Semisolid, Self-Catalyzed Poly(Ortho Ester)s As Controlled-Release Systems: Protein Release and Protein Stability Issues

2002 ◽  
Vol 91 (4) ◽  
pp. 1065-1074 ◽  
Author(s):  
Marco van de Weert ◽  
Marinus J. Van Steenbergen ◽  
Jeffrey L. Cleland ◽  
Jorge Heller ◽  
Wim E. Hennink ◽  
...  
Keyword(s):  
Controlled Release ◽  
Protein Stability ◽  
Protein Release ◽  
Ortho Ester ◽  
Stability Issues

Protein stability in controlled-release systems

10.1038/71875 ◽  
2000 ◽  
Vol 18 (1) ◽  
pp. 24-25 ◽  
Author(s):  
K. Fu ◽  
A.M. Klibanov ◽  
R. Langer
Keyword(s):  
Controlled Release ◽  
Protein Stability

scholarly journals Protein stability: a crystallographer's perspective

2016 ◽  
Vol 72 (2) ◽  
pp. 72-95 ◽  
Author(s):  
Marc C. Deller ◽  
Leopold Kong ◽  
Bernhard Rupp
Keyword(s):  
Protein Stability ◽  
Conformational Stability ◽  
Factors Affecting ◽  
Food Industries ◽  
Compositional Stability ◽  
Major Interest ◽  
Protein Conformational Stability ◽  
Academic Researchers ◽  
Stability Issues ◽  
Practical Level

Protein stability is a topic of major interest for the biotechnology, pharmaceutical and food industries, in addition to being a daily consideration for academic researchers studying proteins. An understanding of protein stability is essential for optimizing the expression, purification, formulation, storage and structural studies of proteins. In this review, discussion will focus on factors affecting protein stability, on a somewhat practical level, particularly from the view of a protein crystallographer. The differences between protein conformational stability and protein compositional stability will be discussed, along with a brief introduction to key methods useful for analyzing protein stability. Finally, tactics for addressing protein-stability issues during protein expression, purification and crystallization will be discussed.

Protein release from poly(ortho ester) extruded rods

2001 ◽  
Vol 172 (1) ◽  
pp. 67-72
Author(s):  
Alexandra Rothen-Weinhold ◽  
John Barr ◽  
Steven Y. Ng ◽  
Hui-Rong Shen ◽  
Robert Gurny ◽  
...  
Keyword(s):  
Protein Release ◽  
Ortho Ester

Lysozyme Release from Lipid-Based Implants

2014 ◽  
Vol 1060 ◽  
pp. 83-86
Author(s):  
Joana Portugal Mota ◽  
Alexandre Campos ◽  
Duangratana Shuwisitkul ◽  
Nuno Saraiva ◽  
Marisa Nicolai
Keyword(s):  
Controlled Release ◽  
Drug Release ◽  
Smooth Surface ◽  
Surface Protein ◽  
Protein Release ◽  
Protein Loading ◽  
Loading Efficiency ◽  
Model Protein ◽  
Preparation Techniques ◽  
Potential Use

To better understand lipid-based implants applied to proteins, lysozyme was used as a model protein and was loaded into lipid-based implants in different contents. Cylindrical and spherical implants loaded with lysozyme presented a smooth surface. Protein loading efficiency decreased with increasing lysozyme loading. Lysozyme kept its activity after different implant preparation techniques. Drug release tremendously increased by increasing protein loading from 8.9 to 13.7% (w/w), for cylindrical implants. Lysozyme release was faster from cylindrical implants when compared with the spherical ones. Furthermore, by increasing the hydrophobicity of the lipid, protein release decreased. This study demonstrates the potential use of lipid-based implants for the controlled release of proteins.

Improving Protein Stability and Controlling Protein Release by Adding Poly (Cyclohexane -1, 4 -Diyl Acetone Dimethylene Ketal) to PLGA Microspheres

2015 ◽  
Vol 12 (6) ◽  
pp. 726-735 ◽  
Author(s):  
Chenhui Wang ◽  
Changhui Yu ◽  
Kongtong Yu ◽  
Lesheng Teng ◽  
Jiaxin Liu ◽  
...  
Keyword(s):  
Protein Stability ◽  
Protein Release ◽  
Plga Microspheres

Controlled release from erod1ble poly(ortho ester) drug delivery systems

1984 ◽  
Vol 1 (1) ◽  
pp. 23-32 ◽  
Author(s):  
Randall V. Sparer ◽  
Shih Chung ◽  
Cheryl D. Ringeisen ◽  
Kenneth J. Himmelstein
Keyword(s):  
Drug Delivery ◽  
Controlled Release ◽  
Drug Delivery Systems ◽  
Delivery Systems ◽  
Ortho Ester

Quality Control, Preparation, and Protein Stability Issues for Blood Serum and Plasma Used In Biomarker Discovery and Proteomic Profiling Assays

2004 ◽  
Vol 3 (4) ◽  
pp. 45-50 ◽  
Author(s):  
Richard Drake ◽  
Lisa Cazares ◽  
Alberto Corsica ◽  
Gunjan Malik ◽  
Ellen Schwegler ◽  
...  
Keyword(s):  
Quality Control ◽  
Blood Serum ◽  
Protein Stability ◽  
Biomarker Discovery ◽  
Proteomic Profiling ◽  
Stability Issues

scholarly journals Dually degradable click hydrogels for controlled degradation and protein release

2014 ◽  
Vol 2 (34) ◽  
pp. 5511-5521 ◽  
Author(s):  
Prathamesh M. Kharkar ◽  
April M. Kloxin ◽  
Kristi L. Kiick
Keyword(s):  
Controlled Release ◽  
Bond Cleavage ◽  
Ester Hydrolysis ◽  
Protein Release

Crosslinks that can undergo click bond cleavage and ester hydrolysis were incorporated to design glutathione-sensitive, dually degradable hydrogels for degradation-mediated, controlled release of cargo molecules.

Photothermal triggered protein release from an injectable polycaprolactone-based microspherical depot

2017 ◽  
Vol 5 (20) ◽  
pp. 3634-3639
Author(s):  
Chunling Ge ◽  
Johan S. Basuki ◽  
Jacinta White ◽  
Ruixia Hou ◽  
Yong Peng ◽  
...  
Keyword(s):  
Controlled Release ◽  
Visible Light ◽  
Protein Release ◽  
Biologically Active

Visible light mediated controlled release of biologically active enzymes was confirmed by released horseradish peroxidase's ability to ameliorate H2O2 cytotoxicity in vitro.

Sign in / Sign up

Export Citation Format

Share Document