Dual-stimuli reduction and acidic pH-responsive bionanogels: intracellular delivery nanocarriers with enhanced release

RSC Advances ◽  
2014 ◽  
Vol 4 (1) ◽  
pp. 229-237 ◽  
Author(s):  
Yifen Wen ◽  
Jung Kwon Oh
Keyword(s):  
Intracellular Delivery ◽  
Acidic Ph ◽  
Ph Responsive

Acidic pH-responsive changes of DNA structure and surface dynamics as probed with ultrasensitive Raman spectroscopy

2021 ◽  
Vol 258 ◽  
pp. 119866
Author(s):  
Cristina M. Muntean ◽  
Nicoleta E. Dina ◽  
Ioan Bratu ◽  
Carmen Tripon ◽  
Sorina Niţu (Năstase) ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Dna Structure ◽  
Acidic Ph ◽  
Surface Dynamics ◽  
Ph Responsive

Real time monitoring of peptide delivery in vitro using high payload pH responsive nanogels

2020 ◽  
Vol 11 (2) ◽  
pp. 425-432 ◽  
Author(s):  
Shegufta Farazi ◽  
Fan Chen ◽  
Henry Foster ◽  
Raelene Boquiren ◽  
Shelli R. McAlpine ◽  
...  
Keyword(s):  
Real Time ◽  
Intracellular Delivery ◽  
Peptide Delivery ◽  
High Loading ◽  
Loading Capacity ◽  
Ph Responsive ◽  
Real Time Monitoring ◽  
High Payload

A pH responsive pMAA nanogel that demonstrates high loading capacity and rapid intracellular delivery of hydrophilic peptides.

A versatile pH-responsive platform for intracellular protein delivery using calcium phosphate nanoparticles

2015 ◽  
Vol 3 (47) ◽  
pp. 9115-9121 ◽  
Author(s):  
Bingru Zeng ◽  
Hongdong Shi ◽  
Yangzhong Liu
Keyword(s):  
Calcium Phosphate ◽  
Protein Delivery ◽  
Intracellular Delivery ◽  
Endosomal Escape ◽  
Ph Responsive ◽  
Intracellular Protein ◽  
Calcium Phosphate Nanoparticles ◽  
Intracellular Protein Delivery

A highly biocompatible nanoplatform for the intracellular delivery of different proteins, exhibiting pH-responsive release and efficient endosomal escape.

scholarly journals PAC1, a pH-Regulatory Gene from Fusarium verticillioides

2003 ◽  
Vol 69 (9) ◽  
pp. 5222-5227 ◽  
Author(s):  
Joseph E. Flaherty ◽  
Anna Maria Pirttilä ◽  
Burton H. Bluhm ◽  
Charles P. Woloshuk
Keyword(s):  
Fusarium Verticillioides ◽  
Regulatory Gene ◽  
Synthetic Medium ◽  
Transcriptional Regulators ◽  
Acidic Ph ◽  
Alkaline Ph ◽  
Wild Type ◽  
Ph Responsive ◽  
Alkaline Conditions ◽  
Maize Kernels

ABSTRACT Fumonisins are a group of mycotoxins that contaminate maize and cause leukoencephalomalacia in equine, pulmonary edema in swine, and promote cancer in mice. Fumonisin biosynthesis in Fusarium verticillioides is repressed by nitrogen and alkaline pH. We cloned a PACC-like gene (PAC1) from F. verticillioides. PACC genes encode the major transcriptional regulators of several pH-responsive pathways in other filamentous fungi. In Northern blot analyses, a PAC1 probe hybridized to a 2.2-kb transcript present in F. verticillioides grown at alkaline pH. A mutant of F. verticillioides with a disrupted PAC1 gene had severely impaired growth at alkaline pH. The mutant produced more fumonisin than the wild type when grown on maize kernels and in a synthetic medium buffered at an acidic pH, 4.5. The mutant, but not the wild type, also produced fumonisin B1 when mycelia were resuspended in medium buffered at an alkaline pH, 8.4. Transcription of FUM1, a gene involved in fumonisin biosynthesis, was correlated with fumonisin production. We conclude that PAC1 is required for growth at alkaline pH and that Pac1 may have a role as a repressor of fumonisin biosynthesis under alkaline conditions.

