scholarly journals 19F NMR indicator displacement assay using a synthetic receptor with appended paramagnetic relaxation agent

2014 ◽  
Vol 50 (72) ◽  
pp. 10499-10501 ◽  
Author(s):  
Adam J. Plaunt ◽  
Kasey J. Clear ◽  
Bradley D. Smith
Keyword(s):  
Signal Intensity ◽  
Paramagnetic Relaxation ◽  
19F Nmr ◽  
Synthetic Receptor ◽  
Displacement Assay

A 19F NMR indicator displacement assay detects phosphorylated analytes with amplified signal intensity.

scholarly journals Estimating the cooperativity of PROTAC-induced ternary complexes using 19F NMR displacement assay

2021 ◽  
Author(s):  
Guilherme Castro ◽  
Alessio Ciulli
Keyword(s):  
Ternary Complexes ◽  
19F Nmr ◽  
Fluorine Nmr ◽  
Competition Binding ◽  
Displacement Assay

Cooperativity is an important parameter to understand the ternary complexes formed by protein degraders. We developed fluorine NMR competition binding experiments to determine cooperativity of PROTACs. We show applicability to...

scholarly journals Colorimetric Sensing of Amoxicillin Facilitated by Molecularly Imprinted Polymers

Polymers ◽  
2021 ◽  
Vol 13 (13) ◽  
pp. 2221
Author(s):  
Joseph W Lowdon ◽  
Hanne Diliën ◽  
Bart van Grinsven ◽  
Kasper Eersels ◽  
Thomas J. Cleij
Keyword(s):  
Binding Capacity ◽  
Colorimetric Detection ◽  
Bulk Polymerization ◽  
Colorimetric Sensing ◽  
Initial Development ◽  
Molecularly Imprinted ◽  
Synthetic Receptor ◽  
Displacement Assay ◽  
Size And Morphology ◽  
Task Sets

The scope of the presented research orientates itself towards the development of a Molecularly Imprinted Polymer (MIP)-based dye displacement assay for the colorimetric detection of the antibiotic amoxicillin in aqueous medium. With this in mind, the initial development of an MIP capable of such a task sets focus on monolithic bulk polymerization to assess monomer/crosslinker combinations that have potential towards the binding of amoxicillin. The best performing composition (based on specificity and binding capacity) is utilized in the synthesis of MIP particles by emulsion polymerization, yielding particles that prove to be more homogenous in size and morphology compared to that of the crushed monolithic MIP, which is an essential trait when it comes to the accuracy of the resulting assay. The specificity and selectivity of the emulsion MIP proceeds to be highlighted, demonstrating a higher affinity towards amoxicillin compared to other compounds of the aminopenicillin class (ampicillin and cloxacillin). Conversion of the polymeric receptor is then undertaken, identifying a suitable dye for the displacement assay by means of binding experiments with malachite green, crystal violet, and mordant orange. Once identified, the optimal dye is then loaded onto the synthetic receptor, and the displaceability of the dye deduced by means of a dose response experiment. Alongside the sensitivity, the selectivity of the assay is scrutinized against cloxacillin and ampicillin. Yielding a dye displacement assay that can be used (semi-)quantitatively in a rapid manner.

scholarly journals Site selective reading of epigenetic markers by a dual-mode synthetic receptor array

2017 ◽  
Vol 8 (5) ◽  
pp. 3960-3970 ◽  
Author(s):  
Yang Liu ◽  
Lizeth Perez ◽  
Magi Mettry ◽  
Adam D. Gill ◽  
Samantha R. Byers ◽  
...  
Keyword(s):  
Assay System ◽  
Dual Mode ◽  
Epigenetic Markers ◽  
Synthetic Receptor ◽  
Receptor Array ◽  
Displacement Assay ◽  
Site Selective

Arrayed, self-folding deep cavitands form a fluorescence displacement assay system for the site-selective sensing of post-translationally modified (PTM) histone peptides.

Effects of vasodilators on the signal intensity of perfluorocarbon monitored by in vivo 19F-NMR spectroscopy

1997 ◽  
Vol 15 (3) ◽  
pp. 341-345 ◽  
Author(s):  
Takayuki Sogabe ◽  
Takashi Imaizumi ◽  
Toyoki Mori ◽  
Michiaki Tominaga ◽  
Keiko Koga ◽  
...  
Keyword(s):  
Nmr Spectroscopy ◽  
Signal Intensity ◽  
19F Nmr ◽  
19F Nmr Spectroscopy

scholarly journals A Molecularly Imprinted Polymer-based Dye Displacement Assay for the Rapid Visual Detection of Amphetamine in Urine

Molecules ◽  
2020 ◽  
Vol 25 (22) ◽  
pp. 5222
Author(s):  
Joseph W. Lowdon ◽  
Kasper Eersels ◽  
Rocio Arreguin-Campos ◽  
Manlio Caldara ◽  
Benjamin Heidt ◽  
...  
Keyword(s):  
Molecularly Imprinted Polymers ◽  
Limit Of Detection ◽  
Low Cost ◽  
Molecularly Imprinted ◽  
Selective Method ◽  
Design And Optimization ◽  
Synthetic Receptor ◽  
Displacement Assay ◽  
Selection For ◽  
Visual Confirmation

