scholarly journals Dual-Emission Fluorescent Microspheres for the Detection of Biothiols and Hg2+

Materials ◽  
2018 ◽  
Vol 11 (11) ◽  
pp. 2232 ◽  
Author(s):  
Jiahui Wang ◽  
Hao Zhang ◽  
Ruifang Guan
Keyword(s):  
Amino Acids ◽  
Detection Limit ◽  
Raw Material ◽  
Excitation Wavelength ◽  
Ratiometric Fluorescence ◽  
Silica Microspheres ◽  
Modified Silica ◽  
Fluorescent Microspheres ◽  
Dual Emission ◽  
Recognition Sites

Dual-emission nanosensor for Hg2+ detection was prepared by coupling CA-AEAPMS on the surface of RBS-doped modified silica microspheres. The CA-AEAPMS was synthesized by using N-(β-aminoethyl)-γ-aminopropyl methyldimethoxysilane (AEAPMS) and citric acid as the main raw material. The obtained nanosensor showed characteristic fluorescence emissions of Rhodamine B (red) and CA-AEAPMS (blue) under a single excitation wavelength (360 nm). Upon binding to Hg2+, only the fluorescence of CA-AEAPMS was quenched, resulting in the ratiometric fluorescence response of the dual-emission silica microspheres. This ratiometric nanosensor exhibited good selectivity to Hg2+ over other metal ions, because of the amide groups on the surface of CA-AEAPMS serving as the Hg2+ recognition sites. The ratio of F450/F580 linearly decreased with the increasing of Hg2+ concentration in the range of 0 to 3 × 10−6 M, and a detection limit was as low as 97 nM was achieved. Then, the addition of three thiol-containing amino acids (Cys, Hcy, GSH) to the quenched fluorescence solution with Hg2+ can restore the fluorescence, and the detection limits of the three biothiols (Cys, Hcy, GSH) are 0.133 μM, 0.086 μM, and 0.123 μM, respectively.

scholarly journals Noval Dual-Emission Fluorescence Carbon Dots as a Ratiometric Probe for Cu2+ and ClO− Detection

Nanomaterials ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 1232
Author(s):  
Jiaqing Guo ◽  
Aikun Liu ◽  
Yutian Zeng ◽  
Haojie Cai ◽  
Shuai Ye ◽  
...  
Keyword(s):  
Carbon Dots ◽  
Background Noise ◽  
Good Recovery ◽  
Ratiometric Fluorescence ◽  
Dual Emission ◽  
Simple Method ◽  
Real Samples ◽  
Nitrogen Doped ◽  
Probe Concentration ◽  
Ratiometric Probe

The use of carbon dots (CDs) with dual emission based on ratiometric fluorescence has been attracting attention in recent times for more accurate ion detection since they help avoid interference from background noise, probe concentration, and complexity. Herein, novel dual-emission nitrogen-doped CDs (NCDs) were prepared by a simple method for Cu2+ and ClO- detection. The NCDs showed excellent anti-interference ability and selectivity for different emissions. In addition, a good linear relationship was observed between the fluorescence intensity (FI) of the NCD solutions in different emissions with Cu2+ (0–90 μM) and ClO- (0–75 μM). The limits of both Cu2+ detection and ClO- were very low, at 17.7 and 11.6 nM, respectively. The NCDs developed herein also showed a good recovery rate in water for Cu2+ and ClO− detection. Hence, they are expected to have a more extensive application prospect in real samples.

Fabrication of an “ion-imprinting” dual-emission quantum dot nanohybrid for selective fluorescence turn-on and ratiometric detection of cadmium ions

The Analyst ◽  
2016 ◽  
Vol 141 (20) ◽  
pp. 5886-5892 ◽  
Author(s):  
Jing Wang ◽  
Chenxing Jiang ◽  
Xiaoqi Wang ◽  
Ligeng Wang ◽  
Aimin Chen ◽  
...  
Keyword(s):  
Quantum Dot ◽  
Fluorescence Detection ◽  
Ratiometric Fluorescence ◽  
Dual Emission ◽  
Cadmium Ions ◽  
Turn On ◽  
Ratiometric Detection ◽  
Ion Imprinting ◽  
Fluorescence Turn On

A new CdTe@SiO2@CdSe nanohybrid was demonstrated for the ratiometric fluorescence detection of Cd2+via the turn-on model and “ion-imprinting” technique.

Dual-emission MOF⊃dye sensor for ratiometric fluorescence recognition of RDX and detection of a broad class of nitro-compounds

2018 ◽  
Vol 6 (19) ◽  
pp. 9183-9191 ◽  
Author(s):  
Hong-Ru Fu ◽  
Li-Bing Yan ◽  
Nai-Teng Wu ◽  
Lu-Fang Ma ◽  
Shuang-Quan Zang
Keyword(s):  
Ion Exchange ◽  
Broad Class ◽  
Nitro Compounds ◽  
Ratiometric Fluorescence ◽  
Dual Emission ◽  
Nitroaromatic Explosives ◽  
Nitro Explosives

A dual-emitting MOF-based sensor 1⊃HPTS was prepared through encapsulating the dye HPTS via an ion-exchange approach. 1⊃HPTS exhibits a broad response to nitro compounds including nitroaromatic explosives, aliphatic nitro-explosives and nitro-containing antibiotics.

scholarly journals Separation and detection limit of chiral amino acids in multiple components by analytical techniques

