Investigation of Human Blood Plasma Sample Preparation for Performing Metabolomics Using Ultrahigh Performance Liquid Chromatography/Mass Spectrometry

2009 ◽  
Vol 81 (9) ◽  
pp. 3285-3296 ◽  
Author(s):  
Stephen J. Bruce ◽  
Isabelle Tavazzi ◽  
Véronique Parisod ◽  
Serge Rezzi ◽  
Sunil Kochhar ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Sample Preparation ◽  
Blood Plasma ◽  
Human Blood ◽  
Plasma Sample ◽  
Human Blood Plasma ◽  
Liquid Chromatography Mass Spectrometry ◽  
Blood Plasma Sample ◽  
Ultrahigh Performance Liquid Chromatography

A fluorescent probe for specific detection of cysteine in the lipid dense region of cells

2015 ◽  
Vol 51 (95) ◽  
pp. 16932-16935 ◽  
Author(s):  
Firoj Ali ◽  
Anila H. A. ◽  
Nandaraj Taye ◽  
Rajesh G. Gonnade ◽  
Samit Chattopadhyay ◽  
...  
Keyword(s):  
Drug Metabolism ◽  
Blood Plasma ◽  
Human Blood ◽  
Hepg2 Cells ◽  
Plasma Sample ◽  
Quantitative Estimation ◽  
Human Blood Plasma ◽  
Dense Region ◽  
Specific Detection ◽  
Blood Plasma Sample

Use of a cysteine specific chemodosimetric receptor for probing its release in the ER-region of the HepG2 cells during drug metabolism and silica based strips for quantitative estimation of Cys in a human blood plasma sample.

scholarly journals Analysis of α- and β-amanitin in Human Plasma at Subnanogram per Milliliter Levels by Reversed Phase Ultra-High Performance Liquid Chromatography Coupled to Orbitrap Mass Spectrometry

Toxins ◽  
2020 ◽  
Vol 12 (11) ◽  
pp. 671
Author(s):  
Thomas P. Bambauer ◽  
Lea Wagmann ◽  
Armin A. Weber ◽  
Markus R. Meyer
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Blood Plasma ◽  
Human Plasma ◽  
Human Blood ◽  
Reversed Phase ◽  
Urine Samples ◽  
Human Blood Plasma ◽  
Plasma Samples ◽  
Valuable Alternative

Amatoxins are known to be one of the main causes of serious to fatal mushroom intoxication. Thorough treatment, analytical confirmation, or exclusion of amatoxin intake is crucial in the case of any suspected mushroom poisoning. Urine is often the preferred matrix due to its higher concentrations compared to other body fluids. If urine is not available, analysis of human blood plasma is a valuable alternative for assessing the severity of intoxications. The aim of this study was to develop and validate a liquid chromatography (LC)-high resolution tandem mass spectrometry (HRMS/MS) method for confirmation and quantitation of α- and β-amanitin in human plasma at subnanogram per milliliter levels. Plasma samples of humans after suspected intake of amatoxin-containing mushrooms should be analyzed and amounts of toxins compared with already published data as well as with matched urine samples. Sample preparation consisted of protein precipitation, aqueous liquid-liquid extraction, and solid-phase extraction. Full chromatographical separation of analytes was achieved using reversed-phase chromatography. Orbitrap-based MS allowed for sufficiently sensitive identification and quantification. Validation was successfully carried out, including analytical selectivity, carry-over, matrix effects, accuracy, precision, and dilution integrity. Limits of identification were 20 pg/mL and calibration ranged from 20 pg/mL to 2000 pg/mL. The method was applied to analyze nine human plasma samples that were submitted along with urine samples tested positive for amatoxins. α-Amanitin could be identified in each plasma sample at a range from 37–2890 pg/mL, and β-amanitin was found in seven plasma samples ranging from <20–7520 pg/mL. A LC-HRMS/MS method for the quantitation of amatoxins in human blood plasma at subnanogram per milliliter levels was developed, validated, and used for the analysis of plasma samples. The method provides a valuable alternative to urine analysis, allowing thorough patient treatment but also further study the toxicokinetics of amatoxins.

A novel fluorescence probe for estimation of cysteine/histidine in human blood plasma and recognition of endogenous cysteine in live Hct116 cells

2014 ◽  
Vol 50 (69) ◽  
pp. 9899-9902 ◽  
Author(s):  
Upendar Reddy G. ◽  
Hridesh Agarwalla ◽  
Nandaraj Taye ◽  
Suvankar Ghorai ◽  
Samit Chattopadhyay ◽  
...  
Keyword(s):  
Cell Membrane ◽  
Blood Plasma ◽  
Human Blood ◽  
Plasma Sample ◽  
Fluorescence Probe ◽  
Quantitative Estimation ◽  
Human Blood Plasma ◽  
Blood Plasma Sample ◽  
Hct116 Cells

A new cell membrane permeable reagent for detection of endogenous Cys as well as quantitative estimation of Cys and His in a human blood plasma sample.

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