A versatile pH-responsive platform for intracellular protein delivery using calcium phosphate nanoparticles

2015 ◽  
Vol 3 (47) ◽  
pp. 9115-9121 ◽  
Author(s):  
Bingru Zeng ◽  
Hongdong Shi ◽  
Yangzhong Liu
Keyword(s):  
Calcium Phosphate ◽  
Protein Delivery ◽  
Intracellular Delivery ◽  
Endosomal Escape ◽  
Ph Responsive ◽  
Intracellular Protein ◽  
Calcium Phosphate Nanoparticles ◽  
Intracellular Protein Delivery

A highly biocompatible nanoplatform for the intracellular delivery of different proteins, exhibiting pH-responsive release and efficient endosomal escape.

scholarly journals Cytosolic protein delivery using pH-responsive, charge-reversible lipid nanoparticles

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Yusuke Hirai ◽  
Hisaaki Hirose ◽  
Miki Imanishi ◽  
Tomohiro Asai ◽  
Shiroh Futaki
Keyword(s):  
Protein Delivery ◽  
Electrostatic Interactions ◽  
Fluorescent Protein ◽  
Lipid Nanoparticles ◽  
Endosomal Escape ◽  
Ph Responsive ◽  
Cargo Proteins ◽  
Green Fluorescent ◽  
Intracellular Protein Delivery ◽  
Endosomal Release

AbstractAlthough proteins have attractive features as biopharmaceuticals, the difficulty in delivering them into the cell interior limits their applicability. Lipid nanoparticles (LNPs) are a promising class of delivery vehicles. When designing a protein delivery system based on LNPs, the major challenges include: (i) formulation of LNPs with defined particle sizes and dispersity, (ii) efficient encapsulation of cargo proteins into LNPs, and (iii) effective cellular uptake and endosomal release into the cytosol. Dioleoylglycerophosphate-diethylenediamine (DOP-DEDA) is a pH-responsive, charge-reversible lipid. The aim of this study was to evaluate the applicability of DOP-DEDA-based LNPs for intracellular protein delivery. Considering the importance of electrostatic interactions in protein encapsulation into LNPs, a negatively charged green fluorescent protein (GFP) analog was successfully encapsulated into DOP-DEDA-based LNPs to yield diameters and polydispersity index of < 200 nm and < 0.2, respectively. Moreover, ~ 80% of the cargo proteins was encapsulated into the LNPs. Cytosolic distribution of fluorescent signals of the protein was observed for up to ~ 90% cells treated with the LNPs, indicating the facilitated endocytic uptake and endosomal escape of the cargo attained using the LNP system.

An intracellular protein delivery platform based on glutathione-responsive protein nanocapsules

2016 ◽  
Vol 52 (93) ◽  
pp. 13608-13611 ◽  
Author(s):  
Jie Li ◽  
Linlin Zhang ◽  
Yang Liu ◽  
Jing Wen ◽  
Di Wu ◽  
...  
Keyword(s):  
Protein Delivery ◽  
Intracellular Delivery ◽  
Intracellular Protein ◽  
Disulfide Crosslinking ◽  
Efficient Strategy ◽  
Protein Polymer ◽  
Delivery Platform ◽  
The Stability ◽  
Intracellular Protein Delivery ◽  
Polymer Assembly

We reported an efficient strategy for the intracellular delivery of proteins based on assembling proteins with a self-crosslinkable polymer. The disulfide-crosslinking structure enhances the stability of the protein–polymer assembly, and also allows effective dissociation of the assembly in response to glutathione.

Combinatorial synthesis of redox-responsive cationic polypeptoids for intracellular protein delivery application

2020 ◽  
Vol 63 (11) ◽  
pp. 1619-1625
Author(s):  
Zhicheng Le ◽  
Tong Xiao ◽  
Zhijia Liu ◽  
Xingliang Liu ◽  
Hong Liu ◽  
...  
Keyword(s):  
Protein Delivery ◽  
Combinatorial Synthesis ◽  
Intracellular Protein ◽  
Redox Responsive ◽  
Intracellular Protein Delivery

Photosensitizer-Bacteria Biohybrids Promote Photodynamic Cancer Cell Ablation and Intracellular Protein Delivery

2019 ◽  
Vol 31 (18) ◽  
pp. 7212-7220 ◽  
Author(s):  
Min Wu ◽  
Wenbo Wu ◽  
Yukun Duan ◽  
Xueqi Li ◽  
Guobin Qi ◽  
...  
Keyword(s):  
Cancer Cell ◽  
Protein Delivery ◽  
Intracellular Protein ◽  
Cell Ablation ◽  
Intracellular Protein Delivery
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