Label-free fluorescence assay coupled exonuclease reaction and SYBR Green I for the detection of T4 polynucleotide kinase activity

2020 ◽  
Vol 12 (6) ◽  
pp. 807-812
Author(s):  
Xu Wu ◽  
Shuyi He ◽  
Julia Xiaojun Zhao
Keyword(s):  
Kinase Activity ◽  
Sybr Green ◽  
Fluorescence Assay ◽  
Sybr Green I ◽  
Label Free ◽  
Cleavage Reaction ◽  
Polynucleotide Kinase ◽  
T4 Polynucleotide Kinase

A sensitive label-free fluorescence assay for monitoring T4 polynucleotide kinase (T4 PNK) activity and inhibition was developed based on a coupled λ exonuclease cleavage reaction and SYBR Green I.

Label-free luminescence switch-on detection of T4 polynucleotide kinase activity using a G-quadruplex-selective probe

2014 ◽  
Vol 50 (40) ◽  
pp. 5313-5315 ◽  
Author(s):  
Hong-Zhang He ◽  
Ka-Ho Leung ◽  
Wei Wang ◽  
Daniel Shiu-Hin Chan ◽  
Chung-Hang Leung ◽  
...  
Keyword(s):  
Kinase Activity ◽  
Label Free ◽  
Polynucleotide Kinase ◽  
G Quadruplex ◽  
T4 Polynucleotide Kinase

Label-free fluorescence light-up detection of T4 polynucleotide kinase activity using the split-to-intact G-quadruplex strategy by ligation-triggered and toehold-mediated strand displacement release

The Analyst ◽  
2015 ◽  
Vol 140 (16) ◽  
pp. 5450-5453 ◽  
Author(s):  
Lu Zhou ◽  
Xiaoqiang Shen ◽  
Na Sun ◽  
Kewei Wang ◽  
Yuanyuan Zhang ◽  
...  
Keyword(s):  
Kinase Activity ◽  
Detection Method ◽  
Label Free ◽  
Strand Displacement ◽  
Polynucleotide Kinase ◽  
G Quadruplex ◽  
Fluorescence Light ◽  
T4 Polynucleotide Kinase

A label-free, fluorescence light-up detection method for T4 polynucleotide kinase activity has been developed using the split-to-intact G-quadruplex strategy.

Amplified Detection of T4 Polynucleotide Kinase Activity by the Coupled λ Exonuclease Cleavage Reaction and Catalytic Assembly of Bimolecular Beacons

2013 ◽  
Vol 86 (1) ◽  
pp. 884-890 ◽  
Author(s):  
Ting Hou ◽  
Xiuzhong Wang ◽  
Xiaojuan Liu ◽  
Tingting Lu ◽  
Shufeng Liu ◽  
...  
Keyword(s):  
Kinase Activity ◽  
Cleavage Reaction ◽  
Polynucleotide Kinase ◽  
T4 Polynucleotide Kinase

A label-free cyclic assembly of G-quadruplex nanowires for cascade amplification detection of T4 polynucleotide kinase activity and inhibition

The Analyst ◽  
2015 ◽  
Vol 140 (17) ◽  
pp. 6124-6130 ◽  
Author(s):  
Zhilu Shi ◽  
Xiafei Zhang ◽  
Rui Cheng ◽  
Baoxin Li ◽  
Yan Jin
Keyword(s):  
Kinase Activity ◽  
Hybridization Chain Reaction ◽  
Label Free ◽  
Chain Reaction ◽  
Polynucleotide Kinase ◽  
G Quadruplex ◽  
Amplification Strategy ◽  
Cascade Amplification ◽  
T4 Polynucleotide Kinase

A label-free and enzyme-free amplification strategy has been developed for sensitively and specifically studying PNK activity and inhibitionviathe hybridization chain reaction (HCR).

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