Graphene oxide enhanced specificity at aptamer and its application to multiplexed enzymatic activity sensing

RSC Advances ◽  
2016 ◽  
Vol 6 (14) ◽  
pp. 11815-11821 ◽  
Author(s):  
Xiaojing Xing ◽  
Xueguo Liu ◽  
Ying Zhou ◽  
Dangdang Xu ◽  
Daiwen Pang ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Creatine Kinase ◽  
Graphene Oxide ◽  
Enzymatic Activity ◽  
Fluorescence Assay ◽  
Activity Sensing

Based on the finding that sufficient GO could enhance the specificity of an ATP aptamer, we proposed a fluorescence assay for alkaline phosphatase and creatine kinase.

A graphene oxide-based fluorescence assay for the sensitive detection of DNA exonuclease enzymatic activity

The Analyst ◽  
2019 ◽  
Vol 144 (21) ◽  
pp. 6231-6239 ◽  
Author(s):  
Xiao Liu ◽  
Yingfen Wu ◽  
Xu Wu ◽  
Julia Xiaojun Zhao
Keyword(s):  
Graphene Oxide ◽  
Enzymatic Activity ◽  
Limit Of Detection ◽  
Fluorescent Sensor ◽  
Fluorescence Assay ◽  
Sensitive Detection ◽  
Exo Iii

A simple and ultra-sensitive fluorescent sensor was developed to detect the enzymatic activity of Exo III with a limit of detection of 0.001 U mL−1.

Soil response to chemicals used in a field experiment

2013 ◽  
Vol 27 (2) ◽  
pp. 151-158 ◽  
Author(s):  
S. Jezierska-Tys ◽  
A. Rutkowska
Keyword(s):  
Alkaline Phosphatase ◽  
Enzymatic Activity ◽  
Field Experiment ◽  
Soil Microorganisms ◽  
Block Design ◽  
Soil Samples ◽  
Alkaline Phosphatases ◽  
Soil Response ◽  
Negative Effect ◽  
Set Up

Abstract The effect of chemicals (Reglone 200 SL and Elastiq 550 EC) on soil microorganisms and their enzymatic activity was estimated. The study was conducted in a field experiment which was set up in the split-block design and comprised three treatments. Soil samples were taken six times, twice in each year of study. The results showed that the application of chemicals generally had no negative effect on the number of soil microorganisms. The application of Reglone 200 SL caused an increase of proteolytic and ureolytic activity and affected the activity of dehydrogenases, acid and alkaline phosphatases in the soil. The soil subjected of Elastiq 550 EC was characterized by lower activity of dehydrogenases, protease, urease and alkaline phosphatase.

scholarly journals A retrospective cross-sectional study for predicting 72-h mortality in patients with serum aspartate aminotransferase levels ≥ 3000 U/L

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Kai Saito ◽  
Hitoshi Sugawara ◽  
Tamami Watanabe ◽  
Akira Ishii ◽  
Takahiko Fukuchi
Keyword(s):  
Risk Factors ◽  
Alkaline Phosphatase ◽  
Creatine Kinase ◽  
Cross Sectional Study ◽  
Sectional Study ◽  
Cross Sectional ◽  
Sodium Potassium ◽  
Factors Associated ◽  
C Statistic ◽  
Serum Aspartate Aminotransferase

AbstractRisk factors associated with 72-h mortality in patients with extremely high serum aspartate aminotransferase levels (AST; ≥ 3000 U/L) are unknown. This single-centre, retrospective, case-controlled, cross-sectional study obtained data from medical records of adult patients treated at Saitama Medical Center, Japan, from 2005 to 2019. We conducted a multivariate logistic after adjusting for age, sex, height, weight, body mass index, Brinkman Index, vital signs, biochemical values, updated Charlson Comorbidity Index (CCI) score, CCI components, and underlying causes. A logistic regression model with selected validity risks and higher C-statistic for predicting 72-h mortality was established. During the 15-year period, 428 patients (133 non-survivors and 295 survivors [cases and controls by survival < 72 and ≥ 72 h, respectively]) with AST levels ≥ 3000 U/L were identified. The 72-h mortality rate was 133/428 (31.1%). The model used for predicting 72-h mortality through the assessment of alkaline phosphatase, creatine kinase, serum sodium, potassium, and phosphorus levels had a C-statistic value of 0.852 (sensitivity and specificity, 76.6%). The main independent risk factors associated with 72-h mortality among patients with AST levels ≥ 3000 U/L included higher serum values of alkaline phosphatase, creatine kinase, serum sodium, potassium, and phosphorus.

Improved enzymatic activity by oriented immobilization on graphene oxide with tunable surface heterogeneity

2021 ◽  
Vol 216 ◽  
pp. 108788
Author(s):  
Wenfeng Zhou ◽  
Yuan Rao ◽  
Wei Zhuang ◽  
Lei Ge ◽  
Rijia Lin ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Enzymatic Activity ◽  
Surface Heterogeneity ◽  
Oriented Immobilization

scholarly journals Replacing Maize Grain with Ancient Wheat Lines By-Products in Organic Laying Hens’ Diet Affects Intestinal Morphology and Enzymatic Activity

