A label-free aptasensor for highly sensitive ATP detection by using exonuclease I and oligonucleotide-templated fluorescent copper nanoparticles

2017 ◽  
Vol 9 (18) ◽  
pp. 2710-2714 ◽  
Author(s):  
Qiyong Cai ◽  
Jia Ge ◽  
Huihui Xu ◽  
Lin Zhang ◽  
Yalei Hu ◽  
...  
Keyword(s):  
Copper Nanoparticles ◽  
Fluorescence Method ◽  
Label Free ◽  
Exonuclease I ◽  
Highly Sensitive ◽  
Atp Detection

A sensitive fluorescence method for ATP detection was developed based on Exo I and a multifunctional oligonucleotide, which holds the ability of synthesizing fluorescent copper nanoparticles and recognizing ATP.

Label-free biosensor based on dsDNA-templated copper nanoparticles for highly sensitive and selective detection of NAD+

RSC Advances ◽  
2016 ◽  
Vol 6 (94) ◽  
pp. 91077-91082 ◽  
Author(s):  
Jia Ge ◽  
Zhen-Zhen Dong ◽  
Lin Zhang ◽  
Qi-Yong Cai ◽  
Dong-Mei Bai ◽  
...  
Keyword(s):  
Copper Nanoparticles ◽  
Selective Detection ◽  
Label Free ◽  
Ligation Reaction ◽  
Dna Ligation ◽  
Highly Sensitive

A novel label-free biosensor for high sensing of NAD+ based on dsDNA-templated CuNPs and DNA ligation reaction.

scholarly journals A Label-Free Fluorescent Sensor Based on the Formation of Poly(thymine)-Templated Copper Nanoparticles for the Sensitive and Selective Detection of MicroRNA from Cancer Cells

Chemosensors ◽  
2020 ◽  
Vol 8 (3) ◽  
pp. 52
Author(s):  
Qian Ma ◽  
Zhiqiang Gao ◽  
Hiranya Dayal ◽  
Sam Fong Yau Li
Keyword(s):  
Cancer Cells ◽  
Copper Nanoparticles ◽  
Fluorescent Sensor ◽  
Rolling Circle Amplification ◽  
High Specificity ◽  
Excitation Wavelength ◽  
Selective Detection ◽  
Label Free ◽  
Rolling Circle ◽  
Highly Sensitive

In this work, a simple and label-free fluorescence “off” to “on” platform was designed for the sensitive and selective detection of microRNA (miRNA) in cancer cells. This method utilized a padlock DNA-based rolling circle amplification (P-RCA) to synthesize fluorescent poly(thymine) (PolyT) which acted as a template for the synthesis of copper nanoparticles (CuNPs) within 10 minutes under mild conditions. While the repeated PolyT sequence was used as the template for CuNP synthesis, other non-PolyT parts (single strand-DNAs without the capacity to act as the template for CuNP formation) served as “smart glues” or rigid linkers to build complex nanostructures. Under the excitation wavelength of 340 nm, the synthesized CuNPs emitted strong red fluorescence effectively at 620 nm. To demonstrate the use of this method as a universal biosensor platform, lethal-7a (let-7a) miRNA was chosen as the standard target. This sensor could achieve highly sensitive and selective detection of miRNA in the presence of other homologous analogues for the combination of P-RCA with the fluorescent copper nanoparticle. Overall, this novel label-free method holds great potential in the sensitive detection of miRNA with high specificity in real samples.

Fluorescence assay based on the thioflavin T-induced conformation switch of G-quadruplexes for TET1 detection

The Analyst ◽  
2021 ◽  
Vol 146 (7) ◽  
pp. 2126-2130
Author(s):  
Xue Chen ◽  
Ying Cheng ◽  
Yafen Wang ◽  
Jing Tang ◽  
Fang Wang ◽  
...  
Keyword(s):  
Fluorescence Assay ◽  
Fluorescence Method ◽  
Thioflavin T ◽  
Sensitive Detection ◽  
Label Free ◽  
Specific Design ◽  
Highly Sensitive ◽  
Highly Sensitive Detection

A simple and label-free fluorescence method is developed for the highly sensitive detection of TET1 based on ThT/G-quadruplexes in combination with the specific design of oligonucleotides.

Sensitive and label-free T4 polynucleotide kinase/phosphatase detection based on poly(thymine)-templated copper nanoparticles coupled with nicking enzyme-assisted signal amplification

2016 ◽  
Vol 8 (13) ◽  
pp. 2831-2836 ◽  
Author(s):  
Jia Ge ◽  
Lin Zhang ◽  
Zhen-Zhen Dong ◽  
Qi-Yong Cai ◽  
Zhao-Hui Li
Keyword(s):  
Copper Nanoparticles ◽  
Signal Amplification ◽  
Label Free ◽  
Free T4 ◽  
Highly Sensitive ◽  
Polynucleotide Kinase ◽  
Nicking Enzyme ◽  
T4 Polynucleotide Kinase

A novel label-free biosensor for highly sensitive sensing of the activity as well as inhibition of T4 PNKP based on poly T-templated fluorescent CuNPs and nicking enzyme-assisted signal amplification.

