Enhanced Intracellular Delivery of Quantum Dot and Adenovirus Nanoparticles Triggered by Acidic pH via Surface Charge Reversal

2008 ◽  
Vol 19 (4) ◽  
pp. 797-801 ◽  
Author(s):  
Hyejung Mok ◽  
Ji Won Park ◽  
Tae Gwan Park
Keyword(s):  
Quantum Dot ◽  
Surface Charge ◽  
Intracellular Delivery ◽  
Acidic Ph ◽  
Charge Reversal

scholarly journals Self-Assembled Quantum Dot−Peptide Bioconjugates for Selective Intracellular Delivery

2006 ◽  
Vol 17 (4) ◽  
pp. 920-927 ◽  
Author(s):  
James B. Delehanty ◽  
Igor L. Medintz ◽  
Thomas Pons ◽  
Florence M. Brunel ◽  
Philip E. Dawson ◽  
...  
Keyword(s):  
Quantum Dot ◽  
Intracellular Delivery ◽  
Self Assembled

Assembly of a CdS quantum dot–TiO2 nanobelt heterostructure for photocatalytic application: towards an efficient visible light photocatalyst via facile surface charge tuning

2015 ◽  
Vol 39 (1) ◽  
pp. 279-286 ◽  
Author(s):  
Junyu Zhang ◽  
Fang-Xing Xiao ◽  
Guangcan Xiao ◽  
Bin Liu
Keyword(s):  
Quantum Dot ◽  
Visible Light ◽  
Surface Charge ◽  
Self Assembly ◽  
Photocatalytic Performance ◽  
Visible Light Photocatalyst ◽  
Photocatalytic Application ◽  
Cds Quantum Dot

CdS QD sensitized TiO2 nanobelt nanostructures were fabricated by a facile self-assembly approach and the photocatalytic performance of the well-defined hybrid heterostructure was systematically probed.

Intracellular delivery of and sensing with quantum dot bioconjugates

2009 ◽  
Author(s):  
James B. Delehanty ◽  
Christopher E. Bradburne ◽  
Igor L. Medintz ◽  
Dorothy Farrell ◽  
Thomas Pons ◽  
...  
Keyword(s):  
Quantum Dot ◽  
Intracellular Delivery

scholarly journals The optimal pH of AID is skewed from that of its catalytic pocket by DNA-binding residues and surface charge

2021 ◽  
Author(s):  
Atefeh Ghorbani ◽  
Justin John King ◽  
Mani Larijani
Keyword(s):  
Catalytic Activity ◽  
Surface Charge ◽  
Cytidine Deaminase ◽  
Inverse Correlation ◽  
Acidic Ph ◽  
Structural Mechanism ◽  
Positive Surface Charge ◽  
Equivalent Residue ◽  
Binding Residues ◽  
Optimal Ph

Activation-induced cytidine deaminase (AID) is a member of the apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like (APOBEC) family of cytidine deaminases. AID mutates immunoglobulin loci to initiate secondary antibody diversification. The APOBEC3 (A3) sub-branch mutates viral pathogens in the cytosol and acidic endosomal compartments. Accordingly, AID functions optimally near neutral pH, while most A3s are acid-adapted (optimal pH 5.5-6.5). To gain a structural understanding for this pH disparity, we constructed high-resolution maps of AID catalytic activity vs pH. We found AID’s optimal pH was 7.3 but it retained most (>70%) of the activity at pH 8. Probing of ssDNA-binding residues near the catalytic pocket, key for bending ssDNA into the pocket (e.g R25) yielded mutants with altered pH preference, corroborating previous findings that the equivalent residue in APOBEC3G (H216) underlies its acidic pH preference. AID from bony fish exhibited more basic optimal pH (pH 7.5-8.1) and several R25-equivalent mutants altered pH preference. Comparison of pH optima across the AID/APOBEC3 family revealed an inverse correlation between positive surface charge and overall catalysis.  The paralogue with the most robust catalytic activity (APOBEC3A) has the lowest surface charge, most acidic pH preference, while the paralogue with the most lethargic catalytic rate (AID) has the most positive surface charge and highest optimal pH. We suggest one possible mechanism is through surface charge dictating an overall optimal pH that is different from the optimal pH of the catalytic pocket microenvironment. These findings illuminate an additional structural mechanism that regulates AID/APOBEC3 mutagenesis.

scholarly journals Effect of modifying quantum dot surface charge on airway epithelial cell uptakein vitro

2012 ◽  
Vol 7 (6) ◽  
pp. 1143-1151 ◽  
Author(s):  
Eric Chau ◽  
Justin F. Galloway ◽  
Antoinette Nelson ◽  
Patrick N. Breysse ◽  
Denis Wirtz ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Quantum Dot ◽  
Surface Charge ◽  
Airway Epithelial Cell ◽  
Airway Epithelial
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