scholarly journals DNA fragment conformations in adducts with Kiteplatin

2015 ◽  
Vol 44 (8) ◽  
pp. 3544-3556 ◽  
Author(s):  
Nicola Margiotta ◽  
Emanuele Petruzzella ◽  
James A. Platts ◽  
Shaun T. Mutter ◽  
Robert J. Deeth ◽  
...  
Keyword(s):  
Platinum Complex ◽  
Dna Fragment ◽  
Multiple Conformers

The anticancer-active platinum complex with cis-1,4-diaminocyclohexane has proved to be very valuable in detecting multiple conformers in adducts with oligonucleotides.

A Study of DNA Fragment Assembly Algorithms

10.26524/jap2 ◽  
2016 ◽  
Vol 1 (1) ◽  
pp. 10-16
Author(s):  
Satyanarayana Reddy Beeram ◽  
Edara Srinivasa Reddy
Keyword(s):  
Fragment Assembly ◽  
Dna Fragment ◽  
Assembly Algorithms ◽  
Dna Fragment Assembly

scholarly journals The Cloning and Molecular Analysis of pawn-B in Paramecium tetraurelia

Genetics ◽  
2000 ◽  
Vol 155 (3) ◽  
pp. 1105-1117 ◽  
Author(s):  
W John Haynes ◽  
Kit-Yin Ling ◽  
Robin R Preston ◽  
Yoshiro Saimi ◽  
Ching Kung
Keyword(s):  
Genomic Library ◽  
Open Reading Frame ◽  
Paramecium Tetraurelia ◽  
Wild Type ◽  
Rt Pcr ◽  
Reading Frame ◽  
Close Proximity ◽  
In The Wild ◽  
Dna Fragment ◽  
Alternative Sites

Abstract Pawn mutants of Paramecium tetraurelia lack a depolarization-activated Ca2+ current and do not swim backward. Using the method of microinjection and sorting a genomic library, we have cloned a DNA fragment that complements pawn-B (pwB/pwB). The minimal complementing fragment is a 798-bp open reading frame (ORF) that restores the Ca2+ current and the backward swimming when expressed. This ORF contains a 29-bp intron and is transcribed and translated. The translated product has two putative transmembrane domains but no clear matches in current databases. Mutations in the available pwB alleles were found within this ORF. The d4-95 and d4-96 alleles are single base substitutions, while d4-662 (previously pawn-D) harbors a 44-bp insertion that matches an internal eliminated sequence (IES) found in the wild-type germline DNA except for a single C-to-T transition. Northern hybridizations and RT-PCR indicate that d4-662 transcripts are rapidly degraded or not produced. A second 155-bp IES in the wild-type germline ORF excises at two alternative sites spanning three asparagine codons. The pwB ORF appears to be separated from a 5′ neighboring ORF by only 36 bp. The close proximity of the two ORFs and the location of the pwB protein as indicated by GFP-fusion constructs are discussed.

Highly repeated DNA sequences in birds: The structure and evolution of an abundant, tandemly repeated 190-bp DNA fragment in parrots

Genomics ◽  
1992 ◽  
Vol 14 (2) ◽  
pp. 462-469 ◽  
Author(s):  
Cort S. Madsen ◽  
Dineke H. de Kloet ◽  
Jean E. Brooks ◽  
Siwo R. de Kloet
Keyword(s):  
Dna Sequences ◽  
Repeated Dna ◽  
Repeated Dna Sequences ◽  
Highly Repeated Dna ◽  
Dna Fragment

scholarly journals Engineering nucleosomes for generating diverse chromatin assemblies

2021 ◽  
Author(s):  
Zenita Adhireksan ◽  
Deepti Sharma ◽  
Phoi Leng Lee ◽  
Qiuye Bao ◽  
Sivaraman Padavattan ◽  
...  
Keyword(s):  
Dynamic Properties ◽  
Dna Nanostructures ◽  
Macromolecular Assemblies ◽  
Maximum Resolution ◽  
Different Types ◽  
Chromatin Structural ◽  
Dna Fragment

