Fluorescent S1 nuclease assay utilizing exponential strand displacement amplification

The Analyst ◽  
2019 ◽  
Vol 144 (10) ◽  
pp. 3364-3368 ◽  
Author(s):  
Chang Yeol Lee ◽  
Hansol Kim ◽  
Hyo Yong Kim ◽  
Ki Soo Park ◽  
Hyun Gyu Park
Keyword(s):  
Nuclease Activity ◽  
Label Free ◽  
Strand Displacement ◽  
Activity Assay ◽  
Strand Displacement Amplification ◽  
S1 Nuclease ◽  
Nuclease Assay

We devise a simple, label-free S1 nuclease activity assay by exploiting target-induced inhibition of exponential strand displacement amplification (eSDA).

TiO2 Nanoparticle-Enhanced Linker Recombinant Strand Displacement Amplification (LRSDA) for Universal Label-Free Visual Bioassays

2019 ◽  
Vol 11 (50) ◽  
pp. 46504-46514 ◽  
Author(s):  
Jingjing Tian ◽  
Huashuo Chu ◽  
Yuan Zhang ◽  
Kai Li ◽  
Hongtao Tian ◽  
...  
Keyword(s):  
Tio2 Nanoparticle ◽  
Label Free ◽  
Strand Displacement ◽  
Strand Displacement Amplification

scholarly journals A label-free protamine-assisted colorimetric sensor for highly sensitive detection of S1 nuclease activity

The Analyst ◽  
2020 ◽  
Vol 145 (7) ◽  
pp. 2774-2778
Author(s):  
Jiahui Wen ◽  
Yongming Liu ◽  
Jingwen Li ◽  
Hao Lin ◽  
Yiran Zheng ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Colorimetric Method ◽  
Nuclease Activity ◽  
Colorimetric Sensor ◽  
Sensitive Detection ◽  
Label Free ◽  
S1 Nuclease ◽  
Highly Sensitive ◽  
Highly Sensitive Detection

A label-free, simple and rapid colorimetric method for the sensitive detection of S1 nuclease activity based on protamine-assisted aggregation of gold nanoparticles.

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