Linear single-labeled DNA probes are used in this RCA-based fluorescence strategy for DNA detection, which could effectively avoid the fluorescence quenching between neighboring signal probes using hairpin probe as signal probe.
A sensitive and label-free DNA detection method was developed based on cascade amplification combining exonuclease-III recycling with rolling circle amplification.
An integrated gold electrode fabricated on a polystyrene substrate was developed for quantitative detection of nucleic acids by rolling circle amplification and chronocoulometry.