Abstract Structural characterization of chromatin is challenging due to conformational and compositional heterogeneity in vivo and dynamic properties that limit achievable resolution in vitro. Although the maximum resolution for solving structures of large macromolecular assemblies by electron microscopy has recently undergone profound increases, X-ray crystallographic approaches may still offer advantages for certain systems. One such system is compact chromatin, wherein the crystalline state recapitulates the crowded molecular environment within the nucleus. Here we show that nucleosomal constructs with cohesive-ended DNA can be designed that assemble into different types of circular configurations or continuous fibers extending throughout crystals. We demonstrate the utility of the method for characterizing nucleosome compaction and linker histone binding at near-atomic resolution but also advance its application for tackling further problems in chromatin structural biology and for generating novel types of DNA nanostructures. We provide a library of cohesive-ended DNA fragment expression constructs and a strategy for engineering DNA-based nanomaterials with a seemingly vast potential variety of architectures and histone chemistries.

The clp1 Gene of the Mushroom Coprinus cinereus Is Essential for A-Regulated Sexual Development

Genetics ◽  
2001 ◽  
Vol 157 (1) ◽  
pp. 133-140
Author(s):  
Kazumi Inada ◽  
Yoshinori Morimoto ◽  
Toshihide Arima ◽  
Yukio Murata ◽  
Takashi Kamada
Keyword(s):  
Sexual Development ◽  
Genomic Dna ◽  
Mutant Allele ◽  
Coprinus Cinereus ◽  
Mating Partner ◽  
Essential Step ◽  
Genes Encoding ◽  
Dna Fragment ◽  
Necessary And Sufficient ◽  
Novel Protein

Abstract Sexual development in the mushroom Coprinus cinereus is under the control of the A and B mating-type loci, both of which must be different for a compatible, dikaryotic mycelium to form between two parents. The A genes, encoding proteins with homeodomain motifs, regulate conjugate division of the two nuclei from each mating partner and promote the formation of clamp connections. The latter are hyphal configurations required for the maintenance of the nuclear status in the dikaryotic phase of basidiomycetes. The B genes encode pheromones and pheromone receptors. They regulate the cellular fusions that complete clamp connections during growth, as well as the nuclear migration required for dikaryosis. The AmutBmut strain (326) of C. cinereus, in which both A- and B-regulated pathways are constitutively activated by mutations, produces, without mating, dikaryon-like, fertile hyphae with clamp connections. In this study we isolated and characterized clampless1-1 (clp1-1), a mutation that blocks clamp formation, an essential step in A-regulated sexual development, in the AmutBmut background. A genomic DNA fragment that rescues the clp1-1 mutation was identified by transformations. Sequencing of the genomic DNA, together with RACE experiments, identified an ORF interrupted by one intron, encoding a novel protein of 365 amino acids. The clp1-1 mutant allele carries a deletion of four nucleotides, which is predicted to cause elimination of codon 128 and frameshifts thereafter. The clp1 transcript was normally detected only in the presence of the A protein heterodimer formed when homokaryons with compatible A genes were mated. Forced expression of clp1 by promoter replacements induced clamp development without the need for a compatible A gene combination. These results indicate that expression of clp1 is necessary and sufficient for induction of the A-regulated pathway that leads to clamp development.

scholarly journals P‐13.3: Efficient Blue and Color Tunable White OLED Based on Platinum Complex

2021 ◽  
Vol 52 (S1) ◽  
pp. 637-637
Author(s):  
Jianfeng Guo ◽  
Zhangcheng Liao ◽  
Rongrong Xia ◽  
Hong Lin ◽  
Zixing Wang
Keyword(s):  
Platinum Complex ◽  
Color Tunable ◽  
White Oled

scholarly journals Activation of a cryptic gene by excision of a DNA fragment.

1988 ◽  
Vol 170 (1) ◽  
pp. 218-222 ◽  
Author(s):  
L L Parker ◽  
P W Betts ◽  
B G Hall
Keyword(s):  
Dna Fragment

scholarly journals Selective enrichment of a large size genomic DNA fragment by affinity capture: an approach for genome mapping

1990 ◽  
Vol 18 (7) ◽  
pp. 1789-1795 ◽  
Author(s):  
Rajendra P. Kandpal ◽  
David C. Ward ◽  
Sherman M. Weissman
Keyword(s):  
Genomic Dna ◽  
Genome Mapping ◽  
Affinity Capture ◽  
Selective Enrichment ◽  
Large Size ◽  
Dna Fragment
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