2021 ◽  
Vol 13 (12) ◽  
pp. 6554
Author(s):  
Nicola Francesco Addeo ◽  
Basilio Randazzo ◽  
Ike Olivotto ◽  
Maria Messina ◽  
Francesca Tulli ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Enzymatic Activity ◽  
Laying Hens ◽  
Triticum Aestivum L ◽  
Treated Group ◽  
Intestinal Morphology ◽  
Control Group ◽  
Positive Effects ◽  
Maize Grain ◽  
By Products

The effects of replacement of maize grain with ancient wheat by-products on intestinal morphometry and enzymatic activity in laying hens was studied. Eighty hens were divided into two groups (40 each, 8 replicates, 5 hens/replicate) fed two isoproteic and isoenergetic diets. In the treated group, part of the maize was replaced by a mix of ancient grains (AGs) middling, in a 50:50 ratio of Triticum aestivum L. var. spelta (spelt) and Triticum durum dicoccum L. (emmer wheat). The AG diet affected the weight of all the large intestine tracts, decreasing the weight of caeca (p < 0.01) and increasing those of colon (p < 0.01), rectum and cloaca (p < 0.05). Villus height in the AG group was higher (p < 0.01) than the control for the duodenum and jejunum, while for the ileum, the control group showed the highest values (p < 0.01). The submucosa thickness was higher (p < 0.01) in the control group for the duodenum and ileum, while the jejunum for the AG group showed the highest (p < 0.05) submucosa thickness. The crypts depth was higher (p < 0.01) in the control group for the duodenum and ileum. Enzyme activity was enhanced by AGs (p < 0.01) in the duodenum. Regarding the jejunum, sucrase-isomaltase and alkaline phosphatase had higher activity (p < 0.05 and p < 0.01, respectively) in the AG group. In the ileum, sucrase-isomaltase showed higher activity (p < 0.01) in the control group, while alkaline phosphatase showed the highest values (p < 0.05) in the AG group. Overall, results suggested that the dietary inclusion of AGs exerted positive effects in hens, showing an improved intestinal function.

scholarly journals Necessity of Enzymatic Activity of Alkaline Phosphatase for Mineralization of Osteoblastic Cells

2002 ◽  
Vol 88 (3) ◽  
pp. 262-269 ◽  
Author(s):  
Yuki Sugawara ◽  
Kuniaki Suzuki ◽  
Mino Koshikawa ◽  
Masaki Ando ◽  
Junichiro Iida
Keyword(s):  
Alkaline Phosphatase ◽  
Enzymatic Activity ◽  
Osteoblastic Cells

Carbon Quantum Dots-Based Recyclable Real-Time Fluorescence Assay for Alkaline Phosphatase with Adenosine Triphosphate as Substrate

2015 ◽  
Vol 87 (5) ◽  
pp. 2966-2973 ◽  
Author(s):  
Zhaosheng Qian ◽  
Lujing Chai ◽  
Cong Tang ◽  
Yuanyuan Huang ◽  
Jianrong Chen ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Alkaline Phosphatase ◽  
Real Time ◽  
Adenosine Triphosphate ◽  
Carbon Quantum Dots ◽  
Fluorescence Assay

scholarly journals The effect of different copper doses and organic fertilisation on soil’s enzymatic activity

2020 ◽  
Vol 66 (No. 2) ◽  
pp. 93-98
Author(s):  
Beata Kuziemska ◽  
Andrzej Wysokiński ◽  
Joanna Trębicka
Keyword(s):  
Alkaline Phosphatase ◽  
Acid Phosphatase ◽  
Enzymatic Activity ◽  
Phosphatase Activity ◽  
Acid Phosphatase Activity ◽  
Organic Materials ◽  
Chicken Manure ◽  
Test Plant ◽  
Orchard Grass ◽  
Organic Fertilisation

A three-year pot experiment carried out in the vegetation hall in 2014–2016 included studying the enzymatic activity of soil, into which various amounts of copper: (100, 200 and 300 mg Cu/kg soil) and organic materials (cattle manure, chicken manure, post-mushroom substrate) were introduced, used separately, at a soil-introduction dose of 2 g C<sub>org</sub>/kg. Copper and organic materials were used once, only in the first year of the study, before sowing test plant orchard grass. In soil collected after the last (fourth) swath of grass in each year of the study, the activity of urease, dehydrogenases, acid, and alkaline phosphatase was determined. Applications of copper to the soil, regardless of its dose, resulted in a decrease in urease, dehydrogenases and alkaline phosphatase and an increase in acid phosphatase activity. The inactivating effect of this metal on the activity of urease, dehydrogenases and alkaline phosphatase increased with the increase of its dose. Organic fertilisation generally increased the enzymatic activity of the analysed soil. In subsequent years of the study, urease and alkaline phosphatase activity decreased, while acid phosphatase activity increased. Dehydrogenase activity did not change significantly in subsequent years of the study.  

A fluorescence assay for microRNA let-7a by a double-stranded DNA modified gold nanoparticle nanoprobe combined with graphene oxide

The Analyst ◽  
2020 ◽  
Vol 145 (4) ◽  
pp. 1190-1194 ◽  
Author(s):  
Yuanyuan Gao ◽  
Jingjing Tian ◽  
Xing Zhang ◽  
Bin Qiao ◽  
Yang Cao ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Gold Nanoparticle ◽  
Fluorescence Assay ◽  
Double Stranded Dna ◽  
Fluorescence Switching

A fluorescence switching platform was developed to monitor target microRNA let-7a by coupling dsDNA–AuNPs with the GO nanosheet.

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