Carboxy-terminated immuno-SERS tags overcome non-specific aggregation for the robust detection and localization of organic media in artworks

The Analyst ◽  
2015 ◽  
Vol 140 (17) ◽  
pp. 5971-5980 ◽  
Author(s):  
E. A. Perets ◽  
A. S. D. S. Indrasekara ◽  
A. Kurmis ◽  
N. Atlasevich ◽  
L. Fabris ◽  
...  
Keyword(s):  
Surface Coating ◽  
Organic Media ◽  
Binding Media ◽  
Robust Detection ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Enhanced Raman Scattering ◽  
Detection And Localization ◽  
Proteinaceous Binding Media ◽  
Sers Tags

Surface-enhanced Raman scattering (SERS) nano-tags with a carboxy-terminated PEG surface coating overcome non-specific aggregation when applied for the immunological detection and localization of proteinaceous binding media in art samples.

scholarly journals Correlation of surface-enhanced Raman scattering (SERS) with the surface density of gold nanoparticles: evaluation of the critical number of SERS tags for a detectable signal

2019 ◽  
Vol 10 ◽  
pp. 1016-1023 ◽  
Author(s):  
Vincenzo Amendola
Keyword(s):  
Gold Nanoparticles ◽  
Raman Scattering ◽  
Surface Density ◽  
Surface Enhanced Raman Scattering ◽  
Operating Conditions ◽  
Acquisition Time ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Enhanced Raman Scattering ◽  
Sers Tags

The use of plasmonic nanotags based on the surface-enhanced Raman scattering (SERS) effect is highly promising for several applications in analytical chemistry, biotechnological assays and nanomedicine. To this end, a crucial parameter is the minimum number of SERS tags that allows for the collection of intense Raman signals under real operating conditions. Here, SERS Au nanotags (AuNTs) based on clustered gold nanoparticles are deposited on a substrate and analyzed in the same region using Raman spectroscopy and transmission electron microscopy. In this way, the Raman spectra and the surface density of the SERS tags are correlated directly, showing that 1 tag/µm2 is enough to generate an intense signal above the noise level at 633 nm with an excitation power of only 0.65 mW and an acquisition time of just 1 s with a 50× objective. The AuNT density can be even lower than 1 tag/µm2 when the acquisition time is extended to 10 s, but must be increased to 3 tags/µm2 when a 20× objective is employed under the same excitation conditions. In addition, in order to observe a linear response, it was found that 10 SERS AuNTs inside the probed area are required. These findings indicate that a better signal-to-noise ratio requires high-magnification optics, while linearity versus tag number can be improved by using low-magnification optics or a high tag density. In general the suitability of plasmonic SERS labels for ultrasensitive analytical and biomedical applications is evident.

scholarly journals Bioanalytical applications of surface-enhanced Raman spectroscopy: de novo molecular identification

2017 ◽  
Vol 36 (4) ◽  
Author(s):  
Anh H. Nguyen ◽  
Emily A. Peters ◽  
Zachary D. Schultz
Keyword(s):  
De Novo ◽  
Surface Enhanced Raman Spectroscopy ◽  
Clinical Diagnostics ◽  
Active Materials ◽  
Intrinsic Signal ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Enhanced Raman Scattering ◽  
Bioanalytical Applications ◽  
Sers Tags

AbstractSurface-enhanced Raman scattering (SERS) has become a powerful technique for trace analysis of biomolecules. The use of SERS-tags has evolved into clinical diagnostics; the enhancement of the intrinsic signal of biomolecules on SERS active materials shows tremendous promise for the analysis of biomolecules and potential biomedical assays. The detection of the

scholarly journals Silica-coated dimers of silver nanospheres as surface-enhanced Raman scattering tags for imaging cancer cells

