Ultrasensitive electrochemical sensing platform for microRNA based on tungsten oxide-graphene composites coupling with catalyzed hairpin assembly target recycling and enzyme signal amplification

2016 ◽  
Vol 86 ◽  
pp. 337-345 ◽  
Author(s):  
Hong-Lei Shuai ◽  
Ke-Jing Huang ◽  
Ling-Li Xing ◽  
Ying-Xu Chen
Keyword(s):  
Tungsten Oxide ◽  
Signal Amplification ◽  
Electrochemical Sensing ◽  
Target Recycling ◽  
Sensing Platform ◽  
Catalyzed Hairpin Assembly

scholarly journals An ultrasensitive electrochemical sensing platform for the detection of cTnI based on aptamer recognition and signal amplification assisted by TdT

RSC Advances ◽  
2020 ◽  
Vol 10 (60) ◽  
pp. 36396-36403
Author(s):  
Mingjian Lang ◽  
Dan Luo ◽  
Guangyi Yang ◽  
Quanxi Mei ◽  
Guangjun Feng ◽  
...  
Keyword(s):  
Myocardial Infarction ◽  
Acute Myocardial Infarction ◽  
Cardiovascular Diseases ◽  
Cardiac Troponin ◽  
Signal Amplification ◽  
Troponin I ◽  
Cardiac Troponin I ◽  
Electrochemical Sensing ◽  
Sensing Platform

We have developed an ultrasensitive and highly specific electrochemical sensing platform for the detection of cardiac troponin I (cTnI), a recognized biomarker for the diagnosis of acute myocardial infarction (AMI) and related cardiovascular diseases (CVDs).

Nicking endonuclease-assisted signal amplification of a split molecular aptamer beacon for biomolecule detection using graphene oxide as a sensing platform

The Analyst ◽  
2015 ◽  
Vol 140 (23) ◽  
pp. 7918-7925 ◽  
Author(s):  
Xiang Li ◽  
Xuelian Ding ◽  
Jing Fan
Keyword(s):  
Graphene Oxide ◽  
Signal Amplification ◽  
Biomolecule Detection ◽  
Target Recycling ◽  
Nicking Endonuclease ◽  
Sensing Platform ◽  
Detection Of Biomolecules ◽  
Aptamer Beacon

A highly selective and sensitive amplified fluorescence aptasensor for detection of biomolecules was developed based on enzyme-assisted target-recycling signal amplification and graphene oxide.

scholarly journals Paper-Based Competitive Immunochromatography Coupled with an Enzyme-Modified Electrode to Enable the Wireless Monitoring and Electrochemical Sensing of Cotinine in Urine

Sensors ◽  
2021 ◽  
Vol 21 (5) ◽  
pp. 1659
Author(s):  
Nutcha Larpant ◽  
Pramod K. Kalambate ◽  
Tautgirdas Ruzgas ◽  
Wanida Laiwattanapaisal
Keyword(s):  
Glucose Oxidase ◽  
Modified Electrode ◽  
Near Field ◽  
Electrochemical Measurement ◽  
Electrochemical Sensing ◽  
Urine Samples ◽  
Lateral Flow Strip ◽  
Wireless Monitoring ◽  
Sensing Platform ◽  
Combined Strategy

This paper proposes a combined strategy of using paper-based competitive immunochromatography and a near field communication (NFC) tag for wireless cotinine determination. The glucose oxidase labeled cotinine antibody specifically binds free cotinine in a sample, whereas the unoccupied antibody attached to BSA-cotinine at the test line on a lateral flow strip. The glucose oxidase on the strip and an assistant pad in the presence of glucose generated H2O2 and imposed the Ag oxidation on the modified electrode. This enabled monitoring of immunoreaction by either electrochemical measurement or wireless detection. Wireless sensing was realized for cotinine in the range of 100–1000 ng/mL (R2 = 0.96) in PBS medium. Undiluted urine samples from non-smokers exhibited an Ag-oxidation rate three times higher than the smoker’s urine samples. For 1:8 diluted urine samples (smokers), the proposed paper-based competitive immunochromatography coupled with an enzyme-modified electrode differentiated positive and negative samples and exhibited cotinine discrimination at levels higher than 12 ng/mL. This novel sensing platform can potentially be combined with a smartphone as a reader unit.

scholarly journals MiRNA Detection Using a Rolling Circle Amplification and RNA-Cutting Allosteric Deoxyribozyme Dual Signal Amplification Strategy

Biosensors ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 222
Author(s):  
Chenxin Fang ◽  
Ping Ouyang ◽  
Yuxing Yang ◽  
Yang Qing ◽  
Jialun Han ◽  
...  
Keyword(s):  
Signal Amplification ◽  
Rolling Circle Amplification ◽  
Padlock Probe ◽  
Rolling Circle ◽  
Sensing Platform ◽  
Mirna Detection ◽  
Substrate Cleavage ◽  
Amplification Strategy ◽  
Circular Template ◽  
Dual Signal Amplification

A microRNA (miRNA) detection platform composed of a rolling circle amplification (RCA) system and an allosteric deoxyribozyme system is proposed, which can detect miRNA-21 rapidly and efficiently. Padlock probe hybridization with the target miRNA is achieved through complementary base pairing and the padlock probe forms a closed circular template under the action of ligase; this circular template results in RCA. In the presence of DNA polymerase, RCA proceeds and a long chain with numerous repeating units is formed. In the presence of single-stranded DNA (H1 and H2), multi-component nucleic acid enzymes (MNAzymes) are formed that have the ability to cleave substrates. Finally, substrates containing fluorescent and quenching groups and magnesium ions are added to the system to activate the MNAzyme and the substrate cleavage reaction, thus achieving fluorescence intensity amplification. The RCA–MNAzyme system has dual signal amplification and presents a sensing platform that demonstrates broad prospects in the analysis and detection of nucleic acids.

Copper sulfide nano-globules reinforced electrodes for high-performance electrochemical determination of toxic pollutant hydroquinone

2021 ◽  
Vol 45 (6) ◽  
pp. 3215-3223
Author(s):  
Selvarasu Maheshwaran ◽  
Ramachandran Balaji ◽  
Shen-Ming Chen ◽  
Ray Biswadeep ◽  
Vengudusamy Renganathan ◽  
...  
Keyword(s):  
Copper Sulfide ◽  
High Performance ◽  
Electrochemical Determination ◽  
Electrochemical Sensing ◽  
Sensing Platform ◽  
Toxic Pollutant

A high-performance electrochemical sensing platform based on CuS nano-globules is efficiently developed.

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