An exonuclease I-based label-free fluorometric aptasensor for adenosine triphosphate (ATP) detection with a wide concentration range

2015 ◽  
Vol 63 ◽  
pp. 311-316 ◽  
Author(s):  
Yanli Wei ◽  
Yanxia Chen ◽  
Huanhuan Li ◽  
Shaomin Shuang ◽  
Chuan Dong ◽  
...  
Keyword(s):  
Adenosine Triphosphate ◽  
Wide Concentration Range ◽  
Label Free ◽  
Exonuclease I ◽  
Atp Detection

A label-free G-quadruplex DNA-based fluorescence method for highly sensitive, direct detection of cisplatin

2014 ◽  
Vol 202 ◽  
pp. 714-720 ◽  
Author(s):  
Hualin Yang ◽  
Hanli Cui ◽  
Longgang Wang ◽  
Li Yan ◽  
Yong Qian ◽  
...  
Keyword(s):  
Direct Detection ◽  
Fluorescence Method ◽  
Label Free ◽  
Quadruplex Dna ◽  
Highly Sensitive ◽  
G Quadruplex

scholarly journals A Label-Free Fluorescent AND Logic Gate Aptasensor for Sensitive ATP Detection

Sensors ◽  
2018 ◽  
Vol 18 (10) ◽  
pp. 3281 ◽  
Author(s):  
Jingjing Zhang ◽  
Chunzheng Yang ◽  
Chaoqun Niu ◽  
Chen Liu ◽  
Xuepin Cai ◽  
...  
Keyword(s):  
Deoxyribonucleic Acid ◽  
Limit Of Detection ◽  
Low Cost ◽  
Logic Gate ◽  
Label Free ◽  
Detection Range ◽  
Sensitivity Range ◽  
Highly Sensitive ◽  
Input Signals ◽  
Atp Detection

In this study, a label-free fluorescent, enzyme-free, simple, highly sensitive AND logic gate aptasensor was developed for the detection of adenosine triphosphate (ATP). Double-stranded deoxyribonucleic acid (DNA) with cohesive ends was attached to graphene oxide (GO) to form an aptasensor probe. ATP and single-stranded DNA were used as input signals. Fluorescence intensity of PicoGreen dye was used as an output signal. The biosensor-related performances, including the logic gate construction, reaction time, linearity, sensitivity, and specificity, were investigated and the results showed that an AND logic gate was successfully constructed. The ATP detection range was found to be 20 to 400 nM (R2 = 0.9943) with limit of detection (LOD) of 142.6 pM, and the sensitivity range was 1.846 × 106 to 2.988 × 106 M−1. This method for the detection of ATP has the characteristics of being simple, low cost, and highly sensitive.

A novel label-free fluorescence strategy for methyltransferase activity assay based on dsDNA-templated copper nanoparticles coupled with an endonuclease-assisted signal transduction system

The Analyst ◽  
2016 ◽  
Vol 141 (4) ◽  
pp. 1383-1389 ◽  
Author(s):  
Q. Q. Lai ◽  
M. D. Liu ◽  
C. C. Gu ◽  
H. G. Nie ◽  
X. J. Xu ◽  
...  
Keyword(s):  
Signal Transduction ◽  
Copper Nanoparticles ◽  
Fluorescence Method ◽  
Label Free ◽  
Activity Assay ◽  
Signal Transduction System ◽  
Methyltransferase Activity ◽  
System P

A novel label-free fluorescence method by coupling dsDNA-templated CuNPs with endonuclease-assisted signal transduction has been developed for methyltransferase activity assay.

scholarly journals Optical Monitoring of the Production Quality of Si-Nanoribbon Chips Intended for the Detection of ASD-Associated Oligonucleotides

Micromachines ◽  
2021 ◽  
Vol 12 (2) ◽  
pp. 147
Author(s):  
Kristina A. Malsagova ◽  
Tatyana O. Pleshakova ◽  
Vladimir P. Popov ◽  
Igor N. Kupriyanov ◽  
Rafael A. Galiullin ◽  
...  
Keyword(s):  
Production Quality ◽  
Autism Spectrum ◽  
Covalent Immobilization ◽  
Biological Macromolecules ◽  
Label Free ◽  
Raman Scattering Spectroscopy ◽  
Dna Oligonucleotides ◽  
Highly Sensitive ◽  
Highly Sensitive Detection

Gas-phase etching and optical lithography were employed for the fabrication of a silicon nanoribbon chip (Si-NR chip). The quality of the so-fabricated silicon nanoribbons (Si-NRs) was monitored by optical Raman scattering spectroscopy. It was demonstrated that the structures of the Si-NRs were virtually defect-free, meaning they could be used for highly sensitive detection of biological macromolecules. The Si-NR chips were then used for the highly sensitive nanoelectronics detection of DNA oligonucleotides (oDNAs), which represent synthetic analogs of 106a-5p microRNA (miR-106a-5p), associated with the development of autism spectrum disorders in children. The specificity of the analysis was attained by the sensitization of the Si-NR chip sur-face by covalent immobilization of oDNA probes, whose nucleotide sequence was complementary to the known sequence of miR-106a-5p. The use of the Si-NR chip was demonstrated to al-low for the rapid label-free real-time detection of oDNA at ultra-low (~10−17 M) concentrations.

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