Nicking endonuclease-assisted signal amplification of a split molecular aptamer beacon for biomolecule detection using graphene oxide as a sensing platform

The Analyst ◽  
2015 ◽  
Vol 140 (23) ◽  
pp. 7918-7925 ◽  
Author(s):  
Xiang Li ◽  
Xuelian Ding ◽  
Jing Fan
Keyword(s):  
Graphene Oxide ◽  
Signal Amplification ◽  
Biomolecule Detection ◽  
Target Recycling ◽  
Nicking Endonuclease ◽  
Sensing Platform ◽  
Detection Of Biomolecules ◽  
Aptamer Beacon

A highly selective and sensitive amplified fluorescence aptasensor for detection of biomolecules was developed based on enzyme-assisted target-recycling signal amplification and graphene oxide.

Carbon nanoparticle-protected aptamers for highly sensitive and selective detection of biomolecules based on nuclease-assisted target recycling signal amplification

2014 ◽  
Vol 50 (57) ◽  
pp. 7646-7648 ◽  
Author(s):  
Xiaoyan Lin ◽  
Liang Cui ◽  
Yishun Huang ◽  
Ya Lin ◽  
Yi Xie ◽  
...  
Keyword(s):  
Signal Amplification ◽  
Carbon Nanoparticles ◽  
Sensitive Detection ◽  
Selective Detection ◽  
Carbon Nanoparticle ◽  
Target Recycling ◽  
Amplification Method ◽  
Highly Sensitive ◽  
Detection Of Biomolecules ◽  
Highly Sensitive Detection

A nuclease-assisted target recycling signal amplification method based on carbon nanoparticles for highly sensitive detection of biomolecules was developed.

A potentiometric aptasensor for carcinoembryonic antigen (CEA) on graphene oxide nanosheets using catalytic recycling of DNase I with signal amplification

2018 ◽  
Vol 10 (45) ◽  
pp. 5364-5371 ◽  
Author(s):  
Zhilin Hong ◽  
Genwang Chen ◽  
Shaoyang Yu ◽  
Rongfu Huang ◽  
Chunmei Fan
Keyword(s):  
Graphene Oxide ◽  
Carcinoembryonic Antigen ◽  
Modified Electrode ◽  
Signal Amplification ◽  
Dnase I ◽  
Label Free ◽  
Graphene Oxide Nanosheets ◽  
Target Recycling

A label-free potentiometric aptasensing platform was designed for detection of carcinoembryonic antigen on a graphene oxide-modified electrode coupling with target recycling-assisted signal amplification.

A TiS2 nanosheet enhanced fluorescence polarization biosensor for ultra-sensitive detection of biomolecules

Nanoscale ◽  
2016 ◽  
Vol 8 (18) ◽  
pp. 9852-9860 ◽  
Author(s):  
Xiang Li ◽  
Xuelian Ding ◽  
Yongfang Li ◽  
Linsong Wang ◽  
Jing Fan
Keyword(s):  
Fluorescence Polarization ◽  
Signal Amplification ◽  
Sensitive Detection ◽  
Enhanced Fluorescence ◽  
Target Recycling ◽  
Detection Of Biomolecules

A highly selective and sensitive amplified fluorescence polarization (FP) biosensor for detection of biomolecules was developed based on enzyme-assisted target-recycling signal amplification and TiS2 nanosheet.

scholarly journals Amplified Fluorescent Aptasensor for Ochratoxin A Assay Based on Graphene Oxide and RecJf Exonuclease

Toxins ◽  
2020 ◽  
Vol 12 (11) ◽  
pp. 670
Author(s):  
Han Zhao ◽  
Dehui Xiong ◽  
Ying Yan ◽  
Changbei Ma
Keyword(s):  
Graphene Oxide ◽  
Ochratoxin A ◽  
Signal Amplification ◽  
Red Wine ◽  
Limit Of Detection ◽  
Experimental Conditions ◽  
Fluorescent Sensing ◽  
Sensing System ◽  
Sensing Platform ◽  
Fluorescent Response

In this study, we developed an aptamer-based fluorescent sensing platform for the detection of ochratoxin A (OTA) based on RecJf exonuclease-assisted signal amplification and interaction between graphene oxide (GO) and the OTA aptamer (OTA-apt). After optimizing the experimental conditions, the present aptamer-based sensing system can exhibit excellent fluorescent response in the OTA assay, with a limit of detection of 0.07 ng/mL. In addition to signal amplification, this strategy is also highly specific for other interfering toxins. Furthermore, this aptasensor can be reliably used for assessing red wine samples spiked with different OTA concentrations (2.4, 6 and 20 ng/mL). The proposed assay plays an important role in the field of food safety and can be transformed for detecting other toxins by replacing the sequence that recognizes the aptamer.

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