Efficient enrichment of glycopeptides using phenylboronic acid polymer brush modified silica microspheres

2014 ◽  
Vol 2 (16) ◽  
pp. 2276-2281 ◽  
Author(s):  
Xiuling Li ◽  
Hongliang Liu ◽  
Guangyan Qing ◽  
Shutao Wang ◽  
Xinmiao Liang
Keyword(s):  
Synergistic Effect ◽  
High Selectivity ◽  
Phenylboronic Acid ◽  
Polymer Brush ◽  
Hydrogen Binding ◽  
Silica Microspheres ◽  
Modified Silica ◽  
Hydrophilic Nature

Phenylboronic acid (PBA) polymer brush modified silica demonstrated high selectivity for glycopeptides attributed to the synergistic effect of polyvalent interactions, hydrogen binding and hydrophilic nature provided by the polymer brush.

Highly Selectivity Molecularly Imprinted Fluorescence Sensor Based on Carbon Quantum Dots for the Determination of Anthraquinones

2021 ◽  
Vol 21 (4) ◽  
pp. 2109-2116
Author(s):  
Yangyang Li ◽  
Xiuzhen Qiu ◽  
Yulin Wang ◽  
Huishi Guo ◽  
Libo Nie
Keyword(s):  
Quantum Dots ◽  
High Selectivity ◽  
Theoretical Calculations ◽  
Carbon Quantum Dots ◽  
Fluorescence Sensor ◽  
Template Molecule ◽  
Functional Monomer ◽  
Hydrogen Binding ◽  
Molecularly Imprinted ◽  
Target Molecules

Due to the complexity of traditional Chinese medicines (TCMs), it is very important to develop a method that can recognize anthraquinones, the active ingredients in TCMs, with high selectivity. Here, a molecularly imprinted fluorescence sensor was coated on the surface of carbon quantum dots (CDs). Allobarbital was used as functional monomer for this application using theoretical calculations and was successfully synthesized and characterized. The template molecule chrysophanol was combined with the functional monomer allobarbital using a hydrogen bond array. Then, a series of adsorption experiments were performed to study the specific recognition of anthraquinones by the prepared sensors. The results showed that the prepared sensor had a good linear response to concentrations of chrysophanol in the concentration range 0.5 mg · L-1 to 8.0 mg · L-1, a low detection limit (5.0 μg · L-1), high stability, and a short response time (20 min). Additionally, the obtained fluorescence sensor was successfully applied to selectively recognize anthraquinones in TCMs with recoveries of 90.1% to 101.7%. The prepared sensor displays excellent sensitivity and high selectivity towards anthraquinones, mainly due to the specific hydrogen binding sites for the target molecules. Overall, this fluorescence sensor can selectively recognize anthraquinones in TCMs, and provide a method for quality monitoring and rational utilization of TCMs.

scholarly journals Dual-Emission Fluorescent Microspheres for the Detection of Biothiols and Hg2+

Materials ◽  
2018 ◽  
Vol 11 (11) ◽  
pp. 2232 ◽  
Author(s):  
Jiahui Wang ◽  
Hao Zhang ◽  
Ruifang Guan
Keyword(s):  
Amino Acids ◽  
Detection Limit ◽  
Raw Material ◽  
Excitation Wavelength ◽  
Ratiometric Fluorescence ◽  
Silica Microspheres ◽  
Modified Silica ◽  
Fluorescent Microspheres ◽  
Dual Emission ◽  
Recognition Sites

Dual-emission nanosensor for Hg2+ detection was prepared by coupling CA-AEAPMS on the surface of RBS-doped modified silica microspheres. The CA-AEAPMS was synthesized by using N-(β-aminoethyl)-γ-aminopropyl methyldimethoxysilane (AEAPMS) and citric acid as the main raw material. The obtained nanosensor showed characteristic fluorescence emissions of Rhodamine B (red) and CA-AEAPMS (blue) under a single excitation wavelength (360 nm). Upon binding to Hg2+, only the fluorescence of CA-AEAPMS was quenched, resulting in the ratiometric fluorescence response of the dual-emission silica microspheres. This ratiometric nanosensor exhibited good selectivity to Hg2+ over other metal ions, because of the amide groups on the surface of CA-AEAPMS serving as the Hg2+ recognition sites. The ratio of F450/F580 linearly decreased with the increasing of Hg2+ concentration in the range of 0 to 3 × 10−6 M, and a detection limit was as low as 97 nM was achieved. Then, the addition of three thiol-containing amino acids (Cys, Hcy, GSH) to the quenched fluorescence solution with Hg2+ can restore the fluorescence, and the detection limits of the three biothiols (Cys, Hcy, GSH) are 0.133 μM, 0.086 μM, and 0.123 μM, respectively.

Selective capture and non-invasive release of cells using a thermoresponsive polymer brush with affinity peptides

2021 ◽  
Author(s):  
Kenichi Nagase ◽  
Masaki Shimura ◽  
Ruka Shimane ◽  
Kengo Hanaya ◽  
Sota Yamada ◽  
...  
Keyword(s):  
Block Copolymer ◽  
High Selectivity ◽  
Click Reaction ◽  
Polymer Brush ◽  
Thermoresponsive Polymer ◽  
Non Invasive ◽  
Cell Affinity ◽  
Copolymer Brush

Thermoresponsive block copolymer brush with cell affinity peptides was prepared via two steps of ATRP and subsequent click reaction. The prepared polymer brush can purify cells with high selectivity by simply changing temperature.

Synergistic Effect of Polyaniline Modified Silica Gel for Highly Efficient Separation of Non Resolvable Amino Acids

2013 ◽  
Vol 63 (5) ◽  
pp. 277-281 ◽  
Author(s):  
Asma Siddiq ◽  
Mohd Omaish Ansari ◽  
Ali Mohammad ◽  
Faiz Mohammad ◽  
Gaber E. El-Desoky
Keyword(s):  
Amino Acids ◽  
Synergistic Effect ◽  
Silica Gel ◽  
Modified Silica ◽  
Modified Silica Gel ◽  
Efficient Separation ◽  
Highly Efficient

Dual-Responsive Surfaces Modified with Phenylboronic Acid-Containing Polymer Brush To Reversibly Capture and Release Cancer Cells

2013 ◽  
Vol 135 (20) ◽  
pp. 7603-7609 ◽  
Author(s):  
Hongliang Liu ◽  
Yingying Li ◽  
Kang Sun ◽  
Junbing Fan ◽  
Pengchao Zhang ◽  
...  
Keyword(s):  
Cancer Cells ◽  
Phenylboronic Acid ◽  
Polymer Brush ◽  
Dual Responsive ◽  
Responsive Surfaces ◽  
Capture And Release
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