Fabrication of ZnO nanoplates for visible light-induced imaging of living cells

2014 ◽  
Vol 2 (16) ◽  
pp. 2311-2317 ◽  
Author(s):  
Jooran Lee ◽  
Joon Sig Choi ◽  
Minjoong Yoon
Keyword(s):  
Visible Light ◽  
Hela Cells ◽  
Fluorescence Emission ◽  
Green Emission ◽  
Living Cells ◽  
Cellular Imaging ◽  
Red Fluorescence ◽  
Light Excitation

APTES-modified ZnO nanoplates (NPls) showed excellent permeability into HeLa cells with negligible cytotoxicity, exhibiting strong red fluorescence emission (∼650 nm) under visible light excitation at 405 nm. Therefore, the synthesized ZnO NPls would be useful for highly resolved cellular imaging by avoiding the overlap with the cellular intrinsic green emission.

Design of Ratiometric Emission Probe with Visible Light Excitation for Determination of Ca2+in Living Cells

2014 ◽  
Vol 86 (16) ◽  
pp. 8025-8030 ◽  
Author(s):  
Qiaoling Liu ◽  
Huizhi Du ◽  
Xiaoze Ren ◽  
Wei Bian ◽  
Li Fan ◽  
...  
Keyword(s):  
Visible Light ◽  
Living Cells ◽  
Light Excitation

Use of fluorescence-based sensors to determine the nitrogen status of paddy rice

2013 ◽  
Vol 151 (6) ◽  
pp. 862-871 ◽  
Author(s):  
J. W. LI ◽  
J. X. ZHANG ◽  
Z. ZHAO ◽  
X. D. LEI ◽  
X. L. XU ◽  
...  
Keyword(s):  
Environmental Concern ◽  
Red Light ◽  
Fluorescence Emission ◽  
Ultra Violet ◽  
Fertilizer Application ◽  
Red Fluorescence ◽  
Rice Leaf ◽  
Plant Densities ◽  
Light Excitation ◽  
N Status

SUMMARYThe environmental concern about diffuse pollution from nitrogen (N) fertilizers has led to increased research on the diagnosis of crop N status. The SPAD chlorophyll (Chl) meter is the most commonly used tool for rice (Oryza sativa L.) N status diagnosis, but measurements are conducted at a specific point and readings are affected by different leaf positions. Many measurements per plant must be taken in order to increase the accuracy of N status diagnosis, which limits its application. The present paper attempts to determine rice N status at the canopy level using Multiplex®, a new hand-held optical fluorescence sensor. The fluorescence emission of rice leaves under light excitation was utilized by Multiplex® to non-destructively assess rice leaf Chl and phenolic compound content. A field experiment was conducted in 2011 using a completely randomized split-plot design, with main-plot treatments being six N fertilizer application rates and subplot treatments being different plant densities. Leaf Chl and phenolic compounds were evaluated using the ratio of far-red fluorescence (FRF) to red fluorescence (RF) emission under red light excitation (simple fluorescence ratio, SFR_R) (R2 = 0·35, P < 0·01) and the ratio of decadic logarithm of red to ultra-violet (UV) fluorescence emission (R2 = 0·30, P < 0·01), respectively. Both SPAD reading and fluorescence-based indices including flavonoids (FLAV), nitrogen balance index (NBI_R) and SFR_R could be used to predict rice leaf N contents. The canopy FLAV, SFR_R and NBI_R were all highly correlated to average SPAD readings (R2 > 0·70 in most cases, P < 0·01). Therefore, Multiplex® can be used as an alternative to SPAD to determine rice N status in paddy fields.

scholarly journals Elemental composition control of gold-titania nanocomposites by site-specific mineralization using artificial peptides and DNA

2021 ◽  
Vol 4 (1) ◽  
Author(s):  
Makoto Ozaki ◽  
Takahito Imai ◽  
Takaaki Tsuruoka ◽  
Shungo Sakashita ◽  
Kin-ya Tomizaki ◽  
...  
Keyword(s):  
Visible Light ◽  
Elemental Composition ◽  
Inorganic Compounds ◽  
Medical Science ◽  
Induction Effect ◽  
Site Specific ◽  
Composition Control ◽  
Light Excitation ◽  
Titania Photocatalyst ◽  
Scale Control

