scholarly journals Dually degradable click hydrogels for controlled degradation and protein release

2014 ◽  
Vol 2 (34) ◽  
pp. 5511-5521 ◽  
Author(s):  
Prathamesh M. Kharkar ◽  
April M. Kloxin ◽  
Kristi L. Kiick
Keyword(s):  
Controlled Release ◽  
Bond Cleavage ◽  
Ester Hydrolysis ◽  
Protein Release

Crosslinks that can undergo click bond cleavage and ester hydrolysis were incorporated to design glutathione-sensitive, dually degradable hydrogels for degradation-mediated, controlled release of cargo molecules.

scholarly journals A novel mechanism of enzymic ester hydrolysis. Inversion of configuration and carbon-oxygen bond cleavage by secondary alkylsulphohydrolases from detergent-degrading micro-organisms

1977 ◽  
Vol 165 (3) ◽  
pp. 575-580 ◽  
Author(s):  
B Bartholomew ◽  
K S Dodgson ◽  
G W J Matcham ◽  
D J Shaw ◽  
G F White
Keyword(s):  
Bond Cleavage ◽  
Hydrolytic Enzymes ◽  
Ester Hydrolysis ◽  
Enzymic Hydrolysis ◽  
Hydrolysis Products ◽  
Bond Scission ◽  
Micro Organisms ◽  
Hydrolysis Of ◽  
Oxygen Bond ◽  
Comamonas Terrigena

The hydrolysis was studied of potassium (+)-octan-2-yl sulphate by two analogous, optically stereospecific, secondary alkylsulphohydrolases purified from two detergent-degrading micro-organisms, Comamonas terrigena and Pseudomonas C12B. Polarimetry studies have shown that (+)-octan-2-yl sulphate prepared from (+)-octan-2-ol is hydrolysed by both enzymes to yield (-)-octan-2-ol. This inversion of configuration implies that the enzymes are catalysing the scission of the C-O bond of the C-O-S linkage, a type of bond scission apparently not hitherto encountered among hydrolytic enzymes acting on ester bonds. Enzymic hydrolysis of potassium (+)-octan-2-yl sulphate in the presence of H218O and analysis of hydrolysis products for the presence of 18O has confirmed that C-O bond scission (and not O-S bond scission) occurs with both enzymes.

Lysozyme Release from Lipid-Based Implants

2014 ◽  
Vol 1060 ◽  
pp. 83-86
Author(s):  
Joana Portugal Mota ◽  
Alexandre Campos ◽  
Duangratana Shuwisitkul ◽  
Nuno Saraiva ◽  
Marisa Nicolai
Keyword(s):  
Controlled Release ◽  
Drug Release ◽  
Smooth Surface ◽  
Surface Protein ◽  
Protein Release ◽  
Protein Loading ◽  
Loading Efficiency ◽  
Model Protein ◽  
Preparation Techniques ◽  
Potential Use

To better understand lipid-based implants applied to proteins, lysozyme was used as a model protein and was loaded into lipid-based implants in different contents. Cylindrical and spherical implants loaded with lysozyme presented a smooth surface. Protein loading efficiency decreased with increasing lysozyme loading. Lysozyme kept its activity after different implant preparation techniques. Drug release tremendously increased by increasing protein loading from 8.9 to 13.7% (w/w), for cylindrical implants. Lysozyme release was faster from cylindrical implants when compared with the spherical ones. Furthermore, by increasing the hydrophobicity of the lipid, protein release decreased. This study demonstrates the potential use of lipid-based implants for the controlled release of proteins.

Photothermal triggered protein release from an injectable polycaprolactone-based microspherical depot

2017 ◽  
Vol 5 (20) ◽  
pp. 3634-3639
Author(s):  
Chunling Ge ◽  
Johan S. Basuki ◽  
Jacinta White ◽  
Ruixia Hou ◽  
Yong Peng ◽  
...  
Keyword(s):  
Controlled Release ◽  
Visible Light ◽  
Protein Release ◽  
Biologically Active

Visible light mediated controlled release of biologically active enzymes was confirmed by released horseradish peroxidase's ability to ameliorate H2O2 cytotoxicity in vitro.

