An ultrasensitive fluorometric platform for S1 nuclease assay based on cytochrome c

RSC Advances ◽  
2014 ◽  
Vol 4 (67) ◽  
pp. 35422-35427 ◽  
Author(s):  
Yue He ◽  
Bining Jiao
Keyword(s):  
Cytochrome C ◽  
Nuclease Activity ◽  
S1 Nuclease ◽  
Fluorescent Sensing ◽  
Sensing Platform ◽  
Nuclease Assay

An ultrasensitive and straightforward fluorescent sensing platform for S1 nuclease activity has been developed based on cytochrome c.

Fluorescent S1 nuclease assay utilizing exponential strand displacement amplification

The Analyst ◽  
2019 ◽  
Vol 144 (10) ◽  
pp. 3364-3368 ◽  
Author(s):  
Chang Yeol Lee ◽  
Hansol Kim ◽  
Hyo Yong Kim ◽  
Ki Soo Park ◽  
Hyun Gyu Park
Keyword(s):  
Nuclease Activity ◽  
Label Free ◽  
Strand Displacement ◽  
Activity Assay ◽  
Strand Displacement Amplification ◽  
S1 Nuclease ◽  
Nuclease Assay

We devise a simple, label-free S1 nuclease activity assay by exploiting target-induced inhibition of exponential strand displacement amplification (eSDA).

Phosphorylation-induced formation of a cytochrome c-peptide complex: a novel fluorescent sensing platform for protein kinase assay

2016 ◽  
Vol 52 (4) ◽  
pp. 776-779 ◽  
Author(s):  
Shuang Wu ◽  
Xiang-Juan Kong ◽  
Yao Cen ◽  
Ru-Qin Yu ◽  
Xia Chu
Keyword(s):  
Protein Kinase ◽  
Cytochrome C ◽  
Kinase Assay ◽  
C Peptide ◽  
Peptide Complex ◽  
Fluorescent Sensing ◽  
Sensing Platform

A novel fluorescent sensing platform has been developed for protein kinase assay based on the phosphorylation-induced formation of a cytochrome c-peptide complex.

A dual-amplification fluorescent sensing platform for ultrasensitive assay of nuclease and ATP based on rolling circle replication and exonuclease III-aided recycling

RSC Advances ◽  
2015 ◽  
Vol 5 (92) ◽  
pp. 75055-75061 ◽  
Author(s):  
Chen-Liwei Liu ◽  
Xiang-Juan Kong ◽  
Jing Yuan ◽  
Ru-Qin Yu ◽  
Xia Chu
Keyword(s):  
Rolling Circle Replication ◽  
S1 Nuclease ◽  
Exonuclease Iii ◽  
Rolling Circle ◽  
Fluorescent Sensing ◽  
Sensing Platform ◽  
Ultrasensitive Assay ◽  
Exo Iii ◽  
Dual Amplification ◽  
Amplification Strategy

A ultrasensitive, easy operated and robust assay of S1 nuclease in real samples and ATP has been successfully achieved with the dual-amplification strategy based on rolling circle replication and Exo III-aided recycling.

scholarly journals A multicolor and ratiometric fluorescent sensing platform for metal ions based on arene–metal-ion contact

2021 ◽  
Vol 4 (1) ◽  
Author(s):  
Anna Kanegae ◽  
Yusuke Takata ◽  
Ippei Takashima ◽  
Shohei Uchinomiya ◽  
Ryosuke Kawagoe ◽  
...  
Keyword(s):  
Metal Ions ◽  
Metal Ion ◽  
Near Infrared ◽  
Molecular Design ◽  
Theoretical Calculations ◽  
Probe Design ◽  
One Pot ◽  
Fluorescent Sensing ◽  
Sensing Platform ◽  
Ratiometric Detection

AbstractDespite continuous and active development of fluorescent metal-ion probes, their molecular design for ratiometric detection is restricted by the limited choice of available sensing mechanisms. Here we present a multicolor and ratiometric fluorescent sensing platform for metal ions based on the interaction between the metal ion and the aromatic ring of a fluorophore (arene–metal-ion, AM, coordination). Our molecular design provided the probes possessing a 1,9-bis(2′-pyridyl)-2,5,8-triazanonane as a flexible metal ion binding unit attached to a tricyclic fluorophore. This architecture allows to sense various metal ions, such as Zn(II), Cu(II), Cd(II), Ag(I), and Hg(II) with emission red-shifts. We showed that this probe design is applicable to a series of tricyclic fluorophores, which allow ratiometric detection of the metal ions from the blue to the near-infrared wavelengths. X-ray crystallography and theoretical calculations indicate that the coordinated metal ion has van der Waals contact with the fluorophore, perturbing the dye’s electronic structure and ring conformation to induce the emission red-shift. A set of the probes was useful for the differential sensing of eight metal ions in a one-pot single titration via principal component analysis. We also demonstrate that a xanthene fluorophore is applicable to the ratiometric imaging of metal ions under live-cell conditions.

An efficient fluorescent sensing platform for biomolecules based on fenton reaction triggered molecular beacon cleavage strategy

2013 ◽  
Vol 41 ◽  
pp. 442-445 ◽  
Author(s):  
Rong Hu ◽  
Ya-Ru Liu ◽  
Xiao-Bing Zhang ◽  
Weihong Tan ◽  
Guo-Li Shen ◽  
...  
Keyword(s):  
Fenton Reaction ◽  
Molecular Beacon ◽  
Fluorescent Sensing ◽  
Sensing Platform

Integrating dye-intercalated DNA dendrimers with electrospun nanofibers: a new fluorescent sensing platform for nucleic acids, proteins, and cells

2015 ◽  
Vol 3 (17) ◽  
pp. 3541-3547 ◽  
Author(s):  
Huaming Wang ◽  
Wei Tang ◽  
Hejia Wei ◽  
Yan Zhao ◽  
Shichao Hu ◽  
...  
Keyword(s):  
Nucleic Acids ◽  
Related Species ◽  
Electrospun Nanofibers ◽  
Fluorescent Dye ◽  
Fluorescent Sensing ◽  
Sensing Platform

A fluorescent dye-intercalated DNA dendrimer probe was integrated with electrospun nanofibers to create an amplified sensing platform for disease-related species.

CeO2 Nanowire-BODIPY-Adenosine Triphosphate Fluorescent Sensing Platform for Highly Specific and Sensitive Detection of Arsenate

2018 ◽  
Vol 90 (24) ◽  
pp. 14507-14513 ◽  
Author(s):  
Lili Tong ◽  
Xiuxiu Wang ◽  
Wen Gao ◽  
Zhenhua Liu ◽  
Zhenzhen Chen ◽  
...  
Keyword(s):  
Adenosine Triphosphate ◽  
Sensitive Detection ◽  
Fluorescent Sensing ◽  
Sensing Platform
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