Simultaneous determination of chito-oligosaccharide in rat plasma by LC-MS/MS method: Application to a pharmacokinetics study

2021 ◽  
Author(s):  
Pengpeng Zhang ◽  
Liu Shuai ◽  
Shuang Yang ◽  
Yuanhong Wang ◽  
Ting-Fu Jiang ◽  
...  
Keyword(s):  
Simultaneous Determination ◽  
Rat Plasma ◽  
Plasma Samples ◽  
Pharmacokinetics Study ◽  
Sensitive Method

A simple and sensitive method for the simultaneous determination of chito-oligosaccharide (COS) with degrees of polymerization（DPs）from 2 to 7 was developed and used for COS quantification in rat plasma. Samples...

A HPLC-DAD-MS/MS Method for Simultaneous Determination of Six Active Ingredients of Salviae Miltiorrhizae and Ligustrazine Hydrochloride Injection in Rat Plasma and its Application to Pharmacokinetic Studies

2020 ◽  
Vol 21 ◽  
Author(s):  
Jianyang Pan ◽  
Luquan Zhang ◽  
Difeifei Xiong ◽  
Bailing Li ◽  
Haibin Qu
Keyword(s):  
Simultaneous Determination ◽  
Pharmacokinetic Study ◽  
Rat Plasma ◽  
Array Detector ◽  
Pharmacokinetic Studies ◽  
Plasma Samples ◽  
Active Ingredients ◽  
Salviae Miltiorrhizae ◽  
Salvianolic Acids

Aims: Pharmacokinetic Study of Salviae Miltiorrhizaeand Ligustrazine Hydrochloride injection. For the evaluation of mechanism of action, safety and clinical rational use of Salviae Miltiorrhizae and Ligustrazine Hydrochloride injection. Background: Salviae Miltiorrhizae and Ligustrazine Hydrochloride injection is a compound preparation consisted of Salvia Miltiorrhiza extract and ligustrazine hydrochloride for the treatment of cardiovascular and cerebrovascular diseases in China. Methods: Plasma samples were precipitated with methanol, which was spiked with ascorbic acid and the supernatant was separated on a Waters Cortecs C18 column, by using a gradient mobile phase system of acetonitrile-water containing 0.05% formic acid (v/v). For internal standards, puerarin was selected for the five salvianolic acids, while isofraxidin was used for ligustrazine hydrochloride. Besides, electrospray ionization in negative mode and multiple-reaction monitoring were used to identify and quantify the five salvianolic acids, whereas ligustrazine hydrochloride was quantified at 310 nm using the diode array detector. Results: Noticeably, all calibration curves showed good linearity (R2>0.99) over the concentration range, with a lower limit of quantification between 0.00411 and 0.0369μg/mL for salvianolic acids, and 1.74 μg/mL for ligustrazine hydrochloride. Next, the precision of the developed method was evaluated by intra-and inter-day assays, and the percentage of relative standard deviation was within 10%. Although the extraction efficiency of some salvianolic acids were not very satisfactory, the sensitivity of the analytical method met the analysis requirements of rat plasma samples. Moreover, the validated method was successfully applied to a pharmacokinetic study of Salviae Miltiorrhizae and Ligustrazine Hydrochloride injection in the rat model. Conclusion: Linear pharmacokinetic characteristics were observed for the six active ingredients after intravenous infusion administration in rats, within the dose range examined here. In summary, our study proposed a HPLC-DAD-MS/MS method in the simultaneous determination of multiple ingredients, and demonstrated its applicability in pharmacokinetic studies.

Simultaneous Determination of Evodiamine and Rutaecarpine in Rat Plasma by UPLC-MS-MS and Its Application in a Pharmacokinetics Study

2010 ◽  
Vol 72 (1-2) ◽  
pp. 187-191 ◽  
Author(s):  
Juanjuan Zhao ◽  
Xiaohui Chen ◽  
Chao Wang ◽  
Yuanjian Wang ◽  
Chao Ma ◽  
...  
Keyword(s):  
Simultaneous Determination ◽  
Rat Plasma ◽  
Pharmacokinetics Study

scholarly journals Simultaneous Determination of 11 Compounds in Gualou Guizhi Granule and Pharmacokinetics Study by UPLC-MS/MS

2017 ◽  
Vol 2017 ◽  
pp. 1-13 ◽  
Author(s):  
Chengtao Sun ◽  
Wen Xu ◽  
Yuqin Zhang ◽  
Lishuang Yu ◽  
Miao Ye ◽  
...  
Keyword(s):  
Simultaneous Determination ◽  
Gradient Elution ◽  
Rat Plasma ◽  
Negative Ion ◽  
Detection Range ◽  
Pharmacokinetics Study ◽  
The Matrix ◽  
Liquid Chromatography Tandem Mass ◽  
Negative Ion Mode

A rapid and sensitive ultrafast performance liquid chromatography-tandem mass spectrometry method (UPLC-MS/MS) was developed for the simultaneous determination of 11 compounds in Gualou Guizhi Granule (GLGZG), including liquiritin, isoliquiritin, liquirtin apioside, isoliquiritin apioside, liquiritigenin, isoliquiritigenin, glycyrrhizic acid, glycyrrhetinic acid, paeoniflorin, albiflorin, and paeoniflorin sulfonate in rat plasma. UPLC-MS/MS assay with negative ion mode was performed on a Waters CORTECS C18 (2.1 × 100 mm, 1.6 μm) with the mobile phase consisting of 0.1% aqueous formic acid (A) and acetonitrile (B) in gradient elution at a flow rate of 0.25 mL·min−1. The method was linear for all analytes within the detection range (r≥0.9597). The inter- and intraday precision (RSD) were 2.21–6.41% and 1.67–6.18%; the inter- and intraday accuracy (recover) were 92.48–114.03% and 90.23–112.04%. And the recovery rate ranged from 81.30% to 108.22%. The matrix effect values obtained for analytes ranged from 88.91% to 113.32%. This validated method was successfully applied to a pharmacokinetics study in rats after oral administration of GLGZG.

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