Universal base analogues and their applications in DNA sequencing technology

RSC Advances ◽  
2013 ◽  
Vol 3 (35) ◽  
pp. 14910 ◽  
Author(s):  
Feng Liang ◽  
Ying-Zhu Liu ◽  
Peiming Zhang
Keyword(s):  
Dna Sequencing ◽  
Sequencing Technology

scholarly journals Current and Future Methodology for Quantitation and Site-specific Mapping the Location of DNA Adducts

2021 ◽  
Author(s):  
Gunnar Boysen ◽  
Intawat Nookaew
Keyword(s):  
Dna Sequencing ◽  
Dna Adducts ◽  
Single Molecule ◽  
State Of The Art ◽  
Dna Adduct ◽  
Sequencing Technology ◽  
Specific Mass ◽  
Comprehensive Picture ◽  
Specific Mapping ◽  
Altered Gene

Abstract: Formation of DNA adducts is a key event for a genotoxic mode of action and its formation is often use as surrogate for mutation and cancer. Interest in DNA adducts are twofold, first, to demonstrate exposure, and second, to link DNA adduct location to subsequent mutations or altered gene regulation. High chemically specific mass spectrometry methods have been established for DNA adduct quantitation and elegant bio-analytic methods utilizing enzymes, various chemistries, and molecular biology methods to visualize the location of DNA adducts. Traditionally, these highly specific methods cannot be combined, and the results are incomparable. Initially developed for single-molecule DNA sequencing, nanopore-type technologies are expected to enable simultaneous quantitation and location of DNA adducts across the genome. We will briefly summarize the current methodologies for state-of-the-art quantitation of DNA adduct levels and mapping of DNA adducts and describe novel single-molecule DNA sequencing technology that is expected to achieve both measures simultaneously. Emerging technologies are expected to soon provide a comprehensive picture of the exposome and identify gene regions susceptible to DNA adduct formation.

Cyanobacterial biodiversity of semiarid public drinking water supply reservoirs assessed via next-generation DNA sequencing technology

2019 ◽  
Vol 57 (6) ◽  
pp. 450-460 ◽  
Author(s):  
Adriana Sturion Lorenzi ◽  
Mathias Ahii Chia ◽  
Fabyano Alvares Cardoso Lopes ◽  
Genivaldo Gueiros Z. Silva ◽  
Robert A. Edwards ◽  
...  
Keyword(s):  
Drinking Water ◽  
Water Supply ◽  
Dna Sequencing ◽  
Drinking Water Supply ◽  
Next Generation ◽  
Sequencing Technology ◽  
Next Generation Dna Sequencing ◽  
Public Drinking Water ◽  
Public Drinking ◽  
Water Supply Reservoirs

scholarly journals Cultivating DNA Sequencing Technology After the Human Genome Project

2020 ◽  
Vol 21 (1) ◽  
pp. 117-138
Author(s):  
Jeffery A. Schloss ◽  
Richard A. Gibbs ◽  
Vinod B. Makhijani ◽  
Andre Marziali
Keyword(s):  
Dna Sequencing ◽  
Human Genome ◽  
Human Genome Project ◽  
Technology Development ◽  
Genome Project ◽  
Alternative Methods ◽  
Biological Research ◽  
Sequencing Technology ◽  
Core Technology ◽  
The Human Genome Project

When the Human Genome Project was completed in 2003, automated Sanger DNA sequencing with fluorescent dye labels was the dominant technology. Several nascent alternative methods based on older ideas that had not been fully developed were the focus of technical researchers and companies. Funding agencies recognized the dynamic nature of technology development and that, beyond the Human Genome Project, there were growing opportunities to deploy DNA sequencing in biological research. Consequently, the National Human Genome Research Institute of the National Institutes of Health created a program—widely known as the Advanced Sequencing Technology Program—that stimulated all stages of development of new DNA sequencing methods, from innovation to advanced manufacturing and production testing, with the goal of reducing the cost of sequencing a human genome first to $100,000 and then to $1,000. The events of this period provide a powerful example of how judicious funding of academic and commercial partners can rapidly advance core technology developments that lead to profound advances across the scientific landscape.

The current approaches to the study of algae: DNA barcoding and DNA taxonomy

2021 ◽  
pp. 124-130
Author(s):  
Anna D. Temraleeva ◽  
Elena S. Krivina ◽  
Yury S. Bukin
Keyword(s):  
Phylogenetic Analysis ◽  
Dna Sequencing ◽  
Dna Barcoding ◽  
Species Identification ◽  
Green Algae ◽  
Molecular Genetic ◽  
Algal Species ◽  
Species Boundaries ◽  
Sequencing Technology ◽  
Dna Taxonomy

The understanding of the impossibility of distinguishing algal species based on morphological features came with the development of DNA sequencing technology, which today is a necessary tool for defining species boundaries and testing traditional species concepts. The paper discusses popular approaches to species identification (DNA barcoding) and the description of new and revision of known species (DNA taxonomy) using molecular genetic methods. The requirements and limitations in their work are given, as well as examples of phylogenetic analysis of green algae from the clade Moewusinia and Parachlorella, including the genus Micractinium.

Advances in DNA sequencing technology

1993 ◽  
Vol 2 (2) ◽  
pp. 82-84
Author(s):  
Norman J. Dovichi
Keyword(s):  
Dna Sequencing ◽  
Sequencing Technology

scholarly journals Solid-State Nanopore-Based DNA Sequencing Technology

2016 ◽  
Vol 2016 ◽  
pp. 1-13 ◽  
Author(s):  
Zewen Liu ◽  
Yifan Wang ◽  
Tao Deng ◽  
Qi Chen
Keyword(s):  
Solid State ◽  
Dna Sequencing ◽  
Surface Properties ◽  
Dna Molecules ◽  
Sequencing Technology ◽  
Gene Detection ◽  
High Quality ◽  
Translocation Velocity ◽  
And Behavior

The solid-state nanopore-based DNA sequencing technology is becoming more and more attractive for its brand new future in gene detection field. The challenges that need to be addressed are diverse: the effective methods to detect base-specific signatures, the control of the nanopore’s size and surface properties, and the modulation of translocation velocity and behavior of the DNA molecules. Among these challenges, the realization of the high-quality nanopores with the help of modern micro/nanofabrication technologies is a crucial one. In this paper, typical technologies applied in the field of solid-state nanopore-based DNA sequencing have been reviewed.

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