Amplified fluorescence detection of T4 polynucleotide kinase activity and inhibition via a coupled λ exonuclease reaction and exonuclease III-aided trigger DNA recycling

2014 ◽  
Vol 6 (15) ◽  
pp. 6009 ◽  
Author(s):  
Zi-Mao Zhu ◽  
Ru-Qin Yu ◽  
Xia Chu
Keyword(s):  
Fluorescence Detection ◽  
Kinase Activity ◽  
Exonuclease Iii ◽  
Polynucleotide Kinase ◽  
T4 Polynucleotide Kinase

An amplified fluorescence detection of T4 polynucleotide kinase activity based on coupled exonuclease III reaction and a graphene oxide platform

The Analyst ◽  
2015 ◽  
Vol 140 (6) ◽  
pp. 1827-1831 ◽  
Author(s):  
Ni-Na Sun ◽  
Rong-Mei Kong ◽  
Fengli Qu ◽  
Xiaobing Zhang ◽  
Shufang Zhang ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Fluorescence Detection ◽  
Kinase Activity ◽  
Sensitive Detection ◽  
Exonuclease Iii ◽  
Sensing System ◽  
Polynucleotide Kinase ◽  
T4 Polynucleotide Kinase

A novel amplified fluorescence graphene oxide (GO) sensing system for sensitive detection of T4 polynucleotide kinase (PNK) activity and inhibition was developed based on the exonuclease III (ExoIII) reaction.

Label-free fluorescence assay coupled exonuclease reaction and SYBR Green I for the detection of T4 polynucleotide kinase activity

2020 ◽  
Vol 12 (6) ◽  
pp. 807-812
Author(s):  
Xu Wu ◽  
Shuyi He ◽  
Julia Xiaojun Zhao
Keyword(s):  
Kinase Activity ◽  
Sybr Green ◽  
Fluorescence Assay ◽  
Sybr Green I ◽  
Label Free ◽  
Cleavage Reaction ◽  
Polynucleotide Kinase ◽  
T4 Polynucleotide Kinase

A sensitive label-free fluorescence assay for monitoring T4 polynucleotide kinase (T4 PNK) activity and inhibition was developed based on a coupled λ exonuclease cleavage reaction and SYBR Green I.

Exonuclease III-assisted signal amplification strategy for sensitive fluorescence detection of polynucleotide kinase based on poly(thymine)-templated copper nanoparticles

The Analyst ◽  
2019 ◽  
Vol 144 (22) ◽  
pp. 6689-6697 ◽  
Author(s):  
Han Zhao ◽  
Ying Yan ◽  
Mingjian Chen ◽  
Tingting Hu ◽  
Kefeng Wu ◽  
...  
Keyword(s):  
Fluorescence Detection ◽  
Copper Nanoparticles ◽  
Signal Amplification ◽  
Exonuclease Iii ◽  
Fluorescent Method ◽  
Polynucleotide Kinase ◽  
Amplification Strategy

A facile fluorescent method has been developed for polynucleotide kinase detection based on copper nanoparticles and exonuclease III-assisted signal amplification.

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