N-Terminal Aliphatic Residues Dictate the Structure, Stability, Assembly, and Small Molecule Binding of the Coiled-Coil Region of Cartilage Oligomeric Matrix Protein

Biochemistry ◽  
2009 ◽  
Vol 48 (36) ◽  
pp. 8559-8567 ◽  
Author(s):  
Susheel K. Gunasekar ◽  
Mukta Asnani ◽  
Chandani Limbad ◽  
Jennifer S. Haghpanah ◽  
Wendy Hom ◽  
...  
Keyword(s):  
Small Molecule ◽  
Cartilage Oligomeric Matrix Protein ◽  
Matrix Protein ◽  
Coiled Coil ◽  
Structure Stability ◽  
Coiled Coil Region

Polypeptide-engineered physical hydrogels designed from the coiled-coil region of cartilage oligomeric matrix protein for three-dimensional cell culture

2014 ◽  
Vol 2 (20) ◽  
pp. 3123-3132 ◽  
Author(s):  
Ming-Hao Yao ◽  
Jie Yang ◽  
Ming-Shuo Du ◽  
Ji-Tao Song ◽  
Yong Yu ◽  
...  
Keyword(s):  
Cell Culture ◽  
Self Assembly ◽  
Cartilage Oligomeric Matrix Protein ◽  
Matrix Protein ◽  
Three Dimensional ◽  
Coiled Coil ◽  
The Self ◽  
Coiled Coil Region ◽  
Dimensional Cell

A class of physical hydrogels photo-cross-linked from multi-branched photopolymeriized monomers based on the self-assembly of coiled-coil polypeptide P is developed.

scholarly journals All-trans retinol, vitamin D and other hydrophobic compounds bind in the axial pore of the five-stranded coiled-coil domain of cartilage oligomeric matrix protein

1998 ◽  
Vol 17 (18) ◽  
pp. 5265-5272 ◽  
Author(s):  
Yuan Guo ◽  
Damir Bozic ◽  
Vladimir N. Malashkevich ◽  
Richard A. Kammerer ◽  
Therese Schulthess ◽  
...  
Keyword(s):  
Vitamin D ◽  
Cartilage Oligomeric Matrix Protein ◽  
Matrix Protein ◽  
Coiled Coil ◽  
Hydrophobic Compounds ◽  
Coiled Coil Domain

Can Tocilizumab Decrease Cartilage Oligomeric Matrix Protein Levels and Disease Activity in Patients with Long-Standing Rheumatoid Arthritis?

2015 ◽  
Vol 11 (999) ◽  
pp. 1-1
Author(s):  
Maurizio Benucci ◽  
Francesca Meacci ◽  
Mariangela Manfredi ◽  
Francesca Gobbi ◽  
Maria Infantino ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Disease Activity ◽  
Cartilage Oligomeric Matrix Protein ◽  
Matrix Protein ◽  
Protein Levels

scholarly journals Analysis of Cartilage Oligomeric Matrix Protein (COMP) in Synovial Fluid, Serum and Urine from 51 Racehorses with Carpal Bone Fracture

2008 ◽  
Vol 70 (9) ◽  
pp. 915-921 ◽  
Author(s):  
Koh ARAI ◽  
Masaaki TAGAMI ◽  
Takashi HATAZOE ◽  
Eikoh NISHIMATSU ◽  
Yuri SHIMIZU ◽  
...  
Keyword(s):  
Synovial Fluid ◽  
Bone Fracture ◽  
Cartilage Oligomeric Matrix Protein ◽  
Matrix Protein ◽  
Carpal Bone ◽  
Serum And Urine

scholarly journals A Coiled-Coil Domain of Melanophilin Is Essential for Myosin Va Recruitment and Melanosome Transport in Melanocytes

2006 ◽  
Vol 17 (11) ◽  
pp. 4720-4735 ◽  
Author(s):  
Alistair N. Hume ◽  
Abul K. Tarafder ◽  
José S. Ramalho ◽  
Elena V. Sviderskaya ◽  
Miguel C. Seabra
Keyword(s):  
Coiled Coil ◽  
Binding Domain ◽  
Actin Binding ◽  
Binding Studies ◽  
Null Cell ◽  
Coiled Coil Region ◽  
Actin Binding Domain ◽  
Transport Assay ◽  
Myosin Va

Melanophilin (Mlph) regulates retention of melanosomes at the peripheral actin cytoskeleton of melanocytes, a process essential for normal mammalian pigmentation. Mlph is proposed to be a modular protein binding the melanosome-associated protein Rab27a, Myosin Va (MyoVa), actin, and microtubule end-binding protein (EB1), via distinct N-terminal Rab27a-binding domain (R27BD), medial MyoVa-binding domain (MBD), and C-terminal actin-binding domain (ABD), respectively. We developed a novel melanosome transport assay using a Mlph-null cell line to study formation of the active Rab27a:Mlph:MyoVa complex. Recruitment of MyoVa to melanosomes correlated with rescue of melanosome transport and required intact R27BD together with MBD exon F–binding region (EFBD) and unexpectedly a potential coiled-coil forming sequence within ABD. In vitro binding studies indicate that the coiled-coil region enhances binding of MyoVa by Mlph MBD. Other regions of Mlph reported to interact with MyoVa globular tail, actin, or EB1 are not essential for melanosome transport rescue. The strict correlation between melanosomal MyoVa recruitment and rescue of melanosome distribution suggests that stable interaction with Mlph and MyoVa activation are nondissociable events. Our results highlight the importance of the coiled-coil region together with R27BD and EFBD regions of Mlph in the formation of the active melanosomal Rab27a-Mlph-MyoVa complex.

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