Graphene oxide for fluorescence-mediated enzymatic activity assays

2014 ◽  
Vol 2 (17) ◽  
pp. 2452 ◽  
Author(s):  
Hongje Jang ◽  
Jieon Lee ◽  
Dal-Hee Min
Keyword(s):  
Graphene Oxide ◽  
Enzymatic Activity

Improved enzymatic activity by oriented immobilization on graphene oxide with tunable surface heterogeneity

2021 ◽  
Vol 216 ◽  
pp. 108788
Author(s):  
Wenfeng Zhou ◽  
Yuan Rao ◽  
Wei Zhuang ◽  
Lei Ge ◽  
Rijia Lin ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Enzymatic Activity ◽  
Surface Heterogeneity ◽  
Oriented Immobilization

The Effects of Graphene Oxide and Partially Reduced Graphene Oxide on the Enzymatic Activity of Microbial Transglutaminase in Gelatin

MRS Advances ◽  
2019 ◽  
Vol 4 (15) ◽  
pp. 879-887
Author(s):  
Rebecca Isseroff ◽  
Jerry Reyes ◽  
Roshan Reddy ◽  
Nicholas Williams ◽  
Miriam Rafailovich
Keyword(s):  
Graphene Oxide ◽  
Enzymatic Activity ◽  
Enzymatic Catalysis ◽  
Gelation Time ◽  
Crosslinking Density ◽  
Industrial Applications ◽  
Microbial Transglutaminase ◽  
Water Mixture ◽  
Gelatin Solution ◽  
Order Of Magnitude

ABSTRACTA significant drawback of enzyme use in industrial applications is its lack of stability. Graphene oxide (GO) has previously been investigated for enzyme immobilization and enhancement of enzymatic catalysis. Microbial transglutaminase (MTG) is an enzyme that is used to modify food proteins, increase durability of textiles, and crosslink hydrogels for drug delivery. We tested the effects of adding GO and partially reduced GO (pRGO) to water solutions of gelatin and then crosslinking it with MTG, measuring both the resulting gelatin modulus and then the time it took for the onset of gelation. We found that the presence of pRGO in a gelatin-MTG-water mixture (when using 0.75 g MTG in 10 ml of gelatin solution) significantly increases the modulus by 60% more than the control. Using this same concentration of MTG, we measured the onset of gelation time and found that pRGO in gelatin solution reduces the onset of gelation time by nearly 50% while inducing a very large increase in viscosity by three orders of magnitude, whereas the addition of GO increases the onset of gelation time by 33% and decreases the viscosity of the gel by more than one order of magnitude. The very large enhancement by pRGO of the viscosity may be due to pRGO’s electron withdrawing ability and/or may also be due to adsorption of gelatin to the pRGO platelets which effectively increases the crosslinking density through non-enzymatic processes assisting the enzymatic activity.

Photoswitching enzymatic activity of horseradish peroxidase by graphene oxide for colorimetric immunoassay

2019 ◽  
Vol 145 ◽  
pp. 111707 ◽  
Author(s):  
Genxia Cao ◽  
Dongxue Sun ◽  
Tiantian Gu ◽  
Yuming Dong ◽  
Guang-Li Wang
Keyword(s):  
Graphene Oxide ◽  
Horseradish Peroxidase ◽  
Enzymatic Activity ◽  
Colorimetric Immunoassay

Graphene oxide enhanced specificity at aptamer and its application to multiplexed enzymatic activity sensing

RSC Advances ◽  
2016 ◽  
Vol 6 (14) ◽  
pp. 11815-11821 ◽  
Author(s):  
Xiaojing Xing ◽  
Xueguo Liu ◽  
Ying Zhou ◽  
Dangdang Xu ◽  
Daiwen Pang ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Creatine Kinase ◽  
Graphene Oxide ◽  
Enzymatic Activity ◽  
Fluorescence Assay ◽  
Activity Sensing

Based on the finding that sufficient GO could enhance the specificity of an ATP aptamer, we proposed a fluorescence assay for alkaline phosphatase and creatine kinase.

scholarly journals Effects of partially reduced graphene oxide on the enzymatic activity of thrombin

2020 ◽  
Author(s):  
Rebecca Isseroff ◽  
Miriam Rafailovich ◽  
Yuval Shmueli ◽  
Juyi Li ◽  
Jonathan Lederer ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Enzymatic Activity ◽  
Reduced Graphene Oxide ◽  
Reduced Graphene ◽  
Partially Reduced Graphene Oxide

A graphene oxide-based fluorescence assay for the sensitive detection of DNA exonuclease enzymatic activity

The Analyst ◽  
2019 ◽  
Vol 144 (21) ◽  
pp. 6231-6239 ◽  
Author(s):  
Xiao Liu ◽  
Yingfen Wu ◽  
Xu Wu ◽  
Julia Xiaojun Zhao
Keyword(s):  
Graphene Oxide ◽  
Enzymatic Activity ◽  
Limit Of Detection ◽  
Fluorescent Sensor ◽  
Fluorescence Assay ◽  
Sensitive Detection ◽  
Exo Iii

A simple and ultra-sensitive fluorescent sensor was developed to detect the enzymatic activity of Exo III with a limit of detection of 0.001 U mL−1.

scholarly journals Effects of partially reduced graphene oxide on the enzymatic activity of thrombin

2020 ◽  
Author(s):  
Rebecca Isseroff ◽  
Miriam Rafailovich ◽  
Yuval Shmueli ◽  
Juyi Li ◽  
Jonathan Lederer ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Enzymatic Activity ◽  
Reduced Graphene Oxide ◽  
Reduced Graphene ◽  
Partially Reduced Graphene Oxide

scholarly journals Biodegradation of graphene materials catalyzed by human eosinophil peroxidase

2021 ◽  
Author(s):  
Rajendra Kurapati ◽  
Cristina Martìn ◽  
Vincenzo Palermo ◽  
Yuta Nishina ◽  
Alberto Bianco
Keyword(s):  
Graphene Oxide ◽  
Enzymatic Activity ◽  
Immune Cells ◽  
Human Eosinophil ◽  
Eosinophil Peroxidase ◽  
Human Immune ◽  
Different Sources

The enzymatic activity of eosinophil peroxidase secreted by human immune cells leads to degradation of different sources of graphene oxide.

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