Plasmonically active nanorods for delivery of bio-active agents and high-sensitivity SERS detection in planta

RSC Advances ◽  
2014 ◽  
Vol 4 (110) ◽  
pp. 64985-64993 ◽  
Author(s):  
Zeid A. Nima ◽  
Mohamed H. Lahiani ◽  
Fumiya Watanabe ◽  
Yang Xu ◽  
Mariya V. Khodakovskaya ◽  
...  
Keyword(s):  
High Sensitivity ◽  
In Planta ◽  
Sers Detection

Advanced Design of Microfluidic Chip Based on SPP-LSP Plasmonic Coupling for SERS Detection with High Sensitivity and Reliability

2019 ◽  
Vol 123 (50) ◽  
pp. 30492-30498 ◽  
Author(s):  
V. Burtsev ◽  
E. Miliutina ◽  
M. Erzina ◽  
Y. Kalachyova ◽  
R. Elashnikov ◽  
...  
Keyword(s):  
Microfluidic Chip ◽  
High Sensitivity ◽  
Plasmonic Coupling ◽  
Sers Detection

Quantitative SERS Detection of Trace Glutathione with Internal Reference Embedded Au-core/Ag-shell Nanoparticles

Nano LIFE ◽  
2016 ◽  
Vol 06 (03n04) ◽  
pp. 1642003 ◽  
Author(s):  
Yan Zhou ◽  
Rui Ding
Keyword(s):  
Reference Method ◽  
High Sensitivity ◽  
Internal Reference ◽  
Shell Nanoparticles ◽  
New Strategy ◽  
Surface Enhanced Raman ◽  
Sers Detection ◽  
Quantitative Sers ◽  
Shell Particles ◽  
Improved Accuracy

Surface-enhanced Raman scattering (SERS) has been widely studied and applied for over three decades. However, reliable SERS detection of molecules with low polarizability is still suffering from poor sensitivity and reproducibility. In this paper, we have reported a new strategy for performing quantitative SERS detection of Raman insensitive Glutathione (GSH), based on GSH-induced replacement of a highly Raman sensitive four-mercaptopyridine (MP) adsorbed on the surface of four-aminothiophenol (ATP) embedded Au-core/Ag-shell particles. This replacement led to a strong decrease of the MP SERS signal, which was used to determine the concentration of GSH. The adoption of GSH-induced Raman probe replacement leads to high sensitivity, while the use of internal reference method provides an improved accuracy of the GSH quantification.

scholarly journals High Sensitivity, High Selectivity SERS Detection of MnSOD Using Optical Nanoantennas Functionalized with Aptamers

2015 ◽  
Vol 119 (27) ◽  
pp. 15532-15540 ◽  
Author(s):  
Maximilien Cottat ◽  
Cristiano D’Andrea ◽  
Ryohei Yasukuni ◽  
Natalia Malashikhina ◽  
Ruta Grinyte ◽  
...  
Keyword(s):  
High Selectivity ◽  
High Sensitivity ◽  
Sers Detection ◽  
Optical Nanoantennas

A porous Au–Ag hybrid nanoparticle array with broadband absorption and high-density hotspots for stable SERS analysis

Nanoscale ◽  
2019 ◽  
Vol 11 (19) ◽  
pp. 9587-9592 ◽  
Author(s):  
Kuanguo Li ◽  
Guangju Liu ◽  
Sheng Zhang ◽  
Yanqiu Dai ◽  
Sonia Ghafoor ◽  
...  
Keyword(s):  
Low Cost ◽  
High Sensitivity ◽  
High Density ◽  
Nanoparticle Array ◽  
Broadband Absorption ◽  
Sers Detection ◽  
Hybrid Nanoparticle

An Au–Ag hybrid nanoparticle array with dense hotspots was constructed through a low-cost route for SERS detection with high sensitivity and stability.

Multiplexed electrochemical and SERS dual-mode detection of stroke biomarkers: rapid screening with high sensitivity

2019 ◽  
Vol 43 (34) ◽  
pp. 13381-13387 ◽  
Author(s):  
Jingyi Sun ◽  
Yi Zhao ◽  
Yajun Hou ◽  
Hanxia Li ◽  
Mingfeng Yang ◽  
...  
Keyword(s):  
Neuron Specific Enolase ◽  
High Sensitivity ◽  
Rapid Screening ◽  
Label Free ◽  
Raman Spectroscopic ◽  
Mode Detection ◽  
Β Protein ◽  
Highly Sensitive ◽  
Surface Enhanced Raman ◽  
Sers Detection

In this work, a real-time assay for a highly sensitive, label-free, multiplexed electrochemical and surface-enhanced Raman spectroscopic (SERS) detection of stroke biomarkers by neuron-specific enolase (NSE) and S100-β protein was developed using lateral flow devices.

scholarly journals H2O2-triggered Retrograde Signaling from Chloroplasts to Nucleus Plays Specific Role in Response to Stress

2012 ◽  
Vol 287 (15) ◽  
pp. 11717-11729 ◽  
Author(s):  
Takanori Maruta ◽  
Masahiro Noshi ◽  
Aoi Tanouchi ◽  
Masahiro Tamoi ◽  
Yukinori Yabuta ◽  
...  
Keyword(s):  
High Sensitivity ◽  
Retrograde Signaling ◽  
Plant Responses ◽  
Large Set ◽  
In Planta ◽  
Binding Factor ◽  
Signaling Function ◽  
Negative Effect ◽  
Response To Stress ◽  
Membrane Bound