2004 ◽  
Vol 53 (12) ◽  
pp. 1507-1514
Author(s):  
Yoshinori TAKANO ◽  
Katsumi MARUMO ◽  
Kensei KOBAYASHI ◽  
Jun-ichi TAKAHASHI
Keyword(s):  
Amino Acids ◽  
Detection Limit ◽  
Analytical Techniques ◽  
Multiple Components ◽  
Chiral Amino Acids

scholarly journals AMINO ACID COMPOSITION OF LEAVES OF THREE SPECIES OF ARTEMISIA L. GROWING IN THE ELTON REGION

2021 ◽  
pp. 219-225
Author(s):  
Галина Табаленкова
Keyword(s):  
Amino Acids ◽  
Czech Republic ◽  
Amino Acid ◽  
Dry Weight ◽  
Biologically Active ◽  
Raw Material ◽  
Buffer System ◽  
Amino Acid Analyzer ◽  
Growing Conditions ◽  
High Level

The results of studies of the qualitative composition and quantitative content of amino acids (AAs) in the leaves of three plant species of the genus Artemisia, widespread in the Elton region, were presented. Protein AAs were determined on an AAA T-339 amino acid analyzer (Czech Republic) after hydrolysis of a sample in 6N HCl at 105 °C for 24 h, free AAs – on an AAA-400 amino acid analyzer (Czech Republic) in a lithium buffer system. The protein AAs amount varied from 66 mg / g in A. lerchiana to 113 mg / g dry weight in A. santonica. 17 AAs were found in composition of these species, aspartic and glutamic acids were dominant. The content of free AAs varied from 4.4 mg / g in A. santonica to 8.3 mg / g dry weight in A. pauciflora. 14 AAs have been identified, among them proline was the predominant free AA. The share of proline was 75-81% of the total free AAs. Among the minor components, 3-4 compounds with a content above 2% dominated. The free AAs contain 3 non-proteinogenic ones (ornithine, citruline, and γ-aminobutyric acid). A. lerchiana and A. pauciflora species were similar in protein and free amino acids, probably due to the same growing conditions. A high level of free proline, together with a complex of biologically active substances in Artemisia species, which grow abundantly in the Elton region, allow to consider the possibility of their use as a medicinal raw material.

scholarly journals An Identification and Study of Amino Acids of Erigeron annuus Herb

2021 ◽  
Vol 16 (2) ◽  
pp. 88-92
Author(s):  
S. Kovalev ◽  
A. Golovach ◽  
V. Kovalev
Keyword(s):  
Amino Acids ◽  
Liquid Chromatography ◽  
High Performance ◽  
Total Content ◽  
Amino Acid Profile ◽  
Reversed Phase ◽  
Raw Material ◽  
Precolumn Derivatization ◽  
Amino Acids Composition ◽  
Erigeron Annuus

Amino acids in the extract of Erigeron annuus herb were determined using an automatic precolumn derivatization with fluorenylmethyl-chloroformate and reversed-phase liquid chromatography with fluorescence and UV Vis detection. This objective is reached with automatic derivatization using o-phthalaldehyde (OPA) for primary amino acids and 9-Fluorenylmethyl chloroformate (FMOC) for secondary amino acids. Then derivatization integrates into high performance liquid chromatography (HPLC). The applied procedure is fast with easily reproduced results. The insufficient knowledge about amino acids composition of herb of Erigeron annuus is the basis for study. This work reports on content of 16 free and bound amino acids (391.41 μg/mg) in the plant raw material and influence’s evaluation of different extraction types on the amino acid profile. The total content of free amino acids was 4.66 μg/mg; proline prevailed (2.498 μg/mg). The total content of bound amino acids was 386.7 μg/mg; proline (146.8 μg/mg), arginine (67.8 μg/mg), phenylalanine (25.8 μg/mg), asparagine (24.3 μg/mg), histidine (20.4 μg/mg), alanine (18.2 μg/mg), serine (16.6 μg/mg), valine (16.0 μg/mg) were the dominant amino acids. Nine amino acids were classified as essential.

scholarly journals A dual emission metal–organic framework for rapid ratiometric fluorescence detection of CO32− in seawater

RSC Advances ◽  
2020 ◽  
Vol 10 (42) ◽  
pp. 24764-24771
Author(s):  
Yu Wei ◽  
Yan Xia
Keyword(s):  
Fluorescence Detection ◽  
Fluorescent Sensor ◽  
Metal Organic Framework ◽  
Ratiometric Fluorescence ◽  
Dual Emission ◽  
Metal Organic ◽  
Organic Framework

A dual emission metal–organic framework (IRMOF-10-Eu) was prepared and used as a ratiometric fluorescent sensor for CO32− detection.

scholarly journals A 7-Hydroxybenzoxazinone-Containing Fluorescence Turn-On Probe for Biothiols and Its Bioimaging Applications

Molecules ◽  
2019 ◽  
Vol 24 (17) ◽  
pp. 3102 ◽  
Author(s):  
Bin Li ◽  
Datong Zhang ◽  
Ruibing An ◽  
Yaling Zhu
Keyword(s):  
Amino Acids ◽  
Detection Limit ◽  
High Resistance ◽  
Hela Cells ◽  
Fluorescence Enhancement ◽  
Stokes Shift ◽  
Potential Utility ◽  
Strong Fluorescence ◽  
Turn On ◽  
Fluorescence Turn On

In this work, a novel 7-hydroxybenzoxazinone-based fluorescent probe (PBD) for the selective sensing of biothiols is reported. Upon treatment with biothiols, PBD shows a strong fluorescence enhancement (up to 70-fold) and a large Stokes shift (155 nm). Meanwhile, this probe exhibits high resistance to interference from other amino acids and competing species. PBD features good linearity ranges with a low detection limit of 14.5 nM for glutathione (GSH), 17.5 nM for cysteine (Cys), and 80.0 nM for homocysteine (Hcy), respectively. Finally, the potential utility of this probe for biothiol sensing in living HeLa cells is demonstrated.

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