Highly specific fluorescence detection of T4 polynucleotide kinase activity via photo-induced electron transfer

2015 ◽  
Vol 485 ◽  
pp. 18-24 ◽  
Author(s):  
Mangjuan Tao ◽  
Zhilu Shi ◽  
Rui Cheng ◽  
Jing Zhang ◽  
Baoxin Li ◽  
...  
Keyword(s):  
Electron Transfer ◽  
Fluorescence Detection ◽  
Kinase Activity ◽  
Specific Fluorescence ◽  
Polynucleotide Kinase ◽  
Photo Induced Electron Transfer ◽  
T4 Polynucleotide Kinase

An amplified fluorescence detection of T4 polynucleotide kinase activity based on coupled exonuclease III reaction and a graphene oxide platform

The Analyst ◽  
2015 ◽  
Vol 140 (6) ◽  
pp. 1827-1831 ◽  
Author(s):  
Ni-Na Sun ◽  
Rong-Mei Kong ◽  
Fengli Qu ◽  
Xiaobing Zhang ◽  
Shufang Zhang ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Fluorescence Detection ◽  
Kinase Activity ◽  
Sensitive Detection ◽  
Exonuclease Iii ◽  
Sensing System ◽  
Polynucleotide Kinase ◽  
T4 Polynucleotide Kinase

A novel amplified fluorescence graphene oxide (GO) sensing system for sensitive detection of T4 polynucleotide kinase (PNK) activity and inhibition was developed based on the exonuclease III (ExoIII) reaction.

Label-free fluorescence assay coupled exonuclease reaction and SYBR Green I for the detection of T4 polynucleotide kinase activity

2020 ◽  
Vol 12 (6) ◽  
pp. 807-812
Author(s):  
Xu Wu ◽  
Shuyi He ◽  
Julia Xiaojun Zhao
Keyword(s):  
Kinase Activity ◽  
Sybr Green ◽  
Fluorescence Assay ◽  
Sybr Green I ◽  
Label Free ◽  
Cleavage Reaction ◽  
Polynucleotide Kinase ◽  
T4 Polynucleotide Kinase

A sensitive label-free fluorescence assay for monitoring T4 polynucleotide kinase (T4 PNK) activity and inhibition was developed based on a coupled λ exonuclease cleavage reaction and SYBR Green I.

A fluorescence-based assay for T4 polynucleotide kinase/phosphatase activity based on a terminal transferase-aided photoinduced electron transfer strategy

2016 ◽  
Vol 8 (9) ◽  
pp. 1989-1994 ◽  
Author(s):  
Changbei Ma ◽  
Shunxin Jin ◽  
Jun Wang ◽  
Kemin Wang ◽  
Haisheng Liu ◽  
...  
Keyword(s):  
Electron Transfer ◽  
Phosphatase Activity ◽  
Photoinduced Electron Transfer ◽  
Fluorescence Method ◽  
Terminal Transferase ◽  
Polynucleotide Kinase ◽  
T4 Polynucleotide Kinase

A novel fluorescence method for detection of T4 polynucleotide kinase/phosphatase activity based on a terminal transferase-aided photoinduced electron transfer strategy.

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