO)/sodium alginate (GOSA) and reduced GOSA (RGOSA) scaffolds on dental pulp stem cells (DPSCs) in terms of cell viability and cytotoxicity. Herein, the effects of the 3D scaffolds, coating conditions, and serum supplementation on DPSCs functions are explored extensively. Biodegradation analysis revealed that addition of GO enhanced the degradation rate of composite scaffolds. Compared to the 2D surface, the cell viability of 3D scaffolds was higher (p <0.0001), highlighting the optimal initial cell adhesion to the scaffold surface and cell migration through pores. Moreover, the cytotoxicity study indicated that the incorporation of graphene supported higher DPSCs viability. It is also shown that when the mean pore size of scaffold increases, DPSCs activity decreases. In terms of coating conditions, poly-l-lysine (PLL) was the most robust coating reagent that improved cell-scaffold adherence and DPSCs metabolism activity. The cytotoxicity of GO-based scaffolds showed that DPSCs can be seeded in serum-free media without cytotoxic effects. This is critical for human translation as cellular transplants are typically serum-free. These findings suggest that proposed 3D GO-based scaffolds have favourable effects on the biological responses of DPSCs.

scholarly journals Graphene-Based Nanocomposites for Neural Tissue Engineering

Molecules ◽  
2019 ◽  
Vol 24 (4) ◽  
pp. 658 ◽  
Author(s):  
Ho Bei ◽  
Yuhe Yang ◽  
Qiang Zhang ◽  
Yu Tian ◽  
Xiaoming Luo ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cell ◽  
Neural Stem Cell ◽  
High Surface ◽  
High Surface Area ◽  
Neural Tissue ◽  
Stem Cell Differentiation ◽  
Neural Regeneration ◽  
Neural Tissue Engineering ◽  
Graphene Nanocomposites

Graphene has made significant contributions to neural tissue engineering due to its electrical conductivity, biocompatibility, mechanical strength, and high surface area. However, it demonstrates a lack of biological and chemical cues. Also, it may cause potential damage to the host body, limiting its achievement of efficient construction of neural tissues. Recently, there has been an increasing number of studies showing that combining graphene with other materials to form nano-composites can provide exceptional platforms for both stimulating neural stem cell adhesion, proliferation, differentiation and neural regeneration. This suggests that graphene nanocomposites are greatly beneficial in neural regenerative medicine. In this mini review, we will discuss the application of graphene nanocomposites in neural tissue engineering and their limitations, through their effect on neural stem cell differentiation and constructs for neural regeneration.

scholarly journals Microfluidic systems for stem cell-based neural tissue engineering

Lab on a Chip ◽  
2016 ◽  
Vol 16 (14) ◽  
pp. 2551-2571 ◽  
Author(s):  
Mahdi Karimi ◽  
Sajad Bahrami ◽  
Hamed Mirshekari ◽  
Seyed Masoud Moosavi Basri ◽  
Amirala Bakhshian Nik ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Cell Culture ◽  
Stem Cell ◽  
Neural Tissue ◽  
Microfluidic Systems ◽  
Neural Tissue Engineering ◽  
Stem Cell Culture ◽  
Stem Cell Derivation

Overall process of stem cell derivation and isolation, as well as microfluidic stem cell culture and neural tissue engineering.

The study of P19 stem cell behavior on aligned oriented electrospun poly(lactic-co-glycolic acid) nano-fibers for neural tissue engineering

2014 ◽  
Vol 25 (5) ◽  
pp. 562-567 ◽  
Author(s):  
Shiva Irani ◽  
Mojgan Zandi ◽  
Najmeh Salamian ◽  
Seyed Mahdi Saeed ◽  
Morteza Daliri Joupari ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cell ◽  
Glycolic Acid ◽  
Neural Tissue ◽  
Cell Behavior ◽  
Neural Tissue Engineering
Sign in / Sign up

Export Citation Format

Share Document