A novel fluorescence probe for estimation of cysteine/histidine in human blood plasma and recognition of endogenous cysteine in live Hct116 cells

2014 ◽  
Vol 50 (69) ◽  
pp. 9899-9902 ◽  
Author(s):  
Upendar Reddy G. ◽  
Hridesh Agarwalla ◽  
Nandaraj Taye ◽  
Suvankar Ghorai ◽  
Samit Chattopadhyay ◽  
...  
Keyword(s):  
Cell Membrane ◽  
Blood Plasma ◽  
Human Blood ◽  
Plasma Sample ◽  
Fluorescence Probe ◽  
Quantitative Estimation ◽  
Human Blood Plasma ◽  
Blood Plasma Sample ◽  
Hct116 Cells

A new cell membrane permeable reagent for detection of endogenous Cys as well as quantitative estimation of Cys and His in a human blood plasma sample.

A fluorescent probe for specific detection of cysteine in the lipid dense region of cells

2015 ◽  
Vol 51 (95) ◽  
pp. 16932-16935 ◽  
Author(s):  
Firoj Ali ◽  
Anila H. A. ◽  
Nandaraj Taye ◽  
Rajesh G. Gonnade ◽  
Samit Chattopadhyay ◽  
...  
Keyword(s):  
Drug Metabolism ◽  
Blood Plasma ◽  
Human Blood ◽  
Hepg2 Cells ◽  
Plasma Sample ◽  
Quantitative Estimation ◽  
Human Blood Plasma ◽  
Dense Region ◽  
Specific Detection ◽  
Blood Plasma Sample

Use of a cysteine specific chemodosimetric receptor for probing its release in the ER-region of the HepG2 cells during drug metabolism and silica based strips for quantitative estimation of Cys in a human blood plasma sample.

Dual Layer Mixed Matrix Hollow Fiber Membranes for Outside-In Filtration of Human Blood Plasma

2020 ◽  
Author(s):  
O.E.M. ter Beek ◽  
M.K. van Gelder ◽  
C. Lokhorst ◽  
D.H.M. Hazenbrink ◽  
B.H. Lentferink ◽  
...  
Keyword(s):  
Blood Plasma ◽  
Human Blood ◽  
Hollow Fiber ◽  
Human Blood Plasma ◽  
Hollow Fiber Membranes ◽  
Mixed Matrix ◽  
Fiber Membranes

Detectability of Plasma Proteins in SRM Measurements

2018 ◽  
Vol 16 (1) ◽  
pp. 74-81 ◽  
Author(s):  
Olga I. Kiseleva ◽  
Elena A. Ponomarenko ◽  
Yulia A. Romashova ◽  
Ekaterina V. Poverennaya ◽  
Andrey V. Lisitsa
Keyword(s):  
Blood Plasma ◽  
Human Plasma ◽  
Limit Of Detection ◽  
Human Blood Plasma ◽  
Analytical Sensitivity ◽  
Plasma Proteome ◽  
Protein Targets ◽  
Blood Plasma Sample ◽  
Specificity And Sensitivity ◽  
Human Plasma Proteome

Background: Liquid chromatography coupled with targeted mass spectrometry underwent rapid technical evolution during last years and has become widely used technology in clinical laboratories. It offers confident specificity and sensitivity superior to those of traditional immunoassays. However, due to controversial reports on reproducibility of SRM measurements, the prospects of clinical appliance of the method are worth discussing. </P><P> Objective: The study was aimed at assessment of capabilities of SRM to achieve a thorough assembly of the human plasma proteome. </P><P> Method: We examined set of 19 human blood plasma samples to measure 100 proteins, including FDA-approved biomarkers, via SRM-assay. </P><P> Results: Out of 100 target proteins 43 proteins were confidently detected in at least two blood plasma sample runs, 36 and 21 proteins were either not detected in any run or inconsistently detected, respectively. Empiric dependences on protein detectability were derived to predict the number of biological samples required to detect with certainty a diagnostically relevant quantum of the human plasma proteome. </P><P> Conclusion: The number of samples exponentially increases with an increase in the number of protein targets, while proportionally decreasing to the logarithm of the limit of detection. Analytical sensitivity and enormous proteome heterogeneity are major bottlenecks of the human proteome exploration.

scholarly journals Properties of triglyceride-rich and cholesterol-rich lipoproteins in the remnant-like particle fraction of human blood plasma

2002 ◽  
Vol 43 (3) ◽  
pp. 365-374
Author(s):  
Elisa Campos ◽  
Leila Kotite ◽  
Patricia Blanche ◽  
Yasushi Mitsugi ◽  
Philip H. Frost ◽  
...  
Keyword(s):  
Blood Plasma ◽  
Human Blood ◽  
Human Blood Plasma ◽  
Particle Fraction
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