Recent findings have suggested that reactive oxygen species (ROS) are important signaling molecules for regulating plant responses to abiotic and biotic stress and that there exist source- and kind-specific pathways for ROS signaling. In plant cells, a major source of ROS is chloroplasts, in which thylakoid membrane-bound ascorbate peroxidase (tAPX) plays a role in the regulation of H2O2 levels. Here, to clarify the signaling function of H2O2 derived from the chloroplast, we created a conditional system for producing H2O2 in the organelle by chemical-dependent tAPX silencing using estrogen-inducible RNAi. When the expression of tAPX was silenced in leaves, levels of oxidized protein in chloroplasts increased in the absence of stress. Microarray analysis revealed that tAPX silencing affects the expression of a large set of genes, some of which are involved in the response to chilling and pathogens. In response to tAPX silencing, the transcript levels of C-repeat/DRE binding factor (CBF1), a central regulator for cold acclimation, was suppressed, resulting in a high sensitivity of tAPX-silenced plants to cold. Furthermore, tAPX silencing enhanced the levels of salicylic acid (SA) and the response to SA. Interestingly, we found that tAPX silencing-responsive genes were up- or down-regulated by high light (HL) and that tAPX silencing had a negative effect on expression of ROS-responsive genes under HL, suggesting synergistic and antagonistic roles of chloroplastic H2O2 in HL response. These findings provide a new insight into the role of H2O2-triggered retrograde signaling from chloroplasts in the response to stress in planta.

scholarly journals Sensitivity to Azoxystrobin Among Isolates of Uncinula necator: Baseline Distribution and Relationship to Myclobutanil Sensitivity

Plant Disease ◽  
2002 ◽  
Vol 86 (4) ◽  
pp. 394-404 ◽  
Author(s):  
Francis P. Wong ◽  
Wayne F. Wilcox
Keyword(s):  
New York ◽  
High Sensitivity ◽  
Intrinsic Activity ◽  
Uncinula Necator ◽  
In Planta ◽  
Baseline Sensitivity ◽  
Sensitivity Distribution ◽  
Resistant Population ◽  
History Of ◽  
Low Sensitivity

Two hundred fifty-six single-conidial chain isolates of Uncinula necator were assayed for their sensitivity to azoxystrobin and myclobutanil. These isolates were collected from two sites in New York in 1999: an “organic” vineyard where no synthetic fungicides have been used (baseline population) and a commercial vineyard having a history of compromised powdery mildew control with myclobutanil (demethylation inhibitor [DMI]-resistant population). Mean coefficients of variance for a leaf disk assay used to test fungicide sensitivities were 31% for azoxystrobin and 41% for myclobutanil. Baseline ED50 values ranged from 0.0037 to 0.028 μg/ml (mean 0.0097μg/ml) for azoxystrobin and from 0.0049 to 0.69 μg/ml (mean 0.075 μg/ml) for myclobutanil. A shift in the mean ED50 value for azoxystrobin to 0.018 μg/ml was observed in the DMI-resistant population; with the strongest shift observed for isolates collected from vines treated exclusively with myclobutanil (0.024 μg/ml). For the 256 tested isolates, there was a moderate, but statistically significant, correlation between azoxystrobin and myclobutanil sensitivities (R2 = 0.36, P < 0.001). Tests with three other strobilurin fungicides (kresoxim-methyl, pyraclostrobin, and trifloxystrobin) indicate clear differences in the intrinsic activity of these compounds against U. necator, and the applicability of the methods developed with azoxystrobin for assays with pyraclostrobin and trifloxystrobin. Isolates from the high and low ends of the azoxystrobin sensitivity distribution (15× difference in mean ED50 values) were equally controlled in planta by protectant or postinfection treatment with azoxystrobin at 250 μg a.i./ml, but postinfection application at lower rates (2.5 and 25 μg a.i./ml) resulted in a 41 and 44% decrease, respectively, in the control of the low-sensitivity isolates versus high-sensitivity isolates. The results of this study document the baseline sensitivity distribution of U. necator to azoxystrobin, provide evidence of partial cross-sensitivity between azoxystrobin and myclobutanil, and illustrate the potential selection for individuals with reduced sensitivity (quantitative range) to azoxystrobin by postinfection application and reduced rates of this fungicide.

scholarly journals Optical Aggregation of Gold Nanoparticles for SERS Detection of Proteins and Toxins in Liquid Environment: Towards Ultrasensitive and Selective Detection

2018 ◽  
Author(s):  
Antonino Foti ◽  
Cristiano D'andrea ◽  
Valentina Villari ◽  
Norberto Micali ◽  
Maria Grazia Donato ◽  
...  
Keyword(s):  
Hot Spots ◽  
High Sensitivity ◽  
Quantitative Detection ◽  
Selective Detection ◽  
Liquid Environment ◽  
Fungal Toxin ◽  
First Results ◽  
Sers Detection ◽  
Molecular Specificity ◽  
Food Commodities

Optical forces are used to aggregate plasmonic nanoparticles and create SERS-active hot spots in liquid. When biomolecules are added to the nanoparticles, high sensitivity SERS detection is accomplished. Here we tailor this methodology to detect catalase and hemoglobin, two Raman resonant biomolecules, at concentrations down to 10 nM and 1 pM. Subsequently, we study the SERS signal in Bovine Serum Albumin as a function of the concentration, finding a monotonic dependence that suggests the possibility of quantitative detection. Finally, by exploiting nanoparticles functionalized with specific aptamers, we obtain first results on the SERS detection of Ochratoxin A, a fungal toxin found in food commodities and wine. This represents a first step towards the addition of molecular specificity to this novel biosensor strategy.

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