Label-free and near-zero-background-noise photoelectrochemical assay of methyltransferase activity based on a Bi2S3/Ti3C2 Schottky junction

2020 ◽  
Vol 56 (43) ◽  
pp. 5799-5802 ◽  
Author(s):  
Yamin Fu ◽  
Feng Ding ◽  
Jinhua Chen ◽  
Mengyue Liu ◽  
Xiaohua Zhang ◽  
...  
Keyword(s):  
Background Noise ◽  
Schottky Junction ◽  
Label Free ◽  
Activity Assay ◽  
Methyltransferase Activity ◽  
Sensing Platform ◽  
Photoelectrochemical Assay

Based on Bi2S3/Ti3C2 nanosheets, a label-free photoelectrochemical sensing platform with near-zero background noise was developed for M.SssI methyltransferase activity assay.

Immuno-DNA binding directed template-free DNA extension and enzyme catalysis for sensitive electrochemical DNA methyltransferase activity assay and inhibitor screening

The Analyst ◽  
2020 ◽  
Vol 145 (8) ◽  
pp. 3064-3072 ◽  
Author(s):  
Ying Zhang ◽  
Lijie Hao ◽  
Zhen Zhao ◽  
Xiaoyan Yang ◽  
Li Wang ◽  
...  
Keyword(s):  
Dna Binding ◽  
Enzyme Catalysis ◽  
Dna Methyltransferase ◽  
Dna Sensing ◽  
Activity Assay ◽  
Methyltransferase Activity ◽  
Inhibitor Screening ◽  
Free Dna ◽  
Sensing Platform ◽  
Template Free

A new electrochemical immuno-DNA sensing platform for DNA methyltransferase activity assay and inhibitor screening.

A novel label-free fluorescence strategy for methyltransferase activity assay based on dsDNA-templated copper nanoparticles coupled with an endonuclease-assisted signal transduction system

The Analyst ◽  
2016 ◽  
Vol 141 (4) ◽  
pp. 1383-1389 ◽  
Author(s):  
Q. Q. Lai ◽  
M. D. Liu ◽  
C. C. Gu ◽  
H. G. Nie ◽  
X. J. Xu ◽  
...  
Keyword(s):  
Signal Transduction ◽  
Copper Nanoparticles ◽  
Fluorescence Method ◽  
Label Free ◽  
Activity Assay ◽  
Signal Transduction System ◽  
Methyltransferase Activity ◽  
System P

A novel label-free fluorescence method by coupling dsDNA-templated CuNPs with endonuclease-assisted signal transduction has been developed for methyltransferase activity assay.

A label-free and enzyme-free signal amplification strategy for a sensitive RNase H activity assay

Nanoscale ◽  
2017 ◽  
Vol 9 (42) ◽  
pp. 16149-16153 ◽  
Author(s):  
Chang Yeol Lee ◽  
Hyowon Jang ◽  
Ki Soo Park ◽  
Hyun Gyu Park
Keyword(s):  
Signal Amplification ◽  
Rnase H ◽  
Label Free ◽  
Activity Assay ◽  
G Quadruplex ◽  
Catalytic Hairpin Assembly ◽  
Amplification Strategy

A target-triggered catalytic hairpin assembly with a G-quadruplex specific fluorescent binder, NMM, is employed to develop a novel and sensitive RNase H activity assay.

scholarly journals An Aptamer-Based Capacitive Sensing Platform for Specific Detection of Lung Carcinoma Cells in the Microfluidic Chip

Biosensors ◽  
2018 ◽  
Vol 8 (4) ◽  
pp. 98 ◽  
Author(s):  
Ngoc-Viet Nguyen ◽  
Chun-Hao Yang ◽  
Chung-Jung Liu ◽  
Chao-Hung Kuo ◽  
Deng-Chyang Wu ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Lung Carcinoma ◽  
Early Stage ◽  
A549 Cells ◽  
Microfluidic Channel ◽  
Electrical Impedance Spectroscopy ◽  
Label Free ◽  
Early Stage Lung Cancer ◽  
Sensing Platform ◽  
Label Free Detection

Improvement of methods for reliable and early diagnosis of the cellular diseases is necessary. A biological selectivity probe, such as an aptamer, is one of the candidate recognition layers that can be used to detect important biomolecules. Lung cancer is currently a typical cause of cancer-related deaths. In this work, an electrical sensing platform is built based on amine-terminated aptamer modified-gold electrodes for the specific, label-free detection of a human lung carcinoma cell line (A549). The microdevice, that includes a coplanar electrodes configuration and a simple microfluidic channel on a glass substrate, is fabricated using standard photolithography and cast molding techniques. A procedure of self-assembly onto the gold surface is proposed. Optical microscope observations and electrical impedance spectroscopy measurements confirm that the fabricated microchip can specifically and effectively identify A549 cells. In the experiments, the capacitance element that is dominant in the change of the impedance is calculated at the appropriate frequency for evaluation of the sensitivity of the biosensor. Therefore, a simple, inexpensive, biocompatible, and selective biosensor that has the potential to detect early-stage lung cancer would be developed.

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