Functional investigations on embryonic stem cells labeled with clinically translatable iron oxide nanoparticles

Nanoscale ◽  
2014 ◽  
Vol 6 (15) ◽  
pp. 9025 ◽  
Author(s):  
Jing Liu ◽  
Liqin Wang ◽  
Jianbo Cao ◽  
Yue Huang ◽  
Yu Lin ◽  
...  
Keyword(s):  
Stem Cells ◽  
Iron Oxide ◽  
Embryonic Stem Cells ◽  
Iron Oxide Nanoparticles ◽  
Embryonic Stem ◽  
Oxide Nanoparticles

Effects of iron oxide nanoparticles on cardiac differentiation of embryonic stem cells

2009 ◽  
Vol 379 (4) ◽  
pp. 898-903 ◽  
Author(s):  
Ka-Wing Au ◽  
Song-Yan Liao ◽  
Yee-Ki Lee ◽  
Wing-Hon Lai ◽  
Kwong-Man Ng ◽  
...  
Keyword(s):  
Stem Cells ◽  
Iron Oxide ◽  
Embryonic Stem Cells ◽  
Iron Oxide Nanoparticles ◽  
Embryonic Stem ◽  
Cardiac Differentiation ◽  
Oxide Nanoparticles

Magnetic resonance tracking of transplanted bone marrow and embryonic stem cells labeled by iron oxide nanoparticles in rat brain and spinal cord

2004 ◽  
Vol 76 (2) ◽  
pp. 232-243 ◽  
Author(s):  
Pavla Jendelová ◽  
Vít Herynek ◽  
Lucia Urdzíková ◽  
Kateřina Glogarová ◽  
Jana Kroupová ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Stem Cells ◽  
Bone Marrow ◽  
Iron Oxide ◽  
Embryonic Stem Cells ◽  
Magnetic Resonance ◽  
Rat Brain ◽  
Embryonic Stem ◽  
Oxide Nanoparticles ◽  
Brain And Spinal Cord

scholarly journals Mesenchymal Stem Cells Do Not Lose Direct Labels Including Iron Oxide Nanoparticles and DFO-89Zr Chelates through Secretion of Extracellular Vesicles

Membranes ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 484
Author(s):  
Yue Gao ◽  
Anna Jablonska ◽  
Chengyan Chu ◽  
Piotr Walczak ◽  
Miroslaw Janowski
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Iron Oxide ◽  
Iron Oxide Nanoparticles ◽  
Extracellular Vesicles ◽  
Superparamagnetic Iron Oxide ◽  
Cell Labeling ◽  
Oxide Nanoparticles ◽  
Stem Cell Delivery ◽  
Cellular Labeling

Rapidly ageing populations are beset by tissue wear and damage. Stem cell-based regenerative medicine is considered a solution. Years of research point to two important aspects: (1) the use of cellular imaging to achieve sufficient precision of therapeutic intervention, and the fact that (2) many therapeutic actions are executed through extracellular vesicles (EV), released by stem cells. Therefore, there is an urgent need to interrogate cellular labels in the context of EV release. We studied clinically applicable cellular labels: superparamagnetic iron oxide nanoparticles (SPION), and radionuclide detectable by two main imaging modalities: MRI and PET. We have demonstrated effective stem cell labeling using both labels. Then, we obtained EVs from cell cultures and tested for the presence of cellular labels. We did not find either magnetic or radioactive labels in EVs. Therefore, we report that stem cells do not lose labels in released EVs, which indicates the reliability of stem cell magnetic and radioactive labeling, and that there is no interference of labels with EV content. In conclusion, we observed that direct cellular labeling seems to be an attractive approach to monitoring stem cell delivery, and that, importantly, labels neither locate in EVs nor affect their basic properties.

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