A single-phase flow microfluidic cell sorter for multiparameter screening to assist the directed evolution of Ca2+ sensors

Lab on a Chip ◽  
2019 ◽  
Vol 19 (22) ◽  
pp. 3880-3887 ◽  
Author(s):  
Yufeng Zhao ◽  
Wei Zhang ◽  
Yongxin Zhao ◽  
Robert E. Campbell ◽  
D. Jed Harrison
Keyword(s):  
Directed Evolution ◽  
Single Phase ◽  
Fluorescent Protein ◽  
Detection System ◽  
Phase Flow ◽  
Bacterial Cells ◽  
Single Phase Flow ◽  
Cell Sorter ◽  
Point Detection ◽  
Microfluidic Cell Sorter

We introduce a single-phase flow microfluidic cell sorter with a two-point detection system capable of two-parameter screening to assist with directed evolution of a fluorescent protein based Ca2+ sensor expressed in bacterial cells.

Critical review on wax deposition in single-phase flow

Fuel ◽  
2021 ◽  
Vol 293 ◽  
pp. 120358
Author(s):  
Charlie van der Geest ◽  
Aline Melchuna ◽  
Letícia Bizarre ◽  
Antonio C. Bannwart ◽  
Vanessa C.B. Guersoni
Keyword(s):  
Critical Review ◽  
Single Phase ◽  
Phase Flow ◽  
Single Phase Flow ◽  
Wax Deposition

Flow Patterns During Flow Boiling Instability in Silicon-Based Pin-Fin Microchannels

2017 ◽  
Vol 140 (3) ◽  
Author(s):  
Fayao Xu ◽  
Huiying Wu ◽  
Zhenyu Liu
Keyword(s):  
Single Phase ◽  
Flow Instability ◽  
Flow Boiling ◽  
Flow Patterns ◽  
Phase Flow ◽  
Single Phase Flow ◽  
Pin Fin ◽  
Short Period ◽  
Pin Fins ◽  
Amplitude Mode

In this paper, the flow patterns during water flow boiling instability in pin-fin microchannels were experimentally studied. Three types of pin-fin arrays (in-line/circular pin-fins, staggered/circular pin-fins, and staggered/square pin-fins) were used in the study. The flow instability started to occur as the outlet water reached the saturation temperature. Before the unstable boiling, a wider range of stable boiling existed in the pin-fin microchannels compared to that in the plain microchannels. Two flow instability modes for the temperature and pressure oscillations, which were long-period/large-amplitude mode and short-period/small-amplitude mode, were identified. The temperature variation during the oscillation period of the long-period/large-amplitude mode can be divided into two stages: increasing stage and decreasing stage. In the increasing stage, bubbly flow, vapor-slug flow, stratified flow, and wispy flow occurred sequentially with time for the in-line pin-fin microchannels; liquid single-phase flow, aforementioned four kinds of two-phase flow patterns, and vapor single-phase flow occurred sequentially with time for the staggered pin-fin microchannel. The flow pattern transitions in the decreasing stage were the inverse of those in the increasing stage for both in-line and staggered pin-fin microchannels. For the short-period/small-amplitude oscillation mode, only the wispy flow occurred. With the increase of heat flux, the wispy flow and the vapor single-phase flow occupied more and more time ratio during an oscillation period in the in-line and staggered pin-fin microchannels.

scholarly journals Nonlinear single-phase flow in real rock joints

2007 ◽  
Vol 43 (9) ◽  
Author(s):  
P. G. Ranjith ◽  
W. Darlington
Keyword(s):  
Single Phase ◽  
Rock Joints ◽  
Phase Flow ◽  
Single Phase Flow
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