Determination of newly synthesized lipoic acid–fasudil dimer in rat plasma by LC-MS/MS and its application to pharmacokinetics study

2014 ◽  
Vol 6 (21) ◽  
pp. 8675-8681 ◽  
Author(s):  
Waner Hou ◽  
Guanghui Liu ◽  
Xiuman Sun ◽  
Zhiyong Xie ◽  
Rongbiao Pi ◽  
...  
Keyword(s):  
Electrospray Ionization ◽  
Lipoic Acid ◽  
Internal Standard ◽  
Rat Plasma ◽  
Pharmacokinetics Study

The aim of the present work was to develop and validate an LC-MS/MS method with electrospray ionization (ESI) for the quantification of lipoic acid–fasudil dimer (L–F 001), a novel multi-functional neuroprotective drug in rat plasma, using tetrahydropalmatine as an internal standard.

Determination of lipoic acid in rat plasma by LC-MS/MS with electrospray ionization: Assay development, validation and application to a pharamcokinetic study

2004 ◽  
Vol 18 (9) ◽  
pp. 681-686 ◽  
Author(s):  
Ravi Kumar Trivedi ◽  
Raja Reddy Kallem ◽  
Rao N. V. S. Mamidi ◽  
Ramesh Mullangi ◽  
Nuggehally R. Srinivas
Keyword(s):  
Electrospray Ionization ◽  
Lipoic Acid ◽  
Rat Plasma ◽  
Assay Development

Development and Validation of an LC-MS/MS Method for Simultaneous Determination of Canagliflozin and Metformin HCl in Rat Plasma and its Application

2020 ◽  
Vol 16 (6) ◽  
pp. 752-762
Author(s):  
Vivek Nalawade ◽  
Vaibhav A. Dixit ◽  
Amisha Vora ◽  
Himashu Zade
Keyword(s):  
Internal Standard ◽  
Rat Plasma ◽  
Precipitation Method ◽  
Herbal Extracts ◽  
One Step ◽  
Precision And Accuracy ◽  
Development And Validation ◽  
The Impact ◽  
Metformin Hcl

Background: Food and herbal extracts rich in Quercetin (QRT) are often self-medicated by diabetics and can potentially alter the pharmacokinetics (PK) of Metformin HCl (MET) and Canagliflozin (CNG) leading to food or herb-drug interactions and reduced therapeutic efficacy. However, the impact of these flavonoids on the pharmacokinetic behaviour of MET and CNG is mostly unknown. Methods: A simple one-step protein precipitation method was developed for the determination of MET and CNG from rat plasma. The mobile phase chosen was MeOH 65% and 35% water containing 0.1% formic acid at a flow rate of 1mL/min. Results: The retention time of MET, internal standard (Valsartan) and CNG was 1.83, 6.2 and 8.2 min, respectively. The method was found to be linear in the range of 200 - 8000 ng/mL for CNG and 100 = 4000 ng/ml for MET. Precision and accuracy of the method were below 20% at LLOQ and below 15% for LQC, MQC, and HQC. Conclusion: The method was successfully applied for the determination of PK of MET and CNG by using 100 μL of rat plasma. QRT co-administration affects the PK parameters of MET and CNG. This alteration in PK parameters might be of significant use for clinicians and patients.

Determination of Total Choline by Liquid Chromatography– Electrospray Ionization–Tandem Mass Spectrometry in Infant Formulas

2012 ◽  
Vol 95 (1) ◽  
pp. 157-162 ◽  
Author(s):  
Shishan Fu ◽  
Baohua Tao ◽  
Shiyun Lai ◽  
Jingshun Zhang ◽  
Ren Yiping
Keyword(s):  
Electrospray Ionization ◽  
Neural Development ◽  
Internal Standard ◽  
Water Soluble ◽  
Infant Formulas ◽  
Research Areas ◽  
Hydrolyzed Protein ◽  
Evaluation Matrix ◽  
Single Laboratory

Abstract Choline is a water-soluble nutrient important for infants' brain and neural development. In infant formulas, choline is one of the important fortified nutrients. A single-laboratory validation study conducted an LC-electrospray ionization-MS/MS to determine total choline in infant formulas. Sample preparation was adopted from AOAC Official MethodSM 999.14, and instrumental running conditions were optimized. The LOQ was 0.2 μg/100 g, which is significant for measuring total choline in infant formulas. Average recoveries for milk-, rice-, soybean-, and hydrolyzed protein-based samples ranged from 86.45 ± 6.04% to 108.98 ± 3.68%, with RSD less than 7%. The repeatability RSD (RSDr) range was 0.24–3.59% in within-day evaluation and 1.16–3.24% in day-to-day evaluation. Matrix effect was also investigated, and can be effectively eliminated by using an internal standard. Therefore, this method has high credibility, and could be used as a routine method of quality control, or for clinical studies and other research areas.

Simultaneous determination of chito-oligosaccharide in rat plasma by LC-MS/MS method: Application to a pharmacokinetics study

2021 ◽  
Author(s):  
Pengpeng Zhang ◽  
Liu Shuai ◽  
Shuang Yang ◽  
Yuanhong Wang ◽  
Ting-Fu Jiang ◽  
...  
Keyword(s):  
Simultaneous Determination ◽  
Rat Plasma ◽  
Plasma Samples ◽  
Pharmacokinetics Study ◽  
Sensitive Method

A simple and sensitive method for the simultaneous determination of chito-oligosaccharide (COS) with degrees of polymerization（DPs）from 2 to 7 was developed and used for COS quantification in rat plasma. Samples...

Simultaneous determination of Rivaroxaban and TAK-438 in rat plasma by LC–MS/MS: application to pharmacokinetic interaction study

Bioanalysis ◽  
2020 ◽  
Vol 12 (1) ◽  
pp. 11-22 ◽  
Author(s):  
Libin Wang ◽  
Shouchang Gai ◽  
Xiaorui Zhang ◽  
Xiaohui Xu ◽  
Nan Gou ◽  
...  
Keyword(s):  
Drug Interactions ◽  
Internal Standard ◽  
Pharmacokinetic Interaction ◽  
Rat Plasma ◽  
Pharmacokinetic Parameters ◽  
Interaction Study ◽  
Significant Difference ◽  
Us Fda ◽  
First Time

Aim: A sensitive and reliable LC–MS/MS method has been established and validated to the quantitation of rivaroxaban (RIV) and TAK-438 in rat plasma using carbamazepine as internal standard. Results: The procedure of method validation was conducted according to the guidelines of EMA and US FDA. At the same time, the method was applied to pharmacokinetic interactions study between RIV and TAK-438 for the first time. When RIV and TAK-438 co-administration to rats, main pharmacokinetic parameters of TAK-438 like AUC(0-t), AUC(0-∞) and Cmax had statistically significant increase. The main pharmacokinetic parameters of RIV have no statistically significant difference (p > 0.05) when co-administered except for t1/2 (p < 0.01). Conclusion: The results indicated that drug–drug interactions occurred between RIV and TAK-438 when co-administered to rats.

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