The rapid sensing of drug compounds has traditionally relied on antibodies, enzymes and electrochemical reactions. These technologies can frequently produce false positives/negatives and require specific conditions to operate. Akin to antibodies, molecularly imprinted polymers (MIPs) are a more robust synthetic alternative with the ability to bind a target molecule with an affinity comparable to that of its natural counterparts. With this in mind, the research presented in this article introduces a facile MIP-based dye displacement assay for the detection of (±) amphetamine in urine. The selective nature of MIPs coupled with a displaceable dye enables the resulting low-cost assay to rapidly produce a clear visual confirmation of a target’s presence, offering huge commercial potential. The following manuscript characterizes the proposed assay, drawing attention to various facets of the sensor design and optimization. To this end, synthesis of a MIP tailored towards amphetamine is described, scrutinizing the composition and selectivity (ibuprofen, naproxen, 2-methoxphenidine, quetiapine) of the reported synthetic receptor. Dye selection for the development of the displacement assay follows, proceeded by optimization of the displacement process by investigating the time taken and the amount of MIP powder required for optimum displacement. An optimized dose–response curve is then presented, introducing (±) amphetamine hydrochloride (0.01–1 mg mL−1) to the engineered sensor and determining the limit of detection (LoD). The research culminates in the assay being used for the analysis of spiked urine samples (amphetamine, ibuprofen, naproxen, 2-methoxphenidine, quetiapine, bupropion, pheniramine, bromopheniramine) and evaluating its potential as a low-cost, rapid and selective method of analysis.

scholarly journals Paramagnetic relaxation enhancement for protein-observed 19F NMR as an enabling approach for efficient fragment screening

RSC Advances ◽  
2016 ◽  
Vol 6 (98) ◽  
pp. 95715-95721 ◽  
Author(s):  
Laura M. L. Hawk ◽  
Clifford T. Gee ◽  
Andrew K. Urick ◽  
Haitao Hu ◽  
William C. K. Pomerantz
Keyword(s):  
Paramagnetic Relaxation ◽  
Paramagnetic Relaxation Enhancement ◽  
19F Nmr ◽  
Fragment Screening ◽  
P Protein ◽  
Ligand Discovery

Protein-observed 19F (PrOF) NMR is an emerging tool for ligand discovery.

scholarly journals Correction: Estimating the cooperativity of PROTAC-induced ternary complexes using 19F NMR displacement assay

2022 ◽  
Author(s):  
Guilherme Vieira de Castro ◽  
Alessio Ciulli
Keyword(s):  
Ternary Complexes ◽  
19F Nmr ◽  
Displacement Assay

Correction for ‘Estimating the cooperativity of PROTAC-induced ternary complexes using 19F NMR displacement assay’ by Guilherme Vieira de Castro et al., RSC Med. Chem., 2021, 12, 1765–1770, DOI: 10.1039/D1MD00215E.

Influence of preoperative statins and aspirin administration on biological and magnetic resonance imaging properties in patients with abdominal aortic aneurysm

VASA ◽  
2020 ◽  
pp. 1-9
Author(s):  
Milos Sladojevic ◽  
Petar Zlatanovic ◽  
Zeljka Stanojevic ◽  
Igor Koncar ◽  
Sasenka Vidicevic ◽  
...  
Keyword(s):  
Magnetic Resonance Imaging ◽  
Abdominal Aortic Aneurysm ◽  
Magnetic Resonance ◽  
Aortic Aneurysm ◽  
Signal Intensity ◽  
Psoas Muscle ◽  
Resonance Imaging ◽  
Maximal Diameter ◽  
Aneurysm Wall ◽  
Abdominal Aortic

Summary: Background: Main objective of this study was to evaluate the influence of statins and/or acetylsalicylic acid on biochemical characteristics of abdominal aortic aneurysm (AAA) wall and intraluminal thrombus (ILT). Patients and methods: Fifty patients with asymptomatic infrarenal AAA were analyzed using magnetic resonance imaging on T1w sequence. Relative ILT signal intensity (SI) was determined as a ratio between ILT and psoas muscle SI. Samples containing the full ILT thickness and aneurysm wall were harvested from the anterior surface at the level of the maximal diameter. The concentration of enzymes such as matrix metalloproteinase (MMP) 9, MMP2 and neutrophil elastase (NE/ELA) were analyzed in ILT and AAA wall; while collagen type III, elastin and proteoglycan 4 were analyzed in harvested AAA wall. Oxidative stress in the AAA wall was assessed by catalase and malondialdehyde activity in tissue samples. Results: Relative ILT signal intensity (1.09 ± 0.41 vs 0.89 ± 0.21, p = 0.013) were higher in non-statin than in statin group. Patients who were taking aspirin had lower relative ILT area (0.89 ± 0.19 vs 1.13. ± 0.44, p = 0.016), and lower relative ILT signal intensity (0.85 [0.73–1.07] vs 1.01 [0.84–1.19], p = 0.021) compared to non-aspirin group. There were higher concentrations of elastin in AAA wall among patients taking both of aspirin and statins (1.21 [0.77–3.02] vs 0.78 (0.49–1.05) ng/ml, p = 0.044) than in patients who did not take both of these drugs. Conclusions: Relative ILT SI was lower in patients taking statin and aspirin. Combination of antiplatelet therapy and statins was associated with higher elastin concentrations in AAA wall.

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