2013 ◽  
Vol 3 (3) ◽  
pp. 20120092 ◽  
Author(s):  
Xiaohu Xia ◽  
Weiyang Li ◽  
Yu Zhang ◽  
Younan Xia
Keyword(s):  
Raman Scattering ◽  
Cancer Cells ◽  
Hot Spot ◽  
High Sensitivity ◽  
Silica Coating ◽  
Surface Enhanced Raman Scattering ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Enhanced Raman Scattering ◽  
Sers Tags

Surface-enhanced Raman scattering (SERS) tags have been actively explored as a multiplexing platform for sensitive detection of biomolecules. Here, we report a new type of SERS tags that was fabricated by sequentially functionalizing dimers made of 50 nm Ag nanospheres with 4-mercaptobenzoic acid as the Raman reporter molecule, silica coating as a protective shell and antibody as a targeting ligand. These dimer-based tags give highly enhanced and reproducible Raman signals owing to the presence of a well-defined SERS hot spot at the junction between two Ag nanospheres in the dimer. The SERS enhancement factor (EF) of an individual dimer tag supported on a glass slide can reach a level as high as 4.3 × 10 6 . In comparison, the EFs dropped to 2.8 × 10 5 and 8.7 × 10 5 , respectively, when Ag nanospheres and nanocubes with sizes similar to the spheres in the dimer were used to fabricate the tags using similar procedures. The SERS signals from aqueous suspensions of the dimer-based tags also showed high intensity and good stability. Potential use of the dimer-based tags was demonstrated by imaging cancer cells overexpressing HER2 receptors with good specificity and high sensitivity.

scholarly journals Single cell analysis using surface enhanced Raman scattering (SERS) tags

Methods ◽  
2012 ◽  
Vol 57 (3) ◽  
pp. 272-279 ◽  
Author(s):  
John P. Nolan ◽  
Erika Duggan ◽  
Er Liu ◽  
Danilo Condello ◽  
Isha Dave ◽  
...  
Keyword(s):  
Raman Scattering ◽  
Single Cell ◽  
Single Cell Analysis ◽  
Surface Enhanced Raman Scattering ◽  
Cell Analysis ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Enhanced Raman Scattering ◽  
Sers Tags

scholarly journals Designing Gold Nanoparticle-Ensembles as Surface Enhanced Raman Scattering Tags inside Human Retinal Cells

2012 ◽  
Vol 2012 ◽  
pp. 1-10 ◽  
Author(s):  
Sanda Boca ◽  
Dumitrita Rugina ◽  
Adela Pintea ◽  
Nicolae Leopold ◽  
Simion Astilean
Keyword(s):  
Raman Scattering ◽  
Surface Enhanced Raman Scattering ◽  
Plasmonic Nanostructures ◽  
Retinal Cells ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Active Nanoparticles ◽  
Enhanced Raman Scattering ◽  
Poly Ethylene ◽  
Sers Tags

Apart from the traditional development of surface-enhanced raman scattering (SERS) substrates for ultrasensitive spectroscopic analysis, an increasing interest is given nowadays to the design of the so-called SERS nanotags which integrate multiple SERS applications into single plasmonic nanoparticles. The fabrication of SERS tags is still a challenging task due to the complicated fabrication process. Typically, SERS tags are hybrid nanoconstructs consisting in a unique plasmonic nanoobject encoded with specific reporter molecules and enveloped in a protective shell that provides both biocompatibility and targeting function. Herein, we produce effective SERS tags consisting in small aggregates of gold nanoparticles (mainly dimers and trimers) which are captured from solution and then transferred into cells to perform as individual plasmonic nanostructures. Actually the small aggregates formed under controlled conditions are stabilized in solution by interlocking into a polymeric envelope made of thiol-modified poly(ethylene) glycol (PEG-SH). No further encoding operation is necessary in our case since part of ascorbic acid used as reducing agent remains attached in the interparticle junctions, providing persistent and strong SERS signal when the fabricated tags are internalized by human retinal cells. Our studies demonstrate a promising potential of new SERS-active nanoparticles to serve as effective reporters for biomedical tracing and imaging.

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