Integrated platform with a combination of online digestion and 18O labeling for proteome quantification via an immobilized trypsin microreactor

The Analyst ◽  
2015 ◽  
Vol 140 (15) ◽  
pp. 5227-5234 ◽  
Author(s):  
Shen Zhang ◽  
Huiming Yuan ◽  
Baofeng Zhao ◽  
Yuan Zhou ◽  
Hao Jiang ◽  
...  

A novel integrated platform for quantitative proteome analysis was established by the combination of IMER, for digestion and in situ18O labeling, and nanoRPLC-ESI-MS/MS.

Micromachines ◽  
2021 ◽  
Vol 12 (8) ◽  
pp. 929
Author(s):  
Xudong Yang ◽  
Zexiao Li ◽  
Linlin Zhu ◽  
Yuchu Dong ◽  
Lei Liu ◽  
...  

Taper-cutting experiments are important means of exploring the nano-cutting mechanisms of hard and brittle materials. Under current cutting conditions, the brittle-ductile transition depth (BDTD) of a material can be obtained through a taper-cutting experiment. However, taper-cutting experiments mostly rely on ultra-precision machining tools, which have a low efficiency and high cost, and it is thus difficult to realize in situ measurements. For taper-cut surfaces, three-dimensional microscopy and two-dimensional image calculation methods are generally used to obtain the BDTDs of materials, which have a great degree of subjectivity, leading to low accuracy. In this paper, an integrated system-processing platform is designed and established in order to realize the processing, measurement, and evaluation of taper-cutting experiments on hard and brittle materials. A spectral confocal sensor is introduced to assist in the assembly and adjustment of the workpiece. This system can directly perform taper-cutting experiments rather than using ultra-precision machining tools, and a small white light interference sensor is integrated for in situ measurement of the three-dimensional topography of the cutting surface. A method for the calculation of BDTD is proposed in order to accurately obtain the BDTDs of materials based on three-dimensional data that are supplemented by two-dimensional images. The results show that the cutting effects of the integrated platform on taper cutting have a strong agreement with the effects of ultra-precision machining tools, thus proving the stability and reliability of the integrated platform. The two-dimensional image measurement results show that the proposed measurement method is accurate and feasible. Finally, microstructure arrays were fabricated on the integrated platform as a typical case of a high-precision application.


2015 ◽  
Vol 44 (27) ◽  
pp. 12086-12090 ◽  
Author(s):  
Hong-Jie Chen ◽  
Mei-Chun Tseng ◽  
I-Jui Hsu ◽  
Wei-Ting Chen ◽  
Chien-Chung Han ◽  
...  

Complexes [Cu(i)(2,4-dimethylphenoxy)2]− (A) and [Cu(ii)(2,4-dimethylphenoxy)2(p-tolyl)]− (B) were observed by in situ ESI-MS analysis of the ligand free copper(i)-catalyzed C–O coupling reaction using Cs2CO3 under the catalytic reaction conditions.


Talanta ◽  
2020 ◽  
Vol 211 ◽  
pp. 120751 ◽  
Author(s):  
Yan Zheng ◽  
Zhiqiang Liu ◽  
Junpeng Xing ◽  
Zhong Zheng ◽  
Zifeng Pi ◽  
...  

2014 ◽  
Vol 2014 ◽  
pp. 1-12 ◽  
Author(s):  
Stefan Kalkhof ◽  
Yvonne Förster ◽  
Johannes Schmidt ◽  
Matthias C. Schulz ◽  
Sven Baumann ◽  
...  

Wound healing of soft tissue and bone defects is a complex process in which cellular differentiation and adaption are regulated by internal and external factors, among them are many different proteins. In contrast to insights into the significance of various single proteins based on model systems, the knowledge about the processes at the actual site of wound healing is still limited. This is caused by a general lack of methods that allow sampling of extracellular factors, metabolites, and proteinsin situ. Sampling of wound fluids in combination with proteomics and metabolomics is one of the promising approaches to gain comprehensive and time resolved data on effector molecules. Here, we describe an approach to sample metabolites by microdialysis and to extract proteins simultaneously by adsorption. With this approach it is possible (i) to collect, enrich, and purify proteins for a comprehensive proteome analysis; (ii) to detect more than 600 proteins in different defects including more than 100 secreted proteins, of which many proteins have previously been demonstrated to have diagnostic or predictive power for the wound healing state; and (iii) to combine continuous sampling of cytokines and metabolites and discontinuous sampling of larger proteins to gain complementary information of the same defect.


2010 ◽  
Vol 695 (12-13) ◽  
pp. 1708-1714 ◽  
Author(s):  
André L. Bogado ◽  
Marília I.F. Barbosa ◽  
Gezimar D. de Souza ◽  
Marília A. Trappi ◽  
Edson R. Filho ◽  
...  
Keyword(s):  

2009 ◽  
Vol 81 (21) ◽  
pp. 8708-8714 ◽  
Author(s):  
Huiming Yuan ◽  
Lihua Zhang ◽  
Chunyan Hou ◽  
Guijie Zhu ◽  
Dingyin Tao ◽  
...  

2005 ◽  
Vol 4 (2) ◽  
pp. 238-249 ◽  
Author(s):  
Mathias Dreger ◽  
Joanna Mika ◽  
Annette Bieller ◽  
Ricarda Jahnel ◽  
Clemens Gillen ◽  
...  

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