A novel label-free fluorescence strategy for methyltransferase activity assay based on dsDNA-templated copper nanoparticles coupled with an endonuclease-assisted signal transduction system

The Analyst ◽  
2016 ◽  
Vol 141 (4) ◽  
pp. 1383-1389 ◽  
Author(s):  
Q. Q. Lai ◽  
M. D. Liu ◽  
C. C. Gu ◽  
H. G. Nie ◽  
X. J. Xu ◽  
...  
Keyword(s):  
Signal Transduction ◽  
Copper Nanoparticles ◽  
Fluorescence Method ◽  
Label Free ◽  
Activity Assay ◽  
Signal Transduction System ◽  
Methyltransferase Activity ◽  
System P

A novel label-free fluorescence method by coupling dsDNA-templated CuNPs with endonuclease-assisted signal transduction has been developed for methyltransferase activity assay.

A label-free aptasensor for highly sensitive ATP detection by using exonuclease I and oligonucleotide-templated fluorescent copper nanoparticles

2017 ◽  
Vol 9 (18) ◽  
pp. 2710-2714 ◽  
Author(s):  
Qiyong Cai ◽  
Jia Ge ◽  
Huihui Xu ◽  
Lin Zhang ◽  
Yalei Hu ◽  
...  
Keyword(s):  
Copper Nanoparticles ◽  
Fluorescence Method ◽  
Label Free ◽  
Exonuclease I ◽  
Highly Sensitive ◽  
Atp Detection

A sensitive fluorescence method for ATP detection was developed based on Exo I and a multifunctional oligonucleotide, which holds the ability of synthesizing fluorescent copper nanoparticles and recognizing ATP.

Label-free and near-zero-background-noise photoelectrochemical assay of methyltransferase activity based on a Bi2S3/Ti3C2 Schottky junction

2020 ◽  
Vol 56 (43) ◽  
pp. 5799-5802 ◽  
Author(s):  
Yamin Fu ◽  
Feng Ding ◽  
Jinhua Chen ◽  
Mengyue Liu ◽  
Xiaohua Zhang ◽  
...  
Keyword(s):  
Background Noise ◽  
Schottky Junction ◽  
Label Free ◽  
Activity Assay ◽  
Methyltransferase Activity ◽  
Sensing Platform ◽  
Photoelectrochemical Assay

Based on Bi2S3/Ti3C2 nanosheets, a label-free photoelectrochemical sensing platform with near-zero background noise was developed for M.SssI methyltransferase activity assay.

scholarly journals Redox switching of an artificial transmembrane signal transduction system

2021 ◽  
Vol 57 (17) ◽  
pp. 2196-2198
Author(s):  
Lucia Trevisan ◽  
Istvan Kocsis ◽  
Christopher A. Hunter
Keyword(s):  
Signal Transduction ◽  
Ascorbic Acid ◽  
Membrane Translocation ◽  
Signal Transduction System ◽  
System P ◽  
Redox Switching

An external redox signal delivered by ascorbic acid was used to trigger membrane translocation in a synthetic transduction system.

Ultrasensitive and turn-on homogeneous Hg2+ sensing based on target-triggered isothermal cycling reaction and dsDNA-templated copper nanoparticles

2021 ◽  
Author(s):  
Qingli Chai ◽  
Yuqi Wan ◽  
Yanyun Zou ◽  
Ting Zhu ◽  
Ningxing Li ◽  
...  
Keyword(s):  
Copper Nanoparticles ◽  
Label Free ◽  
Turn On ◽  
Signal Output

In this work, an ultrasensitive and turn-on sensor for homogeneous Hg2+ detection has been constructed based on target-triggered isothermal cycling reaction and rapid label-free signal output with dsDNA-templated copper nanoparticles...

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