scholarly journals On-demand optical immobilization of Caenorhabditis elegans for high-resolution imaging and microinjection

Lab on a Chip ◽  
2014 ◽  
Vol 14 (18) ◽  
pp. 3498 ◽  
Author(s):  
Hyundoo Hwang ◽  
Jan Krajniak ◽  
Yohei Matsunaga ◽  
Guy M. Benian ◽  
Hang Lu
Keyword(s):  
Caenorhabditis Elegans ◽  
High Resolution ◽  
High Resolution Imaging ◽  
On Demand ◽  
Resolution Imaging

On-demand dielectrophoretic immobilization and high-resolution imaging of C. elegans in microfluids

2018 ◽  
Vol 259 ◽  
pp. 703-708 ◽  
Author(s):  
Liang Huang ◽  
Peng Zhao ◽  
Junhan Wu ◽  
Han-Sheng Chuang ◽  
Wenhui Wang
Keyword(s):  
High Resolution ◽  
High Resolution Imaging ◽  
C Elegans ◽  
On Demand ◽  
Resolution Imaging

scholarly journals Assembly of Caenorhabditis elegans acentrosomal spindles occurs without evident microtubule-organizing centers and requires microtubule sorting by KLP-18/kinesin-12 and MESP-1

2016 ◽  
Vol 27 (20) ◽  
pp. 3122-3131 ◽  
Author(s):  
Ian D. Wolff ◽  
Michael V. Tran ◽  
Timothy J. Mullen ◽  
Anne M. Villeneuve ◽  
Sarah M. Wignall
Keyword(s):  
Caenorhabditis Elegans ◽  
High Resolution ◽  
Nuclear Envelope ◽  
Spindle Assembly ◽  
Cell Types ◽  
High Resolution Imaging ◽  
Spindle Formation ◽  
Mixed Polarity ◽  
Resolution Imaging ◽  
Meiosis I

Although centrosomes contribute to spindle formation in most cell types, oocytes of many species are acentrosomal and must organize spindles in their absence. Here we investigate this process in Caenorhabditis elegans, detailing how acentrosomal spindles form and revealing mechanisms required to establish bipolarity. Using high-resolution imaging, we find that in meiosis I, microtubules initially form a “cage-like” structure inside the disassembling nuclear envelope. This structure reorganizes so that minus ends are sorted to the periphery of the array, forming multiple nascent poles that then coalesce until bipolarity is achieved. In meiosis II, microtubules nucleate in the vicinity of chromosomes but then undergo similar sorting and pole formation events. We further show that KLP-18/kinesin-12 and MESP-1, previously shown to be required for spindle bipolarity, likely contribute to bipolarity by sorting microtubules. After their depletion, minus ends are not sorted outward at the early stages of spindle assembly and instead converge. These proteins colocalize on microtubules, are interdependent for localization, and can interact, suggesting that they work together. We propose that KLP-18/kinesin-12 and MESP-1 form a complex that functions to sort microtubules of mixed polarity into a configuration in which minus ends are away from the chromosomes, enabling formation of nascent poles.

scholarly journals Hydrogel-droplet microfluidic platform for high-resolution imaging and sorting of early larval Caenorhabditis elegans

Lab on a Chip ◽  
2015 ◽  
Vol 15 (6) ◽  
pp. 1424-1431 ◽  
Author(s):  
Guillaume Aubry ◽  
Mei Zhan ◽  
Hang Lu
Keyword(s):  
Caenorhabditis Elegans ◽  
High Resolution ◽  
Microfluidic Device ◽  
High Resolution Imaging ◽  
Microfluidic Platform ◽  
C Elegans ◽  
Resolution Imaging

We present a microfluidic device for high-resolution imaging and sorting of early larval C. elegans. The animals are isolated in droplets and temporarily immobilized for imaging using a reversible hydrogel.

scholarly journals Pneumatic stimulation of C. elegans mechanoreceptor neurons in a microfluidic trap

Lab on a Chip ◽  
2017 ◽  
Vol 17 (6) ◽  
pp. 1116-1127 ◽  
Author(s):  
Adam L. Nekimken ◽  
Holger Fehlauer ◽  
Anna A. Kim ◽  
Sandra N. Manosalvas-Kjono ◽  
Purim Ladpli ◽  
...  
Keyword(s):  
Caenorhabditis Elegans ◽  
High Resolution ◽  
Neuronal Activity ◽  
High Resolution Imaging ◽  
C Elegans ◽  
Resolution Imaging ◽  
Simultaneous Immobilization ◽  
Stimulation Of

A new microfluidic tool for simultaneous immobilization, force delivery and high resolution imaging of neuronal activity in living Caenorhabditis elegans.

scholarly journals Reversible and long-term immobilization in a hydrogel-microbead matrix for high-resolution imaging of Caenorhabditis elegans and other small organisms

PLoS ONE ◽  
2018 ◽  
Vol 13 (3) ◽  
pp. e0193989 ◽  
Author(s):  
Li Dong ◽  
Matteo Cornaglia ◽  
Gopalan Krishnamani ◽  
Jingwei Zhang ◽  
Laurent Mouchiroud ◽  
...  
Keyword(s):  
Caenorhabditis Elegans ◽  
High Resolution ◽  
High Resolution Imaging ◽  
Resolution Imaging

Alternative approaches to ultra-high resolution imaging

1991 ◽  
Vol 49 ◽  
pp. 650-651
Author(s):  
J.M. Cowley
Keyword(s):  
High Resolution ◽  
High Voltage ◽  
High Resolution Electron Microscopy ◽  
Crystal Defects ◽  
High Resolution Imaging ◽  
General Applicability ◽  
Resolution Electron ◽  
Radiation Resistant ◽  
Resolution Imaging ◽  
Alternative Approaches

By extrapolation of past experience, it would seem that the future of ultra-high resolution electron microscopy rests with the advances of electron optical engineering that are improving the instrumental stability of high voltage microscopes to achieve the theoretical resolutions of 1Å or better at 1MeV or higher energies. While these high voltage instruments will undoubtedly produce valuable results on chosen specimens, their general applicability has been questioned on the basis of the excessive radiation damage effects which may significantly modify the detailed structures of crystal defects within even the most radiation resistant materials in a period of a few seconds. Other considerations such as those of cost and convenience of use add to the inducement to consider seriously the possibilities for alternative approaches to the achievement of comparable resolutions.

Principle and practice of high-resolution imaging with a field-emission TEM

1992 ◽  
Vol 50 (1) ◽  
pp. 138-139
Author(s):  
Max T. Otten ◽  
Wim M.J. Coene
Keyword(s):  
High Resolution ◽  
Field Emission ◽  
Incidence Angle ◽  
Temporal Coherence ◽  
Energy Spread ◽  
High Resolution Imaging ◽  
Major Improvement ◽  
High Resolution Images ◽  
Resolution Imaging

High-resolution imaging with a LaB6 instrument is limited by the spatial and temporal coherence, with little contrast remaining beyond the point resolution. A Field Emission Gun (FEG) reduces the incidence angle by a factor 5 to 10 and the energy spread by 2 to 3. Since the incidence angle is the dominant limitation for LaB6 the FEG provides a major improvement in contrast transfer, reducing the information limit to roughly one half of the point resolution. The strong improvement, predicted from high-resolution theory, can be seen readily in diffractograms (Fig. 1) and high-resolution images (Fig. 2). Even if the information in the image is limited deliberately to the point resolution by using an objective aperture, the improved contrast transfer close to the point resolution (Fig. 1) is already worthwhile.

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