Potential Controlled-Release Herbicides from 2,4-D Esters of Starches

Weed Science ◽  
1974 ◽  
Vol 22 (5) ◽  
pp. 415-418 ◽  
Author(s):  
C. L. Mehltretter ◽  
W. B. Roth ◽  
F. B. Weakley ◽  
T. A. McGuire ◽  
C. R. Russell
Keyword(s):  
Acetic Acid ◽  
Controlled Release ◽  
Ambient Temperature ◽  
Bond Cleavage ◽  
Dichlorophenoxy Acetic Acid ◽  
Acetic Acid Esters ◽  
Acid Esters

Unmodified, cyanoethylated, and crosslinked corn starches were reacted with 2,4-dichlorophenoxyacetyl chloride in pyridine to produce the corresponding 2,4-D [(2,4-dichlorophenoxy)acetic acid]esters of high acyl content. Hydrolysis studies at pH 6 and 8 at ambient temperature showed that these insoluble compositions liberated varying amounts of 2,4-D and soluble 2,4-D esters by ester link and glucoside bond cleavage, respectively, at rates that indicate their possible use as controlled-release herbicides.

Semisolid, Self-Catalyzed Poly(Ortho Ester)s As Controlled-Release Systems: Protein Release and Protein Stability Issues

2002 ◽  
Vol 91 (4) ◽  
pp. 1065-1074 ◽  
Author(s):  
Marco van de Weert ◽  
Marinus J. Van Steenbergen ◽  
Jeffrey L. Cleland ◽  
Jorge Heller ◽  
Wim E. Hennink ◽  
...  
Keyword(s):  
Controlled Release ◽  
Protein Stability ◽  
Protein Release ◽  
Ortho Ester ◽  
Stability Issues

scholarly journals Design of biodegradable bi-compartmental microneedles for the stabilization and the controlled release of the labile molecule collagenase for skin healthcare

2021 ◽  
Author(s):  
Concetta Di Natale ◽  
Domenico De Rosa ◽  
Martina Profeta ◽  
Rezvan Jamaledin ◽  
Alessandro Attanasio ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Controlled Release ◽  
Drug Delivery System ◽  
Delivery System ◽  
Protein Release ◽  
Labile Molecule

Polymeric microneedles (MNs) have emerged as a novel class of drug delivery system thanks to their ability in penetrating the skin with no pain, encapsulate active proteins and in particular, proposed bicompartimental MNs can tune protein release.

scholarly journals Tunable delayed controlled release profile from layered polymeric microparticles

2017 ◽  
Vol 5 (23) ◽  
pp. 4487-4498 ◽  
Author(s):  
D. Dutta ◽  
C. Fauer ◽  
K. Hickey ◽  
M. Salifu ◽  
S. E. Stabenfeldt
Keyword(s):  
Controlled Release ◽  
Solvent Evaporation ◽  
Release Profile ◽  
Protein Release ◽  
Protein Distribution ◽  
Ethanol Content ◽  
Polymeric Microparticles ◽  
Release Profiles

Solvent evaporation parameters and ethanol content during PLGA/PLLA microparticle (MP) fabrication affect protein distribution and MP structure, thereby altering the protein release profiles.

Tetrazines-Mediated Bioorthogonal Removal of 3-Isocyanopropyl Groups Enables the Controlled Release of Nitric Oxide In Vivo

2021 ◽  
Author(s):  
Jianbing Wu ◽  
Tao Sun ◽  
Chenxi Yang ◽  
Tian Lv ◽  
Yu-Yang Bi ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Free Radical ◽  
Controlled Release ◽  
Bond Cleavage ◽  
Bioorthogonal Chemistry

Bond cleavage bioorthogonal chemistry has been widely employed to restore or activate proteins or prodrugs. Nitric Oxide (NO), as a free radical molecule, has joined the clinical arena of cancer...

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