AbstractBiomineralization, the precipitation of various inorganic compounds in biological systems, can be regulated in terms of the size, morphology, and crystal structure of these compounds by biomolecules such as proteins and peptides. However, it is difficult to construct complex inorganic nanostructures because they precipitate randomly in solution. Here, we report that the elemental composition of inorganic nanocomposites can be controlled by site-specific mineralization by changing the number of two inorganic-precipitating peptides bound to DNA. With a focus on gold and titania, we constructed a gold-titania photocatalyst that responds to visible light excitation. Both microscale and macroscale observations revealed that the elemental composition of this gold-titania nanocomposite can be controlled in several ten nm by changing the DNA length and the number of peptide binding sites on the DNA. Furthermore, photocatalytic activity and cell death induction effect under visible light (>450 nm) irradiation of the manufactured gold-titania nanocomposite was higher than that of commercial gold-titania and titania. Thus, we have succeeded in forming titania precipitates on a DNA terminus and gold precipitates site-specifically on double-stranded DNA as intended. Such nanometer-scale control of biomineralization represent a powerful and efficient tool for use in nanotechnology, electronics, ecology, medical science, and biotechnology.

Preparation of Visible-Light-Responsive TiO2-xNx Photocatalyst by a Very Simple Method

2011 ◽  
Vol 383-390 ◽  
pp. 3188-3191
Author(s):  
Han Jie Huang ◽  
Wen Long She ◽  
Ling Wen Yang ◽  
Hai Peng Huang
Keyword(s):  
Photocatalytic Activity ◽  
Visible Light ◽  
Photoelectron Spectroscopy ◽  
Diffuse Reflection ◽  
Ammonia Solution ◽  
Reflection Spectra ◽  
X Ray Diffraction ◽  
Simple Method ◽  
X Ray ◽  
Light Excitation

A visible-light-responsive TiO2-xNx photocatalyst was prepared by a very simple method. Ammonia solution was used as nitrogen resource in this paper. The TiO2-xNx photocatalyst was characterized by X-ray diffraction (XRD), Brunauer-Emmett-Teller (BET), UV-Vis diffuse reflection spectra (DRS), and X-ray photoelectron spectroscopy (XPS). The ethylene was selected as a target pollutant under visible light excitation to evaluate the activity of this photocatalyst. The new prepared TiO2-xNx photocatalyst with strong photocatalytic activity under visible light irradiation was demonstrated in the experiment.

Syntheses, crystal structures and photoluminescence properties of two rare-earth molybdates CsLn(MoO4)2 (Ln=Eu, Tb)

2018 ◽  
Vol 233 (2) ◽  
pp. 73-79 ◽  
Author(s):  
Dan Zhao ◽  
Fa-Xue Ma ◽  
Bao-Zhong Liu ◽  
Yun-Chang Fan ◽  
Xue-Feng Han ◽  
...  
Keyword(s):  
Rare Earth ◽  
Uv Light ◽  
Layer Structure ◽  
Green Emission ◽  
Salt Flux ◽  
Photoluminescence Properties ◽  
X Ray Diffraction ◽  
Orthorhombic Space Group ◽  
Light Excitation ◽  
Rare Earth Molybdates

AbstractSingle crystals of two cesium rare-earth molybdates CsLn(MoO4)2(Ln=Eu, Tb) have been prepared using the high temperature molten salt (flux) method. Single-crystal X-ray diffraction analyses reveal that they crystallize in the orthorhombic space groupPccm(No. 49) and features a 2D layer structure that is composed of [Ln(MoO4)2]∞and [Cs]∞layers. Under near-UV light excitation, emission spectrum of CsEu(MoO4)2consists of several sharp lines due to the characteristic electronic transitions of Eu3+ions, whereas CsTb(MoO4)2exhibits characteristic green emission of Tb3+ions.

scholarly journals Effects of monoolein-based cubosome formulations on lipid droplets and mitochondria of HeLa cells

2015 ◽  
Vol 4 (4) ◽  
pp. 1025-1036 ◽  
Author(s):  
Angela Maria Falchi ◽  
Antonella Rosa ◽  
Angela Atzeri ◽  
Alessandra Incani ◽  
Sandrina Lampis ◽  
...  
Keyword(s):  
Hela Cells ◽  
Lipid Droplets ◽  
Living Cells

Analysis of living cells after staining with organelle-specific dyes shows that monoolein-based cubosome treatment induces accumulation of lipid droplets (green) and mitochondrial (red) hyperpolarization.

A novel chemosensor with visible light excitability for sensing CN− in aqueous medium and living cells via a Cu2+ displacement approach

2017 ◽  
Vol 9 (2) ◽  
pp. 259-266 ◽  
Author(s):  
Liyuan Qin ◽  
Lingjie Hou ◽  
Jia Feng ◽  
Jianbin Chao ◽  
Yu Wang ◽  
...  
Keyword(s):  
Visible Light ◽  
Aqueous Medium ◽  
Living Cells ◽  
Receptor System ◽  
System L ◽  
Displacement Approach

A new and simple julolidine based molecular receptor system L linked to 4-phenylsemicarbazide through an imine moiety has been synthesized and characterized.

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