Design of Ratiometric Emission Probe with Visible Light Excitation for Determination of Ca2+in Living Cells

2014 ◽  
Vol 86 (16) ◽  
pp. 8025-8030 ◽  
Author(s):  
Qiaoling Liu ◽  
Huizhi Du ◽  
Xiaoze Ren ◽  
Wei Bian ◽  
Li Fan ◽  
...  
2014 ◽  
Vol 2 (16) ◽  
pp. 2311-2317 ◽  
Author(s):  
Jooran Lee ◽  
Joon Sig Choi ◽  
Minjoong Yoon

APTES-modified ZnO nanoplates (NPls) showed excellent permeability into HeLa cells with negligible cytotoxicity, exhibiting strong red fluorescence emission (∼650 nm) under visible light excitation at 405 nm. Therefore, the synthesized ZnO NPls would be useful for highly resolved cellular imaging by avoiding the overlap with the cellular intrinsic green emission.


2019 ◽  
Vol 11 (35) ◽  
pp. 4440-4449
Author(s):  
Chandana Sen ◽  
Sunanda Dey ◽  
Chiranjit Patra ◽  
Debashis Mallick ◽  
Chittaranjan Sinha

Quinolinyl-azo-naphthol (HL) is a selective turn-on chemosensor for Al3+ in the presence of other ions and the excitation at visible light (537 nm) shows a 750 fold enhancement of emission at 612 nm.


2021 ◽  
Author(s):  
Lijuan Liu ◽  
Shengting Zhang ◽  
Xiaodan Zheng ◽  
Hongmei Li ◽  
Qi Chen ◽  
...  

Fusobacterium nucleatum has been employed for the first time to synthesize fluorescent carbon dots which could be applied for the determination of Fe3+ ions in living cells and bioimaging in vitro and in vivo with excellent biocompatibility.


2021 ◽  
Vol 4 (1) ◽  
Author(s):  
Makoto Ozaki ◽  
Takahito Imai ◽  
Takaaki Tsuruoka ◽  
Shungo Sakashita ◽  
Kin-ya Tomizaki ◽  
...  

AbstractBiomineralization, the precipitation of various inorganic compounds in biological systems, can be regulated in terms of the size, morphology, and crystal structure of these compounds by biomolecules such as proteins and peptides. However, it is difficult to construct complex inorganic nanostructures because they precipitate randomly in solution. Here, we report that the elemental composition of inorganic nanocomposites can be controlled by site-specific mineralization by changing the number of two inorganic-precipitating peptides bound to DNA. With a focus on gold and titania, we constructed a gold-titania photocatalyst that responds to visible light excitation. Both microscale and macroscale observations revealed that the elemental composition of this gold-titania nanocomposite can be controlled in several ten nm by changing the DNA length and the number of peptide binding sites on the DNA. Furthermore, photocatalytic activity and cell death induction effect under visible light (>450 nm) irradiation of the manufactured gold-titania nanocomposite was higher than that of commercial gold-titania and titania. Thus, we have succeeded in forming titania precipitates on a DNA terminus and gold precipitates site-specifically on double-stranded DNA as intended. Such nanometer-scale control of biomineralization represent a powerful and efficient tool for use in nanotechnology, electronics, ecology, medical science, and biotechnology.


2011 ◽  
Vol 383-390 ◽  
pp. 3188-3191
Author(s):  
Han Jie Huang ◽  
Wen Long She ◽  
Ling Wen Yang ◽  
Hai Peng Huang

A visible-light-responsive TiO2-xNx photocatalyst was prepared by a very simple method. Ammonia solution was used as nitrogen resource in this paper. The TiO2-xNx photocatalyst was characterized by X-ray diffraction (XRD), Brunauer-Emmett-Teller (BET), UV-Vis diffuse reflection spectra (DRS), and X-ray photoelectron spectroscopy (XPS). The ethylene was selected as a target pollutant under visible light excitation to evaluate the activity of this photocatalyst. The new prepared TiO2-xNx photocatalyst with strong photocatalytic activity under visible light irradiation was demonstrated in the experiment.


2016 ◽  
Vol 184 (2) ◽  
pp. 603-610 ◽  
Author(s):  
Man Li ◽  
Hongfang Gao ◽  
Xiaofei Wang ◽  
Yufeng Wang ◽  
Honglan Qi ◽  
...  

1990 ◽  
Vol 112 (3) ◽  
pp. 283-294 ◽  
Author(s):  
G. I. Zahalak ◽  
W. B. McConnaughey ◽  
E. L. Elson

In this paper we review the cell-poking technique as an approach for investigating the mechanical properties of living cells. We first summarize the rationale for the technique and the mainly qualitative results obtained so far. Then we provide a technical description of the instrument as it is configured at present. This is followed by a discussion of the current status of analytical results available for interpreting cell-poking measurements. In the final section we apply these results to an analysis of unmodulated and modulated lymphocytes and neutrophils, and conclude that the mechanical response of these leukocytes to indentation is not consistent with simple models developed by previous investigators on the basis of micropipette-aspiration experiments.


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