scholarly journals Effects of monoolein-based cubosome formulations on lipid droplets and mitochondria of HeLa cells

2015 ◽  
Vol 4 (4) ◽  
pp. 1025-1036 ◽  
Author(s):  
Angela Maria Falchi ◽  
Antonella Rosa ◽  
Angela Atzeri ◽  
Alessandra Incani ◽  
Sandrina Lampis ◽  
...  
Keyword(s):  
Hela Cells ◽  
Lipid Droplets ◽  
Living Cells

Analysis of living cells after staining with organelle-specific dyes shows that monoolein-based cubosome treatment induces accumulation of lipid droplets (green) and mitochondrial (red) hyperpolarization.

scholarly journals Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system

2018 ◽  
Author(s):  
Yuriko Kakimoto ◽  
Shinya Tashiro ◽  
Rieko Kojima ◽  
Yuuki Morozumi ◽  
Toshiya Endo ◽  
...  
Keyword(s):  
Hela Cells ◽  
Mammalian Cells ◽  
Lipid Droplets ◽  
Living Cells ◽  
Yeast Cells ◽  
Advantages And Disadvantages ◽  
Contact Sites ◽  
Potential Tool ◽  
Rule Out

Functional integrity of eukaryotic organelles relies on direct physical contacts between distinct organelles. However, the entity of organelle-tethering factors is not well understood due to lack of means to analyze inter-organelle interactions in living cells. Here we evaluate the split-GFP system for visualizing organelle contact sites in vivo and show its advantages and disadvantages. We observed punctate GFP signals from the split-GFP fragments targeted to any pairs of organelles among the ER, mitochondria, peroxisomes, vacuole and lipid droplets in yeast cells, which suggests that these organelles form contact sites with multiple organelles simultaneously although it is difficult to rule out the possibilities that these organelle contacts sites are artificially formed by the irreversible associations of the split-GFP probes. Importantly, split-GFP signals in the overlapped regions of the ER and mitochondria were mainly co-localized with ERMES, an authentic ER-mitochondria tethering structure, suggesting that split-GFP assembly depends on the preexisting inter-organelle contact sites. We also confirmed that the split-GFP system can be applied to detection of the ER-mitochondria contact sites in HeLa cells. We thus propose that the split-GFP system is a potential tool to observe and analyze inter-organelle contact sites in living yeast and mammalian cells.

A Molecular Logic Gate Enables Super-Resolved Imaging of Intracellular Lipid Droplets

2019 ◽  
Author(s):  
Adam Eördögh ◽  
Carolina Paganini ◽  
Dorothea Pinotsi ◽  
Paolo Arosio ◽  
Pablo Rivera-Fuentes
Keyword(s):  
Single Molecule ◽  
Lipid Droplets ◽  
Neutral Lipids ◽  
Logic Gate ◽  
Living Cells ◽  
Three Dimensions ◽  
Single Molecule Imaging ◽  
Molecular Logic Gate ◽  
Molecular Logic ◽  
Cellular Compartments

<div>Photoactivatable dyes enable single-molecule imaging in biology. Despite progress in the development of new fluorophores and labeling strategies, many cellular compartments remain difficult to image beyond the limit of diffraction in living cells. For example, lipid droplets, which are organelles that contain mostly neutral lipids, have eluded single-molecule imaging. To visualize these challenging subcellular targets, it is necessary to develop new fluorescent molecular devices beyond simple on/off switches. Here, we report a fluorogenic molecular logic gate that can be used to image single molecules associated with lipid droplets with excellent specificity. This probe requires the subsequent action of light, a lipophilic environment and a competent nucleophile to produce a fluorescent product. The combination of these requirements results in a probe that can be used to image the boundary of lipid droplets in three dimensions with resolutions beyond the limit of diffraction. Moreover, this probe enables single-molecule tracking of lipids within and between droplets in living cells.</div>

A two-photon fluorescent probe for imaging of endogenous formaldehyde in HeLa cells and quantitative detection of basal formaldehyde in milk samples

2019 ◽  
Vol 11 (23) ◽  
pp. 2969-2975 ◽  
Author(s):  
Fangyun Xin ◽  
Yong Tian ◽  
Jing Jing ◽  
Xiaoling Zhang
Keyword(s):  
Fluorescent Probe ◽  
Hela Cells ◽  
Living Cells ◽  
Quantitative Detection ◽  
Two Photon ◽  
Milk Samples

A fluorescent probe NaP which can image endogenous formaldehyde in living cells and quantitatively detect basal formaldehyde in milk samples.

Aurone Derivative Revealing the Metabolism of Lipid Droplets and Monitoring Oxidative Stress in Living Cells

2020 ◽  
Vol 92 (9) ◽  
pp. 6631-6636 ◽  
Author(s):  
Kangnan Wang ◽  
Shuyue Ma ◽  
Yanyan Ma ◽  
Yuping Zhao ◽  
Miaomiao Xing ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Lipid Droplets ◽  
Living Cells

scholarly journals Visualization of ATP levels inside single living cells with fluorescence resonance energy transfer-based genetically encoded indicators

2009 ◽  
Vol 106 (37) ◽  
pp. 15651-15656 ◽  
Author(s):  
Hiromi Imamura ◽  
Kim P. Huynh Nhat ◽  
Hiroko Togawa ◽  
Kenta Saito ◽  
Ryota Iino ◽  
...  
Keyword(s):  
Energy Transfer ◽  
Fluorescence Resonance Energy Transfer ◽  
Atp Synthase ◽  
Hela Cells ◽  
Dissociation Constants ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Living Cells ◽  
Atp Levels ◽  
Fluorescence Resonance

Adenosine 5′-triphosphate (ATP) is the major energy currency of cells and is involved in many cellular processes. However, there is no method for real-time monitoring of ATP levels inside individual living cells. To visualize ATP levels, we generated a series of fluorescence resonance energy transfer (FRET)-based indicators for ATP that were composed of the ε subunit of the bacterial FoF1-ATP synthase sandwiched by the cyan- and yellow-fluorescent proteins. The indicators, named ATeams, had apparent dissociation constants for ATP ranging from 7.4 μM to 3.3 mM. By targeting ATeams to different subcellular compartments, we unexpectedly found that ATP levels in the mitochondrial matrix of HeLa cells are significantly lower than those of cytoplasm and nucleus. We also succeeded in measuring changes in the ATP level inside single HeLa cells after treatment with inhibitors of glycolysis and/or oxidative phosphorylation, revealing that glycolysis is the major ATP-generating pathway of the cells grown in glucose-rich medium. This was also confirmed by an experiment using oligomycin A, an inhibitor of FoF1-ATP synthase. In addition, it was demonstrated that HeLa cells change ATP-generating pathway in response to changes of nutrition in the environment.

Fabrication of ZnO nanoplates for visible light-induced imaging of living cells

2014 ◽  
Vol 2 (16) ◽  
pp. 2311-2317 ◽  
Author(s):  
Jooran Lee ◽  
Joon Sig Choi ◽  
Minjoong Yoon
Keyword(s):  
Visible Light ◽  
Hela Cells ◽  
Fluorescence Emission ◽  
Green Emission ◽  
Living Cells ◽  
Cellular Imaging ◽  
Red Fluorescence ◽  
Light Excitation

APTES-modified ZnO nanoplates (NPls) showed excellent permeability into HeLa cells with negligible cytotoxicity, exhibiting strong red fluorescence emission (∼650 nm) under visible light excitation at 405 nm. Therefore, the synthesized ZnO NPls would be useful for highly resolved cellular imaging by avoiding the overlap with the cellular intrinsic green emission.

A turn-on fluorescent probe for tumor hypoxia imaging in living cells

2015 ◽  
Vol 51 (79) ◽  
pp. 14739-14741 ◽  
Author(s):  
Qi Cai ◽  
Tao Yu ◽  
Weiping Zhu ◽  
Yufang Xu ◽  
Xuhong Qian
Keyword(s):  
Fluorescent Probe ◽  
Hela Cells ◽  
Tumor Hypoxia ◽  
Living Cells ◽  
Hypoxia Imaging ◽  
Turn On ◽  
Reducing Ability ◽  
Oxygen Concentrations

A turn-on fluorescent probe was designed and synthesized for tumor hypoxia imaging, which could show excellent reducing ability under chemical or bio-reductase conditions and good performance in hypoxia imaging for HeLa cells at different oxygen concentrations.

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