Dual pH-responsive and CD44 receptor targeted multifunctional nanoparticles for anticancer intracellular delivery

2014 ◽  
Vol 16 (11) ◽  
Author(s):  
Daquan Chen ◽  
Jingfang Sun ◽  
Shengnan Lian ◽  
Zongliang Liu ◽  
Kaoxiang Sun ◽  
...  
Keyword(s):  
Intracellular Delivery ◽  
Ph Responsive ◽  
Multifunctional Nanoparticles ◽  
Cd44 Receptor

Acidic pH-Responsive siRNA Conjugate for Reversible Carrier Stability and Accelerated Endosomal Escape with Reduced IFNα-Associated Immune Response

2013 ◽  
Vol 52 (24) ◽  
pp. 6218-6221 ◽  
Author(s):  
Hiroyasu Takemoto ◽  
Kanjiro Miyata ◽  
Shota Hattori ◽  
Takehiko Ishii ◽  
Tomoya Suma ◽  
...  
Keyword(s):  
Immune Response ◽  
Endosomal Escape ◽  
Acidic Ph ◽  
Ph Responsive

Tumoral acidic pH-responsive MPEG-poly(β-amino ester) polymeric micelles for cancer targeting therapy

2010 ◽  
Vol 144 (2) ◽  
pp. 259-266 ◽  
Author(s):  
Kyung Hyun Min ◽  
Jong-Ho Kim ◽  
Sang Mun Bae ◽  
Hyeri Shin ◽  
Min Sang Kim ◽  
...  
Keyword(s):  
Polymeric Micelles ◽  
Cancer Targeting ◽  
Acidic Ph ◽  
Amino Ester ◽  
Ph Responsive ◽  
Targeting Therapy

The pH-Responsive Property of Antimicrobial Peptide GH12 Enhances Its Anticaries Effects at Acidic pH

2020 ◽  
pp. 1-11
Author(s):  
Wentao Jiang ◽  
Junyuan Luo ◽  
Yufei Wang ◽  
Xiangshu Chen ◽  
Xuelian Jiang ◽  
...  
Keyword(s):  
Antimicrobial Peptide ◽  
Lipid Membranes ◽  
Lactic Acid Production ◽  
Tryptophan Fluorescence ◽  
Term Treatment ◽  
Tooth Surface ◽  
Acidic Ph ◽  
Ph Responsive ◽  
Short Term Treatment ◽  
Minimal Inhibitory Concentrations

Dental caries is closely related to the acidification of the biofilms on the tooth surface, in which cariogenic bacteria bring about a dramatic pH decrease and disrupt remineralisation equilibrium upon the fermentation of dietary sugars. Thus, approaches targeting the acidified niches with enhanced anticaries activities at acidic pH are highly desirable. In our previous study, a cationic amphipathic α-helical antimicrobial peptide GH12 (Gly-Leu-Leu-Trp-His-Leu-Leu-His-His-Leu-Leu-His-NH<sub>2</sub>) was designed with good stability, low cytotoxicity, and excellent antibacterial effects. Considering its potent antibacterial activity against the acidogenic bacteria and its histidine-rich sequence, it was speculated that GH12 might show enhanced antimicrobial effects at an acidic pH. In this study, the pH-responsive property of GH12 was determined to evaluate its potential as a smart acid-activated anticaries agent. GH12 possessed much lower minimal inhibitory concentrations and minimal bactericidal concentrations against various kinds of bacteria at pH 5.5 than at pH 7.2. Employing <i>Streptococcus mutans</i>, the principal caries pathogen, as the model system, it was found that GH12 showed much stronger bactericidal effects on both planktonic <i>S. mutans</i> and <i>S. mutans</i> embedded in the biofilm at pH 5.5. In addition, short-term treatment with GH12 showed much more effective inhibitory effects on water-insoluble exopolysaccharides synthesis and lactic acid production of the preformed <i>S. mutans</i> biofilm at pH 5.5. As for the mechanism exploration, it was found that the net positive charge of GH12 increased and the tryptophan fluorescence intensity heightened with the peak shifting towards the short wavelength at pH 5.5, which demonstrated that GH12 could be more easily attracted to the anionic microbial cell membranes and that GH12 showed stronger interactions with the lipid membranes. In conclusion, acidic pH enhanced the antibacterial and antibiofilm activities of GH12, and GH12 is a potential smart anticaries agent targeting the cariogenic acidic microenvironment.

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