scholarly journals Single cell analysis using surface enhanced Raman scattering (SERS) tags

Methods ◽  
2012 ◽  
Vol 57 (3) ◽  
pp. 272-279 ◽  
Author(s):  
John P. Nolan ◽  
Erika Duggan ◽  
Er Liu ◽  
Danilo Condello ◽  
Isha Dave ◽  
...  
Keyword(s):  
Raman Scattering ◽  
Single Cell ◽  
Single Cell Analysis ◽  
Surface Enhanced Raman Scattering ◽  
Cell Analysis ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Enhanced Raman Scattering ◽  
Sers Tags

scholarly journals Surface-Enhanced Raman Scattering Based Microfluidics for Single-Cell Analysis

2018 ◽  
Vol 90 (20) ◽  
pp. 12004-12010 ◽  
Author(s):  
Marjorie R. Willner ◽  
Kay S. McMillan ◽  
Duncan Graham ◽  
Peter J. Vikesland ◽  
Michele Zagnoni
Keyword(s):  
Raman Scattering ◽  
Single Cell ◽  
Single Cell Analysis ◽  
Surface Enhanced Raman Scattering ◽  
Cell Analysis ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Enhanced Raman Scattering

scholarly journals Correlation of surface-enhanced Raman scattering (SERS) with the surface density of gold nanoparticles: evaluation of the critical number of SERS tags for a detectable signal

2019 ◽  
Vol 10 ◽  
pp. 1016-1023 ◽  
Author(s):  
Vincenzo Amendola
Keyword(s):  
Gold Nanoparticles ◽  
Raman Scattering ◽  
Surface Density ◽  
Surface Enhanced Raman Scattering ◽  
Operating Conditions ◽  
Acquisition Time ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Enhanced Raman Scattering ◽  
Sers Tags

The use of plasmonic nanotags based on the surface-enhanced Raman scattering (SERS) effect is highly promising for several applications in analytical chemistry, biotechnological assays and nanomedicine. To this end, a crucial parameter is the minimum number of SERS tags that allows for the collection of intense Raman signals under real operating conditions. Here, SERS Au nanotags (AuNTs) based on clustered gold nanoparticles are deposited on a substrate and analyzed in the same region using Raman spectroscopy and transmission electron microscopy. In this way, the Raman spectra and the surface density of the SERS tags are correlated directly, showing that 1 tag/µm2 is enough to generate an intense signal above the noise level at 633 nm with an excitation power of only 0.65 mW and an acquisition time of just 1 s with a 50× objective. The AuNT density can be even lower than 1 tag/µm2 when the acquisition time is extended to 10 s, but must be increased to 3 tags/µm2 when a 20× objective is employed under the same excitation conditions. In addition, in order to observe a linear response, it was found that 10 SERS AuNTs inside the probed area are required. These findings indicate that a better signal-to-noise ratio requires high-magnification optics, while linearity versus tag number can be improved by using low-magnification optics or a high tag density. In general the suitability of plasmonic SERS labels for ultrasensitive analytical and biomedical applications is evident.

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