scholarly journals Advancing fabrication and properties of three-dimensional graphene–alginate scaffolds for application in neural tissue engineering

RSC Advances ◽  
2019 ◽  
Vol 9 (63) ◽  
pp. 36838-36848
Author(s):  
Negar Mansouri ◽  
Said F. Al-Sarawi ◽  
Jagan Mazumdar ◽  
Dusan Losic
Keyword(s):  
Tissue Engineering ◽  
Three Dimensional ◽  
Neural Tissue ◽  
Fabrication Method ◽  
Neural Tissue Engineering ◽  
Composite Scaffolds ◽  
3D Graphene

In this study, a bio-fabrication method has been developed for the preparation of 3D graphene–alginate composite scaffolds with great potential for neural tissue engineering.

Utilizing stem cells for three-dimensional neural tissue engineering

2016 ◽  
Vol 4 (5) ◽  
pp. 768-784 ◽  
Author(s):  
Stephanie Knowlton ◽  
Yongku Cho ◽  
Xue-Jun Li ◽  
Ali Khademhosseini ◽  
Savas Tasoglu
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Three Dimensional ◽  
Neural Tissue ◽  
Disease Models ◽  
Neural Tissue Engineering

Three-dimensional neural tissue engineering has significantly advanced the development of neural disease models and replacement tissues for patients by leveraging the unique capabilities of stem cells.

Three-Dimensional Collagen Gel Networks for Neural Stem Cell-Based Neural Tissue Engineering

2005 ◽  
Vol 227 (1) ◽  
pp. 327-334 ◽  
Author(s):  
Wu Ma ◽  
Silvia Chen ◽  
Wendy Fitzgerald ◽  
Dragan Maric ◽  
Hsingch J. Lin ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cell ◽  
Neural Stem Cell ◽  
Three Dimensional ◽  
Collagen Gel ◽  
Neural Tissue ◽  
Neural Tissue Engineering

scholarly journals Pyrrole Plasma Polymer-Coated Electrospun Scaffolds for Neural Tissue Engineering

Polymers ◽  
2021 ◽  
Vol 13 (22) ◽  
pp. 3876
Author(s):  
Diana María Osorio-Londoño ◽  
José Rafael Godínez-Fernández ◽  
Ma. Cristina Acosta-García ◽  
Juan Morales-Corona ◽  
Roberto Olayo-González ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Colorimetric Assay ◽  
Three Dimensional ◽  
Neural Tissue ◽  
Plasma Polymer ◽  
Neural Cells ◽  
Neural Tissue Engineering ◽  
Microscopy Imaging ◽  
Mtt Colorimetric Assay ◽  
Cell Structures

Promising strategies for neural tissue engineering are based on the use of three-dimensional substrates for cell anchorage and tissue development. In this work, fibrillar scaffolds composed of electrospun randomly- and aligned-oriented fibers coated with plasma synthesized pyrrole polymer, doped and undoped with iodine, were fabricated and characterized. Infrared spectroscopy, thermogravimetric analysis, and X-ray diffraction analysis revealed the functional groups and molecular integration of each scaffold, as well as the effect of plasma polymer synthesis on crystallinity. Scanning microscopy imaging demonstrated the porous fibrillar micrometric structure of the scaffolds, which afforded adhesion, infiltration, and survival for the neural cells. Orientation analysis of electron microscope images confirmed the elongation of neurite-like cell structures elicited by undoped plasma pyrrole polymer-coated aligned scaffolds, without any biochemical stimuli. The MTT colorimetric assay validated the biocompatibility of the fabricated composite materials, and further evidenced plasma pyrrole polymer-coated aligned scaffolds as permissive substrates for the support of neural cells. These results suggest plasma synthesized pyrrole polymer-coated aligned scaffolds are promising materials for tissue engineering applications.

Neural tissue engineering: nanofiber-hydrogel based composite scaffolds

2015 ◽  
Vol 27 (1) ◽  
pp. 42-51 ◽  
Author(s):  
Namdev B. Shelke ◽  
Paul Lee ◽  
Matthew Anderson ◽  
Nikhil Mistry ◽  
Rajaram K. Nagarale ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Neural Tissue ◽  
Neural Tissue Engineering ◽  
Composite Scaffolds

scholarly journals Fabrication of Graphene Oxide Aerogel to Repair Neural Tissue

2021 ◽  
Vol In Press (In Press) ◽  
Author(s):  
Khadijeh Zeinali ◽  
Mohammad Taghi Khorasani ◽  
Alimorad Rashidi ◽  
Morteza Daliri Jouparid
Keyword(s):  
Tissue Engineering ◽  
Graphene Oxide ◽  
Reduced Graphene Oxide ◽  
Three Dimensional ◽  
Neural Tissue ◽  
Nerve Tissue ◽  
Neural Tissue Engineering ◽  
Tissue Scaffold ◽  
Reduced Graphene ◽  
Graphene Oxide Aerogel

: The neural tissue engineering has been designed as a subset of tissue engineering for treating congenital malformations and accident injuries, particularly for individuals requiring tissue grafting. Such transplants, usually performed as autografting, can often not meet the requirements of an effective scaffold used in nerve tissue engineering. A novel neural tissue scaffold was introduced here to solve the problem concerning the reduced graphene oxide. The three-dimensional graphene oxide in the neural canal restricts the formation of fibroglandular tissues and facilities neural stem cell proliferation and growth. In these techniques, graphene oxide aerogel was initially made. Then, the freeze-drying process was used to fix the geometry of reduced graphene oxide hydrogels prepared using graphene oxide dispersion and ethylenediamine and gain aerogels. The X-ray diffraction patterns, FTIR and morphological related to samples were examined, followed by conducting in-vitro micropropagation and 4, 6-diamidino-2-phenylindol (DAPI) staining in fibroblast and P19 cultures. The results from immunofluorescence staining demonstrated the neural differentiation of P19 cells. It can be concluded that most cells attached to and differentiated on the scaffold surface and axons can penetrate randomly through them. Finally, the three-dimensional graphene oxide was proposed as an ideal alternative to be used in neural tissue engineering.

scholarly journals Three-dimensionally printed polycaprolactone/multicomponent bioactive glass scaffolds for potential application in bone tissue engineering

2020 ◽  
Vol 6 (1) ◽  
pp. 57-69
Author(s):  
Amirhosein Fathi ◽  
Farzad Kermani ◽  
Aliasghar Behnamghader ◽  
Sara Banijamali ◽  
Masoud Mozafari ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
3D Printing ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Three Dimensional ◽  
Layer By Layer ◽  
Composite Scaffolds ◽  
3D Printed ◽  
Co Doped

AbstractOver the last years, three-dimensional (3D) printing has been successfully applied to produce suitable substitutes for treating bone defects. In this work, 3D printed composite scaffolds of polycaprolactone (PCL) and strontium (Sr)- and cobalt (Co)-doped multi-component melt-derived bioactive glasses (BGs) were prepared for bone tissue engineering strategies. For this purpose, 30% of as-prepared BG particles (size <38 μm) were incorporated into PCL, and then the obtained composite mix was introduced into a 3D printing machine to fabricate layer-by-layer porous structures with the size of 12 × 12 × 2 mm3.The scaffolds were fully characterized through a series of physico-chemical and biological assays. Adding the BGs to PCL led to an improvement in the compressive strength of the fabricated scaffolds and increased their hydrophilicity. Furthermore, the PCL/BG scaffolds showed apatite-forming ability (i.e., bioactivity behavior) after being immersed in simulated body fluid (SBF). The in vitro cellular examinations revealed the cytocompatibility of the scaffolds and confirmed them as suitable substrates for the adhesion and proliferation of MG-63 osteosarcoma cells. In conclusion, 3D printed composite scaffolds made of PCL and Sr- and Co-doped BGs might be potentially-beneficial bone replacements, and the achieved results motivate